본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

느타리버섯균의 핵의 동태에 관한 미세구조적 연구 -균사분화중의 성상체 미세소관에 관한 연구-
Ultrastructural Aspects of Nuclear Behaviors of Pleurotus ostreatus - Behaviors of Astral Microtubules During Hyphal Development -

윤권상    (강원대학교 미생물학과  );
  • 초록

    느타리버섯균의 이핵체 체세포 균사에서 분열전 및 분열후의 핵과 분열중의 핵을 화학고정방법과 동결교체방법을 이용하여 균사분화과정중의 성상체 미세소관의 미세구조적 동태를 조사하였다. 많은 수의 성상체 미세소관들이 이동중인 분열전의 핵들과 연관되어 있었으며, 분열전의 핵이 이동하는 시기에 SPB의 분리가 진행됨이 발전되었다. 분열전 이동중의 핵과는 대조적으로 SPB의 분리가 더 이상 일어나지 않는 분열중의 이거나 분열이 끝난 핵에서는 성상체미세소관이 덜 발달되어 있었다. 활발히 이동할 것으로 추측되는 분열후의 핵에서 핵과 연관된 성상체미세소관이 잘 발달되어 있지 않음은 특이한 점이다. 분열중의 모든 핵에서 성상체미세소관이 SPB에서 뻗어나오며 인은 핵막에 남아있다. 핵의 분열과 핵의 이동 및 SPB의 분리와 관련된 성상체미세소관의 기능에 대하여 논의하였다.


    Premitotic, mitotic and postmitotic nuclei in the dikaryotic somatic hyphae of Pleurotus ostreatus, the oyster mushroom fungus were ultrastucturally examined using chemical fixation and freeze-substitution process, and the behaviors of astral microtubules associated with these nuclei were closely analyzed. Electron microscopic examinations revealed that astral microtubules are significantly abundant when the nuclei are in the stage of migration and at the stage of migration, the separation of spindle pole body occurs. Such an abundancy of astral microtubules in premitotic migrating nuclei is well contrasted with mitotic and postmitotic nuclei with much fewer astral microtubules and it should be noted that neither of these latter classes of nuclei exhibits the separation of the spindle pole body. It is remarkable that the postmitotic nuclei that are believed to migrate actively are associated with the astral microtubules that are less in numbers and length. During all the stages of nuclear division, astral microtubules are invariably radiating from the spindle pole bodies and nucleolus remains within the nuclear envelope of dividing nuclei throughout the division. The functions of astral microtubules developed during the nuclear division as well as the nuclear migration and separation of the spindle pole body were closely examined.


  • 주제어

    Astral microtubules .   Clamp .   Mitosis .   Nuclear migration .   Pleurotus .   Ultrastructure.  

  • 참고문헌 (31)

