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Effect of Ginseng Saponin on Gap Junction Channel Reconstituted with Connexin 32

Hong, Eun-Jung    (Department of Biochemistry, Yeungnam University   ); Huh, Keun    (Department of Pharmacology, Yeungnam University   ); Rhee, Seung-Keun    (Department of Biochemistry, Yeungnam University  );
  • 초록

    Panax-ginseng saponin has been known to exert various pharmacological effects on cellular metabolism. This study was performed to determine the effect of ginseng saponin on gap junction channel-mediated intercellular communication, using an established in vitro system of reconstituted gap junction channels. Gap junction channels are a specialized plasma membrane fraction, which are permeable to relatively large water-soluble molecules. The sucrose permeable property of reconstituted gap junction channels was completely inhibited with 0.1 % (w/v) of ginseng saponin. We also compared the effect of ginseng saponin with that of Triton X-100, a nonionic detergent, on the same system. Triton X-100 showed significantly different effect on sucrose-permeability of gap junction channel from that was affected by ginseng saponin. The structures of liposomes containing gap junction channels was significantly destroyed by Triton X-100.


  • 주제어

    Ginseng .   Gap junciton channel .   Connexin32 .   Sucrose-permeability .   Tiriton X-100 .   Reconstitution.  

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  • 허근 (57)

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    10. 1987 "Effect of Carbon Tetrachloride Intoxication on the Type Conversion of Xanthine Dehydrogenase Into Xanthine Oxidase in Rats" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 10 (1): 36~41    
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