본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The journal of microbiology v.34 no.2, 1996년, pp.198 - 205  

Partial Characterization of Proteases from Culture Filtrate of Mycobacterium tuberculosis

Na, Byoung-Kuk    (Department of Biology, Faculty of Natural Science, Chung-Ang University   ); Song, Chul-Yong    (Department of Biology, Faculty of Natural Science, Chung-Ang University   ); Park, Young-Kill    (Korea Institute of Tuberculosis   ); Bai, Gill-Han    (Korea Institute of Tuberculosis   ); Ki, Sang-Jae    (Korea Institute of Tuberculosis  );
  • 초록

    Two proteases were partially characterized from culture filtrate of Mycobacterium, tuberculosis KIT110. Their molecular weights were approximately 200 and 180 kDa, respectively and they exhibited similar enzymatic characteristics. These enzymes were inhibited significantly by EDTA and to some extent by EGTA. Their activity was enhanced by $Ca^{2+}$ and $Mg^{2+}$ to some degree. However, $Cu^{2+}$ and $Ag^{2+}$ completely inhibited the enzyme activity at the concentration of 2.5 and 5 mM, respectively. The optimal pH was 7.0 and optimal temperature was around $40^{\circ}C$ . These enzymes were rapidly inactivated at $80^{\circ}C$ . Therefore, they were heat-labile, neutral metalloproteases. These enzymes exhibited antigenicity shown by their reacting with sera from the partients with pulmonary tuberculosis. These enzymes were able to degrade serum proteins including hemoglobin, bovine serum albumin, lysozyme and immunoglobulin G and structural matrix protein such as type I collagen. Therefore, these enzymes may be thought to contribute to tissue necrosis and pathogenesis during infection.


  • 주제어

    Mycobacterium tuberculosis .   characterization .   proteases.  

  • 참고문헌 (32)

    1. Crystalline soybean trypsin inhibitor , Kunitz, M , J. Gen. Physiol. / v.30,pp.291-310,
    2. Purification and characterization of four proteeases from a clinical isolate of Serratia marcescens kims 3958 , Matsumoto, K.;H. Maeda;K. Takata;R. Kamata;R. Okamura , J. Bacteriol. / v.157,pp.225-232,
    3. Tcell response to Mycobacterium tuberculosis , Orme, I.M.;P.A. Andersen;W.H. Boom , J. Infect. Dis. / v.167,pp.1481-1497,
    4. Antigens in culture supernatant of Mycobacerium tuberculoss : epitopes defined by monoclonal and human antibodies , Verbon, A.;S. Kuuper;H.M. Jansen;P. Speelman;A.H.J. Kolk , J. Gen. Microbiol. / v.136,pp.955-964,
    5. Isolation and partial cahracterization of major protein antigens in the culture fluid of Mycobacterium tuberculosis , Nagai, S.;H.G. Wiker;M. Harboe;M. Kinomoto , Infect. Immun. / v.59,pp.372-382,
    6. Virulence factors of the family Legionellacea , Dowling, J.N.;A.K. Saha;R.H. Glew , Micorbiol. Rev. / v.56,pp.32-60,
    7. Protein measurement with the foline phenol reagent , Lowrhy, O.H.;N.J. Rosebrugh;A.L. Farr;R.J. Randall , J. Biol. Chem. / v.193,pp.265-275,
    8. The antigen 85 complex : a secretion product of Mycobacterium tuberculosis , Wiker, H.G.;M. Harboe , Microbiol. Rev. / v.56,pp.648-663,
    9. Attachment of mycobacteria to fibronectincoated surfaces , Ratliff, T.L.;J.A. McGarr;C. Abou-Zeid;G.A.W. Rook;J.L. Standford;J. Aslanzadeh;E.J. Brown , J. Gen. Microbiol. / v.134,pp.1307-1313,
    10. Partial characterization of the proteolytic seretions of Acanthamoeba polyphaga , Mitro, K.;A. O-M. Bhagaratathiammai;G. Zhou;J.H. Bobett;J.H. McKerrow;R. Chokshi;E.R. James , Exp. Parasitol. / v.78,pp.377-385,
    11. Properties of proteins MPB64, MPB70 and MPB80 of Mycobacterium bovis BCG , Harboe, M.;S. Nagai;M.E. Patarroyo;M.L. Torres;C. Ramirez;N. Cruz , Infect. Immun. / v.52,pp.293-302,
    12. Cleavage of structural protein during the assembly of the head of bacteriophage T4 , Laemmli, D.K , Nature / v.227,pp.680-685,
    13. Biological activity of protein antigens isolated from Mycobacterium tuberculosis culture filtrate , Collins, F.M.;J.R. Lamb;D.B. Young , Infect. Immun. / v.56,pp.1260-1266,
    14. Electrophoretic analysis of lasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substances , Heussen, C.;E.B. Dowdle , Anal. Biochem. / v.102,pp.196-202,
    15. IgG proteolytic activity of Pseudomonas aeruginosa in cystic fibrosis , Fick, R.B.;R.S. Baltimore;S.U. Suier;H.Y. Reynolds , J. Infect. Dis. / v.151,pp.589-597,
    16. Pathogenic potenials of bacterial protease , Maeda, H.;A. Molla , Clin. Chem. Acta. / v.185,pp.357-368,
    17. A localization index for distinction between extracellular and intracellular antigens of Mycobacterium tuberculosis , Wiker, G.H.;M. Harboe;S. Nagai , J. Gen. Microbiol. / v.137,pp.875-884,
    18. The secreted antigens of Mycobacterium tuberculosis and their relationship to those recognized by the available antibodies , Abou-Zedid, C.;I. Smith;J.M. Grange;T.L. Ratliff;J. Steele;G.A.W. Rook , J. Gen. Microbiol. / v.134,pp.531-538,
    19. Proteins released from Mycobacterium tuberculosis during growth , Andersen, P.;D. Askgaard;L. Ljungavist;J. Bennedsen;I. Heron , Infect. Immun. / v.59,pp.1905-1910,
    20. Characterization of fibronectin-binding angigens released by Mycobacterium tuberculosis and Mycobacterium bovis BCG , Abou-Zeid, C.;T.L. Ratliff;H.G. Wiker;M. harboe;J. Bennedsen;G.A.W. Rook , Infect. Immun. / v.56,pp.3046-3051,
    21. Tuberculosis in developing cuntries : burden, inervention and cost , Murray, C.J.L.;K. Styblo;a. Rouillon , Tuberd. Lung Dis. / v.65,pp.6-24,
    22. MPB59, a aidly cross-reacting protein of Mycobacterium bavis BCG , Wiker, H.G.;M. Harboe;S. Nagai;M.E. Patarroyo;C. Ramirez;N. Cruz , Int. Arch. Allergy Appl. Immun. / v.81,pp.307-314,
    23. Effective vaccination of mice against Mycobacterium tuberculosis infection wuth a solube mixture of secreted mycobacterial proteins , Andersen, P , Infect. Immun. / v.62,pp.2536-2544,
    24. Induction of non-specific acquired rsistance and delayed-type hypersensitivity, but not specific acqurid resistnce, in mice inoculated with killed myhcobacterial vaccines , Orme, I.M , Infect. Immun. / v.56,pp.3310-3312,
    25. Isolation and general characterization of a heat stable proteinase form Pseduomonas fluorescens AFT 36 , Stepaniak, L.;P.F. Fox;C. Daly , Biochim. Biophys. Acta. / v.717,pp.376-383,
    26. Specific cleavage of human type Ⅲ and Ⅳ collagnes by Pseudomonas aeruginosa elastase , Heck, L.W.;K. Morihara;W.B. McRAE;E.J. Miller , Infect. Immun. / v.51,pp.115-118,
    27. Intravesical bacillus Calmette-Guerin theraphy for murine bladder tumors : initiation of the response byh fibronectin-mediated attachment of bacillus Calmette-Guerin , Ratliff, T.L.;J.O. Palmer;J. McGarr;E.J. Brown , Cancer Res. / v.47,pp.1762-1766,
    28. MPB70, a unique antigen of Mycobacterium bovis BCG , Harboe, M.;S. Nagai , Am. Rev. Respir. Dis. / v.129,pp.444-452,
    29. Purification and characterization of two protein antigens from the heterogenous BCG 85 complex in Mycobacterium bovis BCG , Wiker, H.G.;M. Haboe;T.E. Lea , Int. Arch. Allergy Appl. Immun. / v.81,pp.298-306,
    30. Mycobacterial protein antigens : a complication , Young, D.B.;S.H. Kaufmann;P.W.M. Hermans;J.E.R. Thole , Mol. Microbiol. / v.6,pp.133-145,
    31. The biology of oxygen radicals , Fridovic, I , Science / v.201,pp.875-880,
    32. T-cell proliferiative response to antigens secreted by mycobacterium tuberculosis , Andersen, P.;D. Askgaard;L. Ljungavist;M.W. Beneden;I. Heron , Infect Immun. / v.59,pp.1558-1563,

