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The journal of microbiology v.34 no.2, 1996년, pp.198 - 205  

Partial Characterization of Proteases from Culture Filtrate of Mycobacterium tuberculosis

Na, Byoung-Kuk    (Department of Biology, Faculty of Natural Science, Chung-Ang University   ); Song, Chul-Yong    (Department of Biology, Faculty of Natural Science, Chung-Ang University   ); Park, Young-Kill    (Korea Institute of Tuberculosis   ); Bai, Gill-Han    (Korea Institute of Tuberculosis   ); Ki, Sang-Jae    (Korea Institute of Tuberculosis  );
  • 초록

    Two proteases were partially characterized from culture filtrate of Mycobacterium, tuberculosis KIT110. Their molecular weights were approximately 200 and 180 kDa, respectively and they exhibited similar enzymatic characteristics. These enzymes were inhibited significantly by EDTA and to some extent by EGTA. Their activity was enhanced by $Ca^{2+}$ and $Mg^{2+}$ to some degree. However, $Cu^{2+}$ and $Ag^{2+}$ completely inhibited the enzyme activity at the concentration of 2.5 and 5 mM, respectively. The optimal pH was 7.0 and optimal temperature was around $40^{\circ}C$ . These enzymes were rapidly inactivated at $80^{\circ}C$ . Therefore, they were heat-labile, neutral metalloproteases. These enzymes exhibited antigenicity shown by their reacting with sera from the partients with pulmonary tuberculosis. These enzymes were able to degrade serum proteins including hemoglobin, bovine serum albumin, lysozyme and immunoglobulin G and structural matrix protein such as type I collagen. Therefore, these enzymes may be thought to contribute to tissue necrosis and pathogenesis during infection.

  • 주제어

    Mycobacterium tuberculosis .   characterization .   proteases.  

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