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The journal of microbiology v.34 no.1, 1996년, pp.82 - 89   피인용횟수: 3

Purification and Characterization of Purine Nucleoside Phosphorylase (PNP) in Micrococcus luteus

Choi, Hey-Seon  
  • 초록

    Purine nucleoside phosphorylase (PNP) was purified in Micrococcus luteus (M. luteus) using streptomycin sulfate and amomonium sulfate fractionation, three times by a Sephadex G-100 gel filtration and a DEAE-Sephadex A-50 ion exchange chromatography. The enzyme was purified 72 folds with a 11% recovery and showed a single band in a nondenaturing gel electrophoresis. The M. W. of PNP turned out to be 1.35 * 10 $^{5}$ delton in G-150 gel filtration chromatography. The stability of the enzyme was increased by treatment with both substrates, MgCI $_{2}$ or CaCI $_{2}$ , but not significantly kcal/mol. M. luteus PNP catalyzed the phosphorolysis of inosine, deoxyinosine, guanosine and deoxyguanosine with the Km value of 1.5 * 10 $^{-3}$ M, 3.0 * 10 $^{-3}$ M, 5.0 * 10 $^{-4}$ M, respectively. The enzyme was reacted with adenosine, 1-methylnosine and 1-methylguanosine as substrates, which were shown to be poor substrates for mammalian enzyme.


  • 주제어

    Purine nucleoside phosphorylase .   Micriococcus luteus .   inosine .   guanosine .   adenosine .   1-me-thylinosine .   purification.  

  • 참고문헌 (37)

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  • 이 논문을 인용한 문헌 (3)

    1. 1996. "" The journal of microbiology, 34(3): 270~273     
    2. 1997. "" The journal of microbiology, 35(1): 15~20     
    3. 2000. "Purification and Properties of Serratia marcescens Purine Nucleoside Phosphorylase." 산업미생물학회지 = Korean journal of applied microbiology and biotechnology, 28(5): 251~257     

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