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The journal of microbiology v.34 no.2, 1996년, pp.179 - 183  

Optimization of Culture Conditions for Production of Pneumococcal Capsular Polysaccharide Type I

Kim, Su-Nam    (Department of Pharmacy, SungKyunKwan University   ); Min, Kwan-Ki    (Department of Pharmacy, SungKyunKwan University   ); Kim, Seung-Hwan    (Department of Pharmacy, SungKyunKwan University   ); Choi, In-Hwa    (Department of Pharmacy, SungKyunKwan University   ); Lee, Suhk-Hyung    (Department of Pharmacy, SungKyunKwan University   ); Pyo, Suhk-Noung    (Department of Pharmacy, SungKyunKwan University   ); Rhee, Dong-Kwon    (Department of Pharmacy, SungKyunKwan University  );
  • 초록

    Streptoccus Pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide (PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsulard PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was uwed to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not ehnahce growth or PS production. Substitution of netrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium ion) had adverse affects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/1 concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synerigistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cummulative increase in capsular PS production.


  • 주제어

    Streptococcus pnemoniae type 1 .   polysaccharide .   culture opticization.  

  • 참고문헌 (11)

    1. Oligosaccharides , White, C.A.;J.F. Kennedy;M.F. Chaplin(ed.);J.F. Kennedy(ed.) , Carbohydrate analysis: a practical approach / v.,pp.37-38,
    2. Characterization of some pneumococcal bacteriophage , Porte, R.D.;W.R. Guild , J. Virol. / v.19,pp.659-667,
    3. A new synthetic medium for Diplococcus pneumoniae, and its use for the sutdy of reciprocal transformations at the amiA locus , Sicard, A.M , Genetics / v.50,pp.31-44,
    4. Serotypes of Streptococcus pneumoniae isolated from blood cultures at Boston city hospital between 1979 and 1982 , Barry, M.A.;D.E. Craven;M. Finland , J. Infec. Diseases. / v.149,pp.449-452,
    5. Steptococcus pneumoniae , Willett, H.P.;Joklik, W.K.(ed.);H.P. Willett(ed.);D.B. AmosA(ed.);C.M. Wilfert(ed.) , Zinsser Mcirobiology / v.,pp.368-377,
    6. The pneumococcal polysaccharides: a re-examination , Larm, O.;B. Lindberg , Adv. Carbohydrate Chem. & Biochem. / v.33,pp.295-322,
    7. The pneumococcus and bacterial pneumonia , Storch, G.;M. Schaechter(ed.);G. Medoff(ed.);D. Schlessinger(ed.) , Mechanism of microbial disease / v.,pp.218-227,
    8. Bacterial Polysaccharides , Kenne, L.;B. Lindberg;G.O. Aspinall(ed.) , The Polysaccharides / v.2,pp.287-352,
    9. Quantatitive studies of the spectificity of anti-pneumococcal polysaccharide antibodies, type Ⅲ and Ⅷ , Campbell, J.H.;A.M. Pappenheimer , Immunochem. / v.3,pp.195-212,
    10. Coligan, J.E.;A.M. Krusbeek;D.H. margulies;E.M. Sshevach;W. Strober(eds.) , Current Protocols in Immunology / v.,pp.2.3.1-2.3.4,
    11. A partially defined medium for cultivation of pneumococcus , Adams, M.H.;A.S. Roe , J. Bacteriol. / v.49,pp.401-409,

 저자의 다른 논문

  • Min, Kwan-Ki (1)

    1. 1999 "폐렴구균 Type 23 협막다당류 생산의 최적화" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 7 (3): 257~262    
  • Kim, Seung-Whan (4)

  • Choi, In-Hwa (1)

  • Pyo, Suhk-Neung (61)

  • Rhee, Dong-Kwon (51)

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