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The journal of microbiology v.34 no.2, 1996년, pp.158 - 165  

Genetic Relationship between the SPT3 Gene and RAS/cAMP Pathway in Yeast Cell Cycle Control
Genetic relationship between the SPT3 gene and ARS/cAMP pathway in yeast cell cycle control

Shin, Deug-Yong    (Korea Research Institute of Bioscience and Biotechnology ;  ); Yun, Jean-Ho  
  • 초록

    The signal transduction pathways through the RAS gene product and adenyl cyclease play a critical role in regulation of the cell cycle in yeast, Saccharomyces cerevisiae. We examined the genetic relationship between the spt3 gene and ras/cAMP pathway. A mutation in the SPT3 gene suppressed cell cycle arrest at the G1 phase caused by either an inactivation of the RAS or CYR1 gene which encodes a yeast homologue of human ras proto-oncogene or adenyl cyclase, respectively. The phenotypes such as sporulation and heat shock resistancy, that resulted from a partial inactivation of the RAS or CYR1 genes, were also suppressed by the spt3 mutation. Expression of the SSA1 gene encoding one of th heat shock proteins (Hsp70) can be induced by heat shock or nitrogen starvation. Expression of this gene is derepressed in cry1-2 and spt3 mutants. The bcy 1 mutation repressed by the bcy1 mutation, but not in spt3 mutants. These results suggest that the SPT gene is involved in expression of genes that are affected by the RAS/cAMP pathway.


  • 주제어

    Cell dycle .   RAS/cAMP pathway SPT3.  

  • 참고문헌 (31)

