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The journal of microbiology v.34 no.3, 1996년, pp.236 - 240  

Detection of Rifampin Resistance Mutation and Its Altered Nucleotide Sequences in Mycobacterium leprae Isolated from Korean Patients with Leprosy

Kim, Soon-Ok    (Myong Ji University   ); Kim, Min-Joo    (Catholic University Medical College   ); Tae, Chae-Gue    (Catholic University Medical College ;  ); Suh, Joo-Won  
  • 초록

    Rifampin is the most powerful drug for treating leprosy and tuberculosis today. It inhibits initiation and elongation of RNA transcription by binding to $\beta$ -subunit of RNA polymerase, leading to kill mycobacteria. We isolated one variant strain of Mycobacterium leprae from 24 Korean leprosy patients who are less susceptible to rifampin or have suffered from relapse by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) of the rpoB gene. Direct sequencing of the rpoB region of M. leprae variant revealed missense mutations which altered the amino acids sequenceof RpoB to Ser-464, Arg-465, Arg-467 and Ala-468. This is the first finding on rpoB gene mutation of M. leprae from Korean patients ; moreover the mutant type was found to be different from the previously reported cases in other countries.


  • 주제어

    Mycovacterium leprae .   PCR-SSCP .   direct sequcecing .   multivacilary leprosy .   missense mutatuion.  

  • 참고문헌 (17)

    1. Detection of Mycobacterium leprae and the potential for monitoring antileprosy drug therapy directly from skin biopsies by PCR , Williams, D.L. , Molecular and Cellular Probes / v.6,pp.401-410,
    2. A non-radioactive method for simultaneous detection of single-strand conformation polymorphisms (SSCP) and heteroduplexes , Lee, S.T.;S.K. Park;K.H. Lee;S.A. Holmes;R.A. Spritz , Mol. Cells / v.5,pp.668-672,
    3. A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae , Honore, N.;E. Perrani;A. Telenti;J. Grosset;S.T. Cole , Int. J. Lep. / v.61,pp.600-604,
    4. Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP , Sakar, G.;Yoon, H.S.;S.S. Sommer , Nucleic. Acids. Res. / v.20,pp.871-878,
    5. Study of 39 documented relapses of multibacillary leprosy after treatment with rifampin , Groset, J.H.;C.-C. Guelpa-Lauras;P. Bobin;G. Brucker;J.-L. Cartel;M. Constant-Desportes;B. Flageul;M. Frederic;J.-C. Guillaume;J. Millan , Intl. J. Lep. / v.57,pp.607-614,
    6. Detection of rifampin resistance mutation in Mycobacterium tuberculosis and M. leprae. 994 , Imboden, P.;C. Stewart;T. Bodmer;A. Telenti;D.H. Persing(ed.) , Diagnostic molecular microbiology, principles and applications / v.,pp.519-526,
    7. Implications of multidrug resistance of the future of short-course chemotherapy of tuberculosis: a molecular study , Heym, B.;N. Honore;C. Truffot-Pernot;A. Banerjee;C/ Schurra;W.R. Jacobs Jr.;J.D.A. van Embden;J.H. Grosset;S.T. Cole , Lancet / v.344,pp.293-298,
    8. Nucleotide sequence of the first cosmid from the Mycobacterium leprae genome project: Structure and function of the Rif-Str regions , Honore,N.T.;S. Berght;S. Chanteau;F. Doucet-Populaire;K. Eiglmeier;T. Garnier;C. Georges;P. Launois;T. Limpaiboon;S. Newton;K. Niang;P. Del Portillo;G.R. Ramesh;P. Reddi;P.R. Ridel;N. Sittisombut;S. Wu-Hunter;S.T. Cole , Mol. Microbiol. / v.7,pp.207-214,
    9. A rapid method for the detection of potentially viable Mycobacterium leprae in human biopsies: A novel application of PCR , Words, S.A.;S.T. Cole , FRMS Mocrobiol. Lett. / v.65,pp.305-310,
    10. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction , Orita, M.;Y. Suzuki;T. Sekiya;K. Hayashi , Genomics / v.5,pp.874-879,
    11. Molecular basis of rifampin resistance in Mycobacterium leprae , Honore, N.;S.T. Cole , Antimicrob. Agents. Chemother / v.37,pp.414-418,
    12. Detection of polymorphisms of human DNA by gel electrophoresis as single-stand conformation polymorphisms , Orita, M.;H. Iwahana;H. Kanazawa;K. Hayashi;T. Sekiya , Proc. Natl. Acad. Sci. USA / v.86,pp.2766-2770,
    13. The sensitivity of single=stand conformation polymorphism analysis for the detection of single base substitutions , Sheffield, V.C.;J.S. Back;A.E. Kwitek;D.W. Sandstrom;E.M. Stone , Genomics / v.16,pp.325-332,
    14. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance , Jin, D.J.;C.A. Gross , J. Mol. Biol. / v.202,pp.45-58,
    15. Detection of rifampicin-resistance mutation in Mycobacterium tuberculosis , Telenti, A.;P. Imboden;F. Marchesi;D. Lowrie;S. Cole;M.J. Colston;L. Matter;K. Schopfer;T. Bodmer , Lancet / v.341,pp.647-650,
    16. Rifampin-resistant leprosy , Jacobson, R.R.;R.C. Hastings , Lancet / v.2,pp.1304-1305,
    17. The euglena gracillis chloroplast rpoB gene. Novel gene organization and transcription of the RNA prolymerase subunit operon , Yepiz-Plascencia, G.M.;Radebaugh, C.A.;R.B. Hallick , Nucleic. Acids. Res. / v.18,pp.1869-1878,

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