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The journal of microbiology v.34 no.4, 1996년, pp.379 - 383  

Production of a Monoclonal Antibody and Ultrastructure of the Sporozoite of Cryptosporidium parvum

Choi, Young-Sook    (Department of Clinical Pathology   ); Lee, Sung-Tae    (Kon-Kuk university   ); Cho, Myung-Hwan    (Department of Biology, College of Sciences  );
  • 초록

    Cryptosporidium parvum causes a life-threatening diarrhea in acquired immunodeficiency syndrome (AIDS) patients. THe sporozoite stage of C. parvum has been known to be a target in treating cryptosporidiosis in AIDS patients as it is an extracellular stage. A sporozoite was ultrastructurally observed. It has a creascent shape with a rounded posterior end and a tapering body. The compact nucleus was located at the posterior end. A monoclonal antibody was produced, which recognized a 43 kDa of sporozoite antigens in a western blot analysis and showed the surface labeling in immunofluorescence.


  • 주제어

    Cryptosporidium .   AIDS .   sporozoite .   ultrastructure .   monoclonal antibody.  

  • 참고문헌 (16)

    1. comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection , Arrowood, M.J.;C.R. Sterling , J. Clin. Microbiol. / v.27,pp.1490-1495,
    2. Infection and diarrhea caused by Cryptosporidium sp. among Guatemalan infants , Cruz, J.R.;F. Cano;P. Caceres;F. Chew;G. Pareja , J. Cln. Microbiiol. / v.26,pp.88-91,
    3. Detection of Cryptosporidium sp. infections using a direct immunofluorescent assay , Sterling, C.R.;M.J. Arrowood , Ped. Inf. Dis. / v.5,pp.139-142,
    4. Quantification of specific antibody response to Cryptosporidium antigens by laser densitometry , Ungar, B.L.P.;T.E. Nash , Infect. Immun. / v.53,pp.124-128,
    5. Cryptosporidium parvum, a coccidium found in the small intestine of the common mouse , Tyzzer, E.E. , Arch. Protistenkd. / v.26,pp.394-412,
    6. Passive transfer of immunity against Cryptosporidium infection in neonatal mice using monoclonal anitbodies , Cho, M.H. , K. J. Parisotiology. / v.31,pp.223-230,
    7. Detection of Cryptosporidium oocysts from out-patients of the Severance Hospital, Korea , Cho, M.H.;A.K. Kim;K.I. Im , K. J. Parasitology / v.31,pp.193-199,
         
    8. Cryptosporidiosis: a review , Anderson, B.C. , J. Am. Vet. Med. Assoc. / v.180,pp.1455-1457,
    9. Gastroenteritis due to Cryptosporidium: a prospective survey in a children's hospital , Hart, C.A.;D. Baxby;N. Blundell , J. Infect. / v.9,pp.264-270,
    10. Effects of immune colostrum and orally administered antisporozoite monoclonal antibodies on the outcome of Cryptosporidium parvum infections in neonatal mice , Arrowood, M.J.;J.R. Mead;J.L. Mahrt;C.R. Sterling , Infec. Immun. / v.57,pp.2283-2288,
    11. Cryptosporidium and cryptosporidiosis , Current, W.L.;Gottlib, M.S.(ed.);Groopman, J.E.(ed.) , Acquired Immune Deficiency Syndrome, UCLA Symp. Molecular Cellular Bio., New Series / v.16,pp.355-378,
    12. Cryptosporidium as a causative agent of traveler's diarrhea , Sterling, C.R.;K. Seegar;N.A. Sinclair , J. Infect. Dis. / v.153,pp.380-381,
    13. Fluorescence detection of Cryptosporidium oocysts in human fecal specimens by using monoclonal antibodies , Garcia, L.S.;T.C. Brewer;D.A. Bruckner , J. Clin. Microbiol. / v.25,pp.119-121,
    14. Cryptosporidium in acquired immunodeficiency syndrome , Cohen, J.D.;L. Ruhlig;S.A. Jayich;M.F. Tong;J. Lachago;W.J. Snape , Dig. Dis. Sci. / v.29,pp.773-777,
    15. Antigens of Cryptosporidium sporozoites recognized by immune sera of infected animals and humans , Mead, J.R.;M.J. Arrowood;C.R. Sterling , J. Parasit. / v.19,pp.135-143,
    16. Recognition of whole Cryptosporidium oocysts in feces by negative staining and electron microscopy , Baxby, S.;B. Getty;N. Blundell;S. Ratchliffe , J. Clin. Microbiol. / v.19,pp.566-567,

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