    1. Differential regulation of the 70K heat shock gene and related genes in Saccharomyces cerevisiae , Ellwood, M.S.;E.A. Craig , Mol. Cell Biol. / v.4,pp.1454-1459,
    2. Genetic control of cell division cycle in yesat; a model , Hartwell, L.H.;J. Cloutti;J.R. Pringle;B.J. Reid , Science / v.183,pp.46-51,
    3. Control of cell division in Saccharomyces cerevisiae mutants defective in adentlate cyclase and cAMP-dependent protein kinase , Matsumoto, K.;I. Uno;T. Ishikawa , Exp. Cell. Res. / v.146,pp.151-161,
    4. The SPT3 gene is equired for normal tanscription of Ty element in Saccharomyces cerevisiae , Winston, F.;K.J. Durbin;G.R. Fink , Cell / v.39,pp.675-685,
    5. Pringle, J.R.;L.H. Hartwell;Strathern J.N.(ed.);E.W. Jones(ed.);J.R. Broach(ed.) , Molecular Biology of Yesat; life cycle and inheritance / v.,pp.,
    6. The isolation and genetic analysis of sporulation deficient mutants in Saccharomyces cerevisiae , Tsuboi, M , Mol. Gen. Genet. / v.191,pp.17-21,
    7. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerewisiae , Guarente, L.;M. Ptashne , Proc. Natl. Acad. Sci. / v.78,pp.2199-2203,
    8. Characterization of a cyclic nucleotide phosphodiestease-deficient mutant in yeast , Uno, I.;K. Maatsumoto;T. Ishikawa , J. Biol. chem. / v.258,pp.3539-3545,
    9. Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast , Uno, I.;K. Matsumoto;K. Adachi;T. Ishijkawa , J. Biol. Chem. / v.258,pp.10867-10872,
    10. Analysis of the yeast SPT3 gene and identification of its products, a positive regulator of Ty transcription , Winston, F.;P.L. Minehart , Nucleic Acids Res. / v.14,pp.6885-6900,
    11. Evidence for transposition of dispersed repetitive DNA families in yeast , Cameron, R.R.;E.Y. Loh;R.W. Davis , Cell / v.16,pp.739-751,
    12. Laboratory course mannul for methods in yesat genetics , Sherman, F.;G.R. Fink;J.B. Hicks , Yeast Genetics / v.,pp.,
    13. Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila , Ingolia, T.D;M.R. Slater;E.A. Craig , Mol. Cell. Biol. / v.2,pp.1388-1398,
    14. Heat shock response of Saccharomyces cerevisiae altered in cyclic AMP-dependent protein phosphorylation , Shin, D.Y.;K. Matsumoto;H. Iida;I. Uno;T. Ishikawa , Mol. Cell. Biol. / v.7,pp.244-250,
    15. Positive and negative regulation of basal expression of a yeast HSP70 gene , Park, H.O.;E.A. Craig , Mol. Cell. Biol. / v.9,pp.2025-2033,
    16. Initiation of meiosis in yeast mutants defective in adenylate cyclase and protein kinase , Matsumoto, K.;I. Uno;t. Ishikawa , Cell / v.31,pp.417-428,
    17. Genes controlling meiosis and spore formation in yeast , Esposito, M.;R.E. Esposito , Genetics / v.78,pp.215-225,
    18. Regulation of mating and meiosis in yesat by mating type locus , Kassir, T.;G. Simchen , Genetics / v.82,pp.187-206,
    19. Manniatis, T.;E.F. Fritsch;J. Sambrook , Molecular Cloning; A Laboratory Manuual / v.,pp.,
    20. Mutation affecting Ty-mediated expression of the HIS4 gene of Saccharamyces cerevisiae , Winston, F.;D.T. Chaleff;B. Valent;G.R. Fink , Genetics / v.107,pp.179-197,
    21. Specific early G1 block accompained with stringent response in Saccharomyces cerevisiae , Iida, H.;I. Yahara , Mol. Cell. Biol. / v.8,pp.822-827,
    22. Three different genes in Saccharomyces cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase , Toda, T.;S. Cameron;P. Sass;M. Zoller;M. Wigler , Cell / v.50,pp.277-287,
    23. Miller, J.H , Experiments in Molecular Genetics / v.,pp.,
    24. Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase , Matsumoto, K.;I. Uno;Y. Oshima;T. Ishikawa , Proc. Natl. Acad. Sci. / v.79,pp.2355-2359,
    25. Transformation of intact yeast cells treated with alkali cations , Ito, H.;Y. Fukuda;K. Murata;A. Kimura , J. Bacteriol. / v.153,pp.163-168,
    26. Control of the G1/Go transition and Go protein synthesis in Saccharomyces cerevisiae , Shin, D.Y.;I. Uno;T. Ishikawa , Curr. Genet. / v.12,pp.577-585,
    27. Genetic analysis of yeast RAS1 and RAS2 genes , Kadaoka, T.;S. Power;C. McGill;O. Fasano;J. Strathern;J. Broach;M. Wigler , Cell / v.37,pp.437-445,
    28. DNA sequence and characterization of the Saccharomyes cerevisiae gene encoding adenylate cyclase , Kadaoka, T.;D. Broke;M. Wigler , Cell / v.43,pp.493-505,
    29. SPT3 interact with THIID to allow normal transcription in Saccharomyces cerevisiae , Eisenman, D.M.;K.M. Arndt;S.L. Ricupero;J.W. Rooney;F. Winston , Genes & Devel. / v.6,pp.1319-1331,
    30. Isolation and characterization of temperature-sensitive mutations in the RAS2 and CYR1 genes in Saccharomyces cerevisiae , Mistuzawa, H.;I. Uno;T. Oshima;T. Ishikawa , Genetics / v.123,pp.739-748,
    31. Mutation affecting sexual conjugation and related processes in Saccharomyces cerevisiae , MacKay, V.I.;T.R. Manney , Genetics / v.76,pp.272-288,

 저자의 다른 논문

  • Shin, Deug-Yong (1)

    1. 1997 "Novel strategy for isolating suppressors of meiosis-deficient mutants and its application for isolating the bcy1 suppressor" The journal of microbiology 35 (1): 61~65    

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