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The journal of microbiology v.34 no.3, 1996년, pp.284 - 288  

Fungal-Sporulation Suppressing Substances Produced by Pseudomonas aeruginosa KMCS-1

Min, Bu-Yong    (Department of Microbiology, Kangwon National University   ); Shim, Jae-Young    (Department of Microbiology, Kangwon National University   ); Kim, Kun-Woo    (Department of Resources Plant, Andong National University   ); Lee, Jong-Kyu    (Department of Forest Resources Protection Kangwon National University   ); Choi, Hyung-Tae    (Department of Microbiology, Kangwon National University   ); Yoon, Kwon-Sang    (Department of Microbiology, Kangwon National University  );
  • 초록

    Among the bacteria isolated from compost piles of cattle excretion in a pasture located at the suburbs of Chunchon city, Pseudomonas aeruginosa KMCS-1 was selected for the test of antifungal substances produced. Six fractions were separated by silica gel column chromatography, and then the antifungal activity of each fraction was assayed against Escherichia coli, Bacillus subtilis, Candida albicans, Rhizopus sp., Aspergillus nidulans, Coprinus cinereus, and Pyricularia oryzae by paper disc method. Two fractions showed significant suppressive activities against A. nidulans, C. cinereus, and P. oryzae however, their mycelial growth was not affected by neither of these fractions. Inhibitory activities of these fractions to sporulation was assayed at the concentration of 50. 25, 12. 5, and 6.25 $\mu$ g/ml and the average inhibition rates against sporulation of A. nidulans, C. cinereus, and P. oryzae were 94.0, 98.3, and 77.9%, respectively. Further purification and analysis of active substances are now being conducted.


  • 주제어

    Pseudomonas aeruginosa .   antifungal substance .   asexual sproulation .   silica gel chroma tography .   bioassay.  

  • 참고문헌 (27)

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    25. Novel antifungal tetracyclic compounds from Bauhinia rufescens , Maillard, M.P.;I. Recio;M. Saadou;H.S. Evans;K. Hostettmann , LAM. HELVETICA CHIMICA ACTA / v.74,pp.791-799,
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    27. Evaluation activity of fluorescent Pseudomonads against some rice fungal diseases in vitro and greenhouse , Lee, Y.H.;G.Y. Shim;E.J. Lee;T.W. Mew , Kor. J. Plant Pathol. / v.6,pp.73-80,

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