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The journal of microbiology v.34 no.1, 1996년, pp.90 - 94   피인용횟수: 2

Purification of carbosymethyl cellulase from hybrid between aspergillus niger and penicillium verruculosum

Yang, Young-Ki    (Department of Genetic Engineering, Chosun University   ); Lee, Jung-Sup    (Department of Genetic Engineering, Chosun University   ); Park, Hyung-Nam    (Department of Genetic Engineering, Chosun University   ); Moon, Myung-Nim    (Department of Genetic Engineering, Chosun University   ); Kim, Hong-Sub    (Department of Biology Chosun University   ); Kim, Jong-Se    (Department of Biology Chosun University   ); Lim, Chae-Young    (Department of Genetic Engineering, Chosun University ;  ); Rhee, Young-Ha  
  • 초록

    The carboxymethyl cellulase (CMCase) was purified from the induced culture filtrate of hybrid TAPW15703 between Aspergillus niger and penicillium verruculosum made by nuclear transfer. The enzyme was purified 80 fold with an overall yield 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and DEAE-ion exchange column chromatography. The molecular weight of the CMCase has estimated to be 32,000 daltons on SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel permeation chromatography. The purified enzyme functions optimally at pH 4.0 and 4 $0^{\circ}C$ The Km value for carbosymethyl cellulose was 68 mM. The enzyme activity was increased by the presence of $Mg^{2+}$ and Mn $^{2+}$ .


  • 주제어

    Asprergillus niger .   Penicillium verruculosum .   TAPWj 157-3 carboxymethyl cellulase .   purification.  

  • 참고문헌 (24)

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  • 이 논문을 인용한 문헌 (2)

    1. Lusta, Konstantin A. ; Chung, Il-Kyung ; Sul, Ill-Whan ; Park, Hee-Sung ; Shin, Dong-Ill 1999. "Characterization of an Extracellular Cellulose-Hydrolyzing Enzyme Complex from a Thermotolerant Strain of Aspergillus sp." Journal of microbiology and biotechnology, 9(6): 873~876     
    2. 2003. "Isolation and Characterization of a Novel Aspergillus tubingensis with a Hydrolyzing Activity of Cellulose-pectin Complex" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 31(2): 124~128     

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