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인조혈관재료 표면에 도포된 혈관내피세포의 생리적 변화에 관한 연구
Physiological Function of Endothelial Cells Cultured on Polyurethsne Coated by ECM

이윤신    (서울의대 의공학 연구소   ); 김용배    (순천향 대학병원 성형외과, 부천세종병원 내과  );
  • 초록

    Antithrombogenic surFace is one of the most important things to the artificial vascular prostheses. This problem will be solved if the surface of prosthesis is covered with endothelial cells. The attachment and the growth of endothelial cells onto vascular prosthesis are very difficult. So many studies have been concentrated on the attachement of endothelial cell. But no good performance of the in uiwo experiments has been shown until now. In this study, we used the whole extracellular matrix (ECM) excreted from fibroblasts as an underlying matrix, and the endothelial cells were seeded to obtain the long term patency of vascular graft(i.e., for the patent 8 week implanted wafts in the animal model of rat). In order to study the antithrombogenic functions of cultured endothelial cells, prostaglandin(PGF 1 a) synthesis and platelet adhesion were assayed. The concentration of PGF a of stimulated group was sisnificantly higher than that of control group(21.97 $\pm$ 3.45 vs 4.93 $\pm$ 0.71 pg/1000 cells). The platelet adhesion of the polyurethane sheet covered with endothelial cells was lower than that of polyurethane sheet or sheet covered with ECM(1.04 $\pm$ 0.28, 2.87 $\pm$ 0.77, 2.89 $\pm$ 0.70, % radioactivities, respectively). Endothelial cells grew well on polyurethane coated with ECM, synthesized the prostacyclin and functioned well as antithrombogenic. Therefore the endothelialization onto the ECM excreted from fibroblasts may be a good method for the vfudig prosthesis.


  • 주제어

    Endothelialization .   Antithrombogenic function .   Vascular Prosthesis .   Extracellular matrix.  

  • 참고문헌 (21)

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