    1. Detection of spindle pushing forces in vivo during anaphase B in the fungus Nectria haematococca , Aist, J.R.;Bayles, C.J. , Cell Motility and the Cytoskel / v.19,pp.18-24,
    2. Ultrastructural basis of mitosis in the fungus Nectria haematococca. I. Asters , Aist, J.R.;Bayles, C.J. , Protoplasma / v.161,pp.111-122,
    3. Direct experimental evidence for the existence, structural basis and function of astral forces during anaphase B in vivo , Aist, J.R.;Bayles, C.J.;Tao, W.;Berns, M.W. , J. Cell Sci / v.100,pp.279-288,
    4. What makes microtubules dynamic? , Bayley, P.M. , J. Cell Sci / v.95,pp.329-334,
    5. Astral forces in fungal mitosis , Bayles, C.J.;Aist, J.R.;Tao, W.;Berns, M.W. , J. Cell Biol / v.107,pp.455a,
    6. Relationships between the movements of chloroplasts and cytoskeletons in diatoms , Chen, S.T.;Li, C.W. , Botanica Marina / v.34,pp.505-511,
    7. Quantitative analysis of sea urchin egg kinesin-driven microtubule motility , Cohn, S.A.;Ingold, A.L.;Sholey, J.M. , J. Biol. Chem. / v.264,pp.4290-4297,
    8. In vivo effects of orthovanadate on spindle structure and dynamics of locust spermatocytes. I. , Daub, A.M.;Hauser, M. , Chromosoma / v.93,pp.271-280,
    9. Cytoskeletal proteins and their genes in higher plants , Fosket, D.E. , Biochemistry of Plants / v.15,pp.393-454,
    10. Ultrastructure of mitosis in the cowpea rust fungus Uromyces phaseoli var , Heath, I.B.;Heath, M.C. , vignae. J. Cell Biol. / v.70,pp.592-607,
    11. Conformation and movement of the vegetative nucleus of the angiosperm pollen tube: association with the actin filament , Heslop-Harrison, J.;Heslop-Harrison, Y. , J. Cell Sci. / v.93,pp.299-308,
    12. Actomyosin and movement in the angiosperm pollen tube: an interpretation of some results , Heslop-Harrison, J.;Heslop-Harrison, Y. , Sex. Plant Repro / v.2,pp.199-207,
    13. Myosin associated with the surfaces of organelles, vegetative nuclei and generative cells in angiosperm pollen grains and tubes , Heslop-Harrison, J.;Heslop-Harrison, Y. , J. Cell Sci. / v.94,pp.319-325,
    14. Intracellular motility, the actin cytoskeleton and germinability in the pollen of wheat , Heslop-Harrison, J.;Heslop-Harrison, Y. , Sex. Plant Repro / v.5,pp.247-255,
    15. Cytoskeletal interactions with postmitotic migrating nuclei in the oyster mushroom fungus, Pleurotus ostreatus: evidence against a force generating role for astral microtubules , Kaminskyj, S.;Yoon, K.S.;Heath, I.B. , J. Cell Sci. / v.94,pp.603-674,
    16. Mechanismm of anaphase B movement;Biological functions of microtubules, and related structures , Kronebush, P.J.;Borisy, G.G.;Sakai, H.(ed.);Mohri, H.(ed.);Borisy, G.G.(ed.) , / v.,pp.233-245,
    17. The role of the cytoskeletal protein, tubulin, in the mode of action and mechanism of drug resistance to benzimidazole , Lacey, E. , Intern. J. Parasitology / v.18,pp.885-936,
    18. The arrangement of actin bundles and chloroplasts in the nodal regions of Characean internodal cells , McLean, B.;Juniper, B.E. , Europ. J. Phycol. / v.28,pp.33-37,
    19. The cytoskeleton of the giant coenocytic green alga Caulerpa visualized by immunocytochemistry , Menzel, D. , Protoplasma / v.139,pp.71-76,
    20. Fine structure of shitake, Lentinus edodes. VI. Cytoplasmic microtubules in relation to nuclear movement , Nakai, Y.;Ushiyama, R. , Can. J. Bot. / v.56,pp.1206-1211,
    21. The motor for poleward chromosome movement in anaphase is in or near the kinetochore , Nicklas, R.B. , J. Cell Biol. / v.109,pp.2245-2255,
    22. Mechanically cut mitotic spindles: clean cuts and stable microtubules , Nicklas, R.B.;Lee, G.M.;Rieder, C.L.;Rupp, G. , J. Cell Sci. / v.94,pp.415-423,
    23. Actin polymerization as an essential process in light- and dark-controlled chloroplast anchorage , Schonbohm, E.;Meyer-Wegner, J. , Biochem. Physiol. Pflanzen / v.185,pp.337-342,
    24. Analysis of spindle microtubule organization in untreated and taxol-treated PTK1 cells , Snyder, J.A.;Mullins, J.M. , Cell Biol. Intern. / v.17,pp.1075-1084,
    25. The translocation of mitochondria along insect ovarian microtubules from isolated nutritive tubes: a simple reactivated model , Stebbing, H.;Hunt, C. , J. Cell Sci. / v.88,pp.641-648,
    26. Patterns of nuclear migration and heterokaryosis in pairings between sibling homokaryons of Heterobasidion annosum , Stenlid, J.;Rayner, A.D.M. , Mycol. Res. / v.95,pp.1275-1283,
    27. Astral microtubules are not required for anaphase b in Saccharomyces cerevisiae , Sullivan, D.S.;Huffaker, T.C. , J. Cell Biol. / v.119,pp.379-388,
    28. The role of astral microtubules in conjugate division in the dikaryon of Coprinus cinereus , Tanabe, S.;Kamada, T. , Exp. Mycol. / v.18,pp.338-348,
    29. The role microtubules for the movement of mitochondria in pinacocytes of fresh water sponges (Spongillidae, Porifera) , Weissenfels, N.;Wachtman, D.;Stockem, W. , Europ. J. Cell Biol. / v.53,pp.310-314,
    30. Ultrastructure of mitosis and clamp formation in the somatic hyphae of Pleurotus ostreatus , Yoon, K.S.;Kim, Y.S. , Mycologia / v.86,pp.593-601,
    31. Basidiosporogenesis in Boletus rubinellus. II. Late spore development , Yoon, K.S.;McLaughlin, D.J. , Mycologia / v.78,pp.185-197,

 저자의 다른 논문

  • 윤권상 (10)

    1. 1990 "Staphylococcus aureus DH1에서 분리된 Macrolide-Lincosamide-Streptogramin B 계열 항생물질에 대한 저항성 인자의 특성과 염기서열" Korean journal of microbiology = 미생물학회지 28 (1): 27~34    
    2. 1992 "Trimorphomyces papilionaceous 에서 laccase 의 catabolite repression 에 의한 조절" Korean journal of microbiology = 미생물학회지 30 (2): 78~82    
    3. 1993 "담자성 효모 Trimorphomyces papilionaceus 이배체균의 세포학적 특성" Korean journal of microbiology = 미생물학회지 31 (1): 22~26    
    4. 1994 "Coprinus cinereus에서 Validamycin 저항성균주의 세포학적 변화" 한국균학회지 = The Korean journal of mycology 22 (1): 31~35    
    5. 1994 "액체배양한 Coprinus congregatus에서 세포막 연관 Iaccase의 생성 조절" 한국균학회지 = The Korean journal of mycology 22 (1): 46~49    
    6. 1996 "Fungal-Sporulation Suppressing Substances Produced by Pseudomonas aeruginosa KMCS-1" The journal of microbiology 34 (3): 284~288    
    7. 2000 "Pseudomonas aeruginosa에 의핸 생합성되는 향진균성물질(PAFS)의 생산성 증가 및 생산균주의 배양생리학적 특성 연구" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 28 (6): 341~348    
    8. 2004 "항진균물질을 생합성하는 Pseudomonas aeruginosa의 배양생리적 특성 연구" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology 32 (1): 52~59    
    9. 2005 "Morphological Characteristics of Conidiogenesis in Cordyceps militaris" Mycobiology 33 (2): 69~76    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기