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The journal of microbiology v.34 no.1, 1996년, pp.49 - 53   피인용횟수: 2

Effects of $K^+$ lon on in vitro RNA Splicing of T4 Phage Thymidylate Synthase Gene

Sung, Jung-Suk    (Department of Applied Biology, Dongguk University ;  ); Park, In-Kook  
  • 초록

    The effects of K $^{+}$ ion on the activity of RNA splicing of T4 phage thymidylate synthase gene have been investigated. The splicing activity was stimulated within the range of 5 to 20 mM concentration of KCI. When the concentration of KCI in the splicing reaction was brought to 100 or 200 mM a small amount of the exonl-intron product (1, 4 kb) was formed with large proportion of primary RNA transcript not undergoing splicing. This observation strongly suggests that there may exist come kinds of interferences with transesterification at the first step of splicing. Overall it can be concluded that K $^{+}$ ion exhibits very unique roles in RNA splicing of tdd gene depending on its concentration.ion.


  • 주제어

    T4 phage td intron .   K ion .   RNA splicing.  

  • 참고문헌 (17)

    1. Role of conserved sequence elements 9L and 2 in self-splicing of Tetrahymena ribosomal RNA precursor , Burke, J.M.;K.D. Irvine;K.L. Kaneko;B.J. Kerker;A.B. Oettger;W.M. Tierney;C.L. Williamson;A.J. Zaug;T.R. Cech , Cell / v.45,pp.167-176,
    2. Effect of monovalent cations and spermidine on RNA splicing of T4 phage td intron , Park, I.K.;J.S. Sung;S. Shin , Proceedings of the 7th FAOBMB Congress / v.,pp.,
    3. Metal ion catalysis in the Tetrahymena ribozyme reaction , Piccirilli, J.A.;J.S. Vyle;M.H. Caruthers;T.R. Cech , Nature / v.361,pp.85-88,
    4. Self-cleavage of RNA in the replication of small pathogens of plants and animals , Symons, R.H. , Trends in Biochem. Sci. / v.14,pp.445-450,
    5. Reaction conditions and kinetics of self-cleavage of a ribozyme derived from Neurospora VS RNA , Colins, R.A.;K.E. Olive , Biochemistry / v.32,pp.2795-2799,
    6. The chemistry of self-splicing RNA and RNA enzyme , Cech, T.R. , Science / v.236,pp.1532-1539,
    7. Moore, Walter J. , Physical Chemistry / v.,pp.420-457,
    8. A model for splicing in fungal mitochondria , Davies, R.W.;R.B. Waring;R.A. Ray;T.A. Brown;C. Scazzocchio , Nature / v.300,pp.719-724,
    9. Metal ion requirements for sequence-specific endonuclease activity of the Tetrahymena ribozyme , Grosshans, C.A.;T.R. Cech , Biochemistry / v.28,pp.6888-6894,
    10. Role of $Mg^{2+}$ in RNA splicing of T4 td intron , Sung, J.S.;S. Shin;I.K. Park , J. Microbiol. / v.33,pp.160-164,
         
    11. Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of RNase P from E. coli , Guerrier-Takada, C.;K. Haydock;L. Allen;S. Altman , Biochemistry / v.25,pp.1509-1515,
    12. The rate and specificity of a group Ⅰribozyme are inversely affected by choice of monovalent salts , Partona, S.;A.S. Lewin , Nucl. Acid. Res. / v.19,pp.605-609,
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    14. Effects of $Mn^{2+}$ and $Zn^{2+}$ on in vitro RNA splicing of T4 phage thymidylate synthas gene , Sung, J.S.;S. Shin;I.K. Park , Dongguk Univ. J. Natural Science / v.,pp.,
    15. The guanosine binding site of the Tetrahymena ribozyme , Michel, F.;M. Hanna;R. Green;D.P. Bartel;J.W. Szostak , Nature / v.342,pp.391-395,
    16. In vitro splicing of the ribosomal RNA precursor of Tetrahymena , Cech, T.R.;A.J. Zaug;P.Z. Grabowski , Cell / v.27,pp.487-496,
    17. RNA splicing: three themes with variation , Cech, T.R. , Cell / v.34,pp.713-716,
  • 이 논문을 인용한 문헌 (2)

    1. Park, In-Kook ; Sung, Jung-Suk ; Shin, Sook 1997. "" Korean journal of biological sciences, 1(1): 87~91     
    2. 2002. "Effects of Divalent Cations on the Self-splicing Inhibition of Group I Intron by the Coen-zyme Thiamine Pyrophosphate" Korean journal of microbiology = 미생물학회지, 38(1): 13~18     

 저자의 다른 논문

  • Sung, Jung-Suk (14)

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    2. 1995 "Role of $Mg^{2+}$ in RNA splicing of T4 td intron" The journal of microbiology 33 (2): 160~164    
    3. 2005 "Formation of DNA-protein Cross-links Mediated by C1'-oxidized Abasic Lesion in Mouse Embryonic Fibroblast Cell-free Extracts" Integrative biosciences 9 (2): 79~85    
    4. 2006 "생체의료용 기능성 고분자 재료의 개발" 폴리머 = Polymer (Korea) 30 (1): 1~9    
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