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Journal of microbiology and biotechnology v.6 no.4, 1996년, pp.295 - 298   피인용횟수: 1

The Effect of Glutamine on Production of Tissue-type Plasminogen Activator from Recombinant Human Melanoma Cells in Glutamine-limited Fed-batch Cultivation

Kim, Hyun-Goo   (Division of Food and Biotechnology, Kangwon National UniversityUU0000037  ); Kim, Tae-Ho   (Division of Food and Biotechnology, Kangwon National UniversityUU0000037  ); Kim, Dae-Seok   (Division of Food and Biotechnology, Kangwon National UniversityUU0000037  ); Park, Kyung-Yu   (Department of Medicine, Hanllym University  ); Park, jin-seo   (Department of Genetic Engineering, Hanllym University  ); Ahn, Chol   (Division of Food and Biotechnology, Kangwon National UniversityUU0000037  ); Lee, Jin-Ha   (Division of Food and Biotechnology, Kangwon National UniversityUU0000037  ); Lee, Hyeon-Yong   (Division of Food and Biotechnology, Kangwon National UniversityUU0000037  );
  • 초록

    Under glutamine-limited condition, $2\times10^6$ (viable cells/ml) of maximum cell density and 13.5 ( $\mu g$ /ml) of tissue-type Plasminogen Activators (tPA) production were maintained by spike feeding fresh medium in fed-batch cultivation of human recombinant melanoma cells. It showed that tPA production was much seriously affected than cell growth according to initial glutamine concentrations. Above 3.4 (mmol/I) of glutamine concentration both cell growth and tPA production were not much affected by increasing initial glutamine concentration. Glutamine depleted situation was occurred at latter periods of batch and fed-batch cultivations below 5.4 (mmole/I) of initial glutamine concentration. It also showed that maximum glutamine consumption and ammonia evolution rates were closely related to initial glutamine concentrations. Maximum specific tPA production rate was estimated as $8.1\times19^{-6}$ ( $\mu g$ /cells/h) at 3.4(mmol/I) of glutamine concentration, which is higher than that from other batch and fed-batch processes.


  • 주제어

    tPA .   human melanoma cells .   glutamine-limited .   fed-batch cultivation.  

  • 이 논문을 인용한 문헌 (1)

    1. 2001. "" Biotechnology and bioprocess engineering, 6(1): 11~17     

 저자의 다른 논문

  • Kim, Hyun-Goo (1)

    1. 1996 "저혈청 배지에서 인간 전골수세포(HL-60)를 이용한 tPA 생산과 세포사멸기작에 관한 연구" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 24 (1): 126~131    
  • Kim, Tae-Ho (1)

  • Ahn, Cheol (8)

  • Lee, Jin-Ha (43)

  • 이현용 (108)

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