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Isolation and Characterization of a Pollen-specific cDNA Clone from Easter Lily

Kim, Seong-Ryong   (Department of Life Science, Sogang UniversityUU0000674  ); An, Gyu-Heung   (Department of Life Science, Pohang University of Science and TechnologyUU0001353  );
  • 초록

    A pollen-specific cDNA clone, LMP50, was isolated from the mature pollen cDNA library of the Easter lily. The LMP50 transcript was highly abundant in mture pollen grains but not detectable in other organs. The LMP50 cDNA clone contains 1383 nucleotides and two open reading frames. The first codes for a peptide of 15 amino acid residues. The role of this peptide is nuclear. The second encodes a protein containing 329 amino acid residues. This protein exhibited a significant homology to human tartrate-resistant acid phosphatase and porcine uteroferrin. Both of these enzymes have been suggested to play a role in iron transport. Therefore, LMP50 may act as an iron carrier protein in mature pollen grains.


  • 주제어

    pollen .   Easter lily .   acid phosphatase .   uteroferrin .   iron transport.  

 저자의 다른 논문

  • Kim, Seong-Ryong (18)

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    2. 1998 "Analysis of 176 Expressed Sequence Tags Generated from cDNA Clones of Hot Pepper by Single-pass Sequencing" Journal of plant biology = 식물학회지 41 (2): 116~124    
    3. 1999 "Expression and Characterization of Human T-Cell Leukemia Virus Type-I Env and Gag Proteins" Journal of microbiology and biotechnology 9 (3): 311~317    
    4. 1999 "Expression of Human Thrombopoietin in Insect Cells by Polyhedrin-gp64 Dual Promoter-Based Baculovirus Vector System" Journal of microbiology and biotechnology 9 (3): 318~322    
    5. 1999 "Molecular Characterization of an Apple cDNA Encoding Cinnamyl Alcohol Dehydrogenase" Journal of microbiology and biotechnology 9 (4): 475~481    
    6. 2000 "Expressed Sequence Tags of Young Floral Buds and Characterization of a Bud-Preferential Lectin-Like cDNA from Pharbitis nil" Journal of plant biology = 식물학회지 43 (3): 171~178    
    7. 2003 "Molecular Characterization of a Fruit-Preferential Thaumatin-Like Gene from Apple (Malus domestica cv. Fuji)" Journal of plant biology = 식물학회지 46 (1): 52~58    
    8. 2003 "Identification of a Flower-Specific cDNA, RsPCP1, Encoding Putative Pollen Coat Protein from Radish" Journal of plant biology = 식물학회지 46 (2): 130~133    
    9. 2004 "Characterization of a Cold-Responsive Gene, OsPTR1, Isolated from the Screening of $\beta$-Glucuronidase (GUS) Gene-Trapped Rice" Journal of plant biology = 식물학회지 47 (2): 133~141    
    10. 2005 "Characterization of a Drought-Responsive Gene, OsTPS1, Identified by the T-DNA Gene-Trap System in Rice" Journal of plant biology = 식물학회지 48 (4): 371~379    
  • An, Gyu-Heung (1)

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