본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The journal of microbiology v.34 no.3, 1996년, pp.248 - 254  

Immunofluorescence Localization of Schizosaccharomjyces pombe $cdc103^{+}$ Gene Product

Kim, Hyong-Bai  
  • 초록

    $cdc103^+$ gene in Schizosaccharomyces pombe which is similar to the CDC3 gene in Saccharomyces cerevisiae was cloned and sequenced. Comparison of the predicted amino acid sequences of $cdc103^+$ and CDC3 revealed that they share significant similarity (43% identity and 56% identity or similarity) to each other. The gene product of CDC3 in S. cerevisiae is known to be a highly ordered ring of filaments that lies just inside the cytoplasmic membrane in the region of the mother-bud neck. In order to characterize the gene product of $cdc103^+$ in Schizosaccharomyces pombe, fusion proteins were used to generate the polyclonal antibodies specific for the gene product (cdc103p). In immunofluorescence experiments, these antibodies decorate the region of the septum formation as a double ring structure late in the cell division cycle.


  • 주제어

    Recombinant plasmid .   pCU103 .   persistence .   transformation .   water microcosm.  

  • 참고문헌 (25)

    1. The Saccharomyces cerevisiae CDC3, CDC10, CDC11, CDC12 genes encode a family of similar proteins , Haarer, B.K.;S.R. Ketcham;S.K. Ford;D.J. Ashcroft;J.R. Pringle , Genetics in press / v.,pp.,
    2. Sequence analysis of the Saccharomyces pombe homologue fo the CDC3 gene in Saccharomyces cerevisiae , Kim, H.B. , Jour. Microbiol. / v.33,pp.350-354,
         
    3. Localization of F-actin through the cell division cycle of Schizsaccharomyces pombe , Marks, J.;J.S. Hyams , Eur. J. Cell Biol. / v.39,pp.27-32,
    4. Growth polarity and cytokinesis in fission in fission yeast : the role of the cytoskeleton , Marks, J.;I.M. Hagan;J.S. Hyams , J. Cell. Sci. (Suppl.) / v.5,pp.229-241,
    5. Phenotypic analysis of temperature sensitive yeast actin mutants , Novick, P.;D. Botstein , Cell / v.40,pp.405-416,
    6. Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle., Localization of the CDC3 gene product and the timing of events at the budding site , Kim, H.B.;B.K. Haarer;J.R. Pringle , J. Cell Biol. / v.112,pp.535-544,
    7. Studies on the organization of 10-nm filament ring in Saccharomyces cerevisiae , Kim, S.C.;J.W. Jung;H.B. Kim , Kor. J. Microbiol. / v.30,pp.333-338,
         
    8. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces , Kilmartin, J.V.;A.E.M. Adams , J. Cell Biol. / v.98,pp.922-933,
    9. Genetic control of the cell division cycle in yeast. Genes controlling bud emergence and cytokinesis , Hartwell, L.H. , Exp. Cell. Res. / v.69,pp.265-276,
    10. Functions of microtubules in the Saccharomyces cerevisiae cell cycle , Jacobs, C.W.;A.E.M. Adams;P.J. Szaniszio;J.R. Pringle , J. Cell Biol. / v.107,pp.1409-1426,
    11. Loss of the filamentous ring in cytokinesis defective mutants of budding yeast , Byers, B.;L. Goetsch , J. Cell Biol. / v.70,pp.35a,
    12. Diverse effects of -tubulin mutations on microtubule formation and function , Huffaker, T.C.;J.H. Thomas;D. Botstein , J. Cell Biol. / v.106,pp.1997-2009,
    13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 , Laemmli, U.K. , Nature / v.227,pp.680-685,
    14. Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC11 gene product and the timing of events at the budding site , Ford, S.K.;J.R. Pringle , Devel. Genet. / v.12,pp.282-292,
    15. The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Saccharomyces pombe , Hagan, I.M.;J.S. Hyama , J. Cell Sci. / v.89,pp.343-357,
    16. Establishment of cell polarity in yeast : Early events in the organization of the budding yeast , Kim, H.B.;S.R. Ketcham;B.K. Haarer;J.R. Pringle , Mol. Cell Biol. / v.,pp.,
    17. Cytology of the yeast life cycle , Byers, B.;J.N. Starthern(ed.);E.W. Jones(ed.);J.R. Broach(ed.) , The molecular biology of the yeast Saccharomyces: Life cycle and inheritance / v.,pp.59-66,
    18. Immunofluorescence localization of the Saccharomyces cerevisiae CDC12 gene products to the vicinity of the 10-nm filaments in the mother-bud neck , Haarer, B.K.;J.R. Pringle , Mol. Cell. Biol. / v.7,pp.3678-3687,
    19. Molecular cloning of the gene in Saccharomyces pombe related to the CDC3 gene in the Saccharomyces cerevisiae , Kim, H.B. , Kor. J. Microbiol. / v.31,pp.197-202,
         
    20. Protein measurement with the filin phenol reagent , Lowry, O.H.;N.J. Rosebrough;A.L. Farr;R.J. Randall , J. Biol. Chem. / v.193,pp.265-275,
    21. The Saccharomyces cerevisiae cell cycle , Pringle, J.R.;L.H. Hartweel;J.N. Strathern(ed.);E.W. Jones(ed.);J.R. Broach(ed.);J.N. Strathern;E,W. Jones;J.R. Broach , The molecular biology of the yeast Saccharomyces : Life cycle and inheritance / v.,pp.97-142,
    22. Relation of actin and tubulin distribution to bud growth in wild type and morphogenetic mutant Saccharomyces cerevisiae , Adams, A.E.M.;J.R. Pringle , J. Cell Biol. / v.98,pp.935-945,
    23. Phylogenetic relationships among species of Saccharomyces, Schizo-saccharomyces, Debaromyces, and Schwanniomyces determined from partial ribosomal RNA sequences , Kurtzman, C.P.;C.J. Robnett , Yeast / v.7,pp.61-72,
    24. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedures and some applications , Towbin, H.;T. Staehelin;J. Gordon , Proc. Natl. Acad. Sci. USA / v.76,pp.4350-4354,
    25. Fluorescence microscopy methods for yeast , Pringle, J.R.;R.A. Preston;A.E.M. Adams;T. Sterns;D.G. Drutin;B.K. Haarer;E.W. Jones , Meth. Cell Biol. / v.31,pp.357-435,

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기