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Journal of plant biology = 식물학회지 v.39 no.1, 1996년, pp.9 - 13   피인용횟수: 1

백합실물에서 하나으 Calmodulin cDNA 클론 연구
Characterization of an Easter Lily Calmodulin cDNA Clone

Kim, Seong-Ryong  
  • 초록

    A clone, LCM1, which encodes calmodulin (CaM) was isolated and characterized from monocot lily (Lilium longiflorum Thunb.) plants. The clone is 681 bps and contains the 447 bp coding region, 8 bp leader sequence, 210 bp 3'-untraslated region, and a poly(A) tail. The coding region of 149 amino acids encodes a protein of predicted Mr 17 kD. Comparison of the LCM1 amino acid sequence with other CaMs revealed that the protein is highly conserved among various living organisms. The expression level of calmodulin gene in lily was studied by RNA blot analysis. The LCM1 mRNA was present in all tissues tested. However, a higher level of calmodulin was observed in anther and floral bud. The level of calmodulin mRNA in anther was about 10 times higher than that in anther was about 10 times higher than that in vegetative tissues. The anther preferential expression of CaM in lily is currently investigated in dicot plants.


  • 주제어

    꽃 .   백합 .   약-우세적 .   calmodulin .   cDNA .   flower .   Easterlily .   anther-preferential.  

  • 이 논문을 인용한 문헌 (1)

    1. 2001. "" Journal of plant biology = 식물학회지, 44(1): 33~40     

 저자의 다른 논문

  • Kim, Seong-Ryong (18)

    1. 1996 "Isolation and Characterization of a Pollen-specific cDNA Clone from Easter Lily" Journal of plant biology = 식물학회지 39 (3): 197~202    
    2. 1998 "Analysis of 176 Expressed Sequence Tags Generated from cDNA Clones of Hot Pepper by Single-pass Sequencing" Journal of plant biology = 식물학회지 41 (2): 116~124    
    3. 1999 "Expression and Characterization of Human T-Cell Leukemia Virus Type-I Env and Gag Proteins" Journal of microbiology and biotechnology 9 (3): 311~317    
    4. 1999 "Expression of Human Thrombopoietin in Insect Cells by Polyhedrin-gp64 Dual Promoter-Based Baculovirus Vector System" Journal of microbiology and biotechnology 9 (3): 318~322    
    5. 1999 "Molecular Characterization of an Apple cDNA Encoding Cinnamyl Alcohol Dehydrogenase" Journal of microbiology and biotechnology 9 (4): 475~481    
    6. 2000 "Expressed Sequence Tags of Young Floral Buds and Characterization of a Bud-Preferential Lectin-Like cDNA from Pharbitis nil" Journal of plant biology = 식물학회지 43 (3): 171~178    
    7. 2003 "Molecular Characterization of a Fruit-Preferential Thaumatin-Like Gene from Apple (Malus domestica cv. Fuji)" Journal of plant biology = 식물학회지 46 (1): 52~58    
    8. 2003 "Identification of a Flower-Specific cDNA, RsPCP1, Encoding Putative Pollen Coat Protein from Radish" Journal of plant biology = 식물학회지 46 (2): 130~133    
    9. 2004 "Characterization of a Cold-Responsive Gene, OsPTR1, Isolated from the Screening of $\beta$-Glucuronidase (GUS) Gene-Trapped Rice" Journal of plant biology = 식물학회지 47 (2): 133~141    
    10. 2005 "Characterization of a Drought-Responsive Gene, OsTPS1, Identified by the T-DNA Gene-Trap System in Rice" Journal of plant biology = 식물학회지 48 (4): 371~379    

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