 저자의 다른 논문

  • 송철용 (19)

    1. 1982 "담수어중(淡氷魚中)의 총수은함량(總水鎭含量)에 관(關)한 연구(硏究)" 한국식품과학회지 = Korean journal of food science and technology 14 (2): 168~173    
    2. 1985 "한국의 요꼬가와흡충(Metagonimys yokogawai)에 관한 연구 IV. 동남부 지방산 은어의 지리적 분포 및 요꼬가와흡충 피낭유충의 감염상태" 기생충학잡지 = The Korean journal of parasitology 23 (1): 123~139    
    3. 1991 "Pseudomonas aeruginosa 세포질외 serine계열 단백질 분해효소의 정제 및 특성" Korean journal of microbiology = 미생물학회지 29 (6): 345~352    
    4. 1992 "스파르가눔의 cysteine proteinase에 대한 단세포군 항체의 제조 및 특성 분석" 한국생화학회지 25 (3): 262~268    
    5. 1992 "Spirometra Plerocerocoid (sparganum) 의 분비 배설물에서 Cysteine Proteinase 정제 및 부분 특성" 한국생화학회지 25 (3): 276~282    
    6. 1995 "Purification and characterization of an extracellular protease from culture filtrate of salmonella schttmulleri" The journal of microbiology 33 (3): 244~251    
    7. 1996 "General Primer를 이용한 무균성뇌막염 원인 바이러스 분석" 대한바이러스학회지 = The journal of the Korean society of virology 26 (2): 215~225    
    8. 1996 "Purification and Characterization of a Serine Proteinase from Acanthamoeba culbertsoni" Journal of biochemistry and molecular biology = 한국생화학회지 29 (5): 455~461    
    9. 1997 "Purification and Characterization of Extracellular Aspartic Proteinase of Candida albicans" The journal of microbiology 35 (2): 109~116    
    10. 2000 "Intracellular Posttranslational Modification of Aspartyl Proteinase of Candida albicans and the Role of the Glycan Region of the Enzyme" The journal of microbiology 38 (4): 218~223    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기