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Journal of microbiology and biotechnology v.6 no.5, 1996년, pp.315 - 320   피인용횟수: 1

Site-specific Disruption of Glyoxylate Bypass and Its Effect in Lysine-producing Corynebacterium lactofermentum Strain

Kim, Youn-Hee  
  • 초록

    The role of glyoxylate bypass in a lysine-producing Corynebacterium lactofermentum strain was analyzed. Unlike the wild type, the strain expressed enzymes of glyoxylate bypass during growth in the fermentation broth containing glucose as the carbon source. To evaluate the importance of glyoxylate bypass in the strain, we disrupted chromosomal aceA by using a cloned fragment of the gene. Site-specific disruption of aceA which codes for the isocitrate lyase, the first enzyme of the bypass, was confirmed by Southern blot analysis. The aceA mutant strain completely lost isocitrate lyase activity and ability to grow in a minimal medium containing acetate as the sole carbon source. The mutant strain was similar to its parental strain in growth characteristics and produced comparable amounts of lysine in shake flasks containing glucose as the carbon source. The amount of oxaloacetate accumulated in the fermentation medium was similar for both strains, suggesting that expression of glyoxylate bypass does not necessarily lead to the increase in intracellular oxaloacetate. These data clearly demonstrate that glyoxylate bypass does not function as one of the routes of carbon supply for lysine production in the strain. It appears that the leakiness of the glyoxylate bypass in the strain might be the result of a secondary mutation which arose during previous strain development by random mutagenesis.


  • 주제어

    Corynebacterium lactofermentum .   glyoxylate bypass .   aceA .   lysine.  

  • 이 논문을 인용한 문헌 (1)

    1. Kim, Tae-Hyun ; Namgoong, Suk ; Kwak, Joon-Hyeok ; Lee, Se-Yong ; Lee, Heung-Shick 2000. "Effects of tktA, $aroF^{FBR}$and aroL Expression in the Tryptophan-Producing Escherichia coli" Journal of microbiology and biotechnology, 10(6): 789~796     

 저자의 다른 논문

  • Lee, Heung-Shick (23)

    1. 1994 "Metabolic control of aspartate-derived amono acid production in Corynebacterium alutamicum" 微生物과 産業 = The microorganisms and industry 20 (2): 2~8    
    2. 1996 "Molecular Cloning of the Arginine Biosynthetic Genes from Corynebacterium glutamicum" The journal of microbiology 34 (4): 355~362    
    3. 1997 "Gene Amplification of aceA and aceB in Lysine-producing Corynebacterium glutamicum ssp. lactofermentum ATCC21799" Journal of microbiology and biotechnology 7 (5): 287~292    
    4. 1997 "$Ca^{2+}$ is Required to Make Functional Malate Synthase in Corynebacterium glutamicum" Journal of microbiology and biotechnology 7 (6): 435~437    
    5. 1998 "탈질화성 페놀 분해균 Pseudomonas sp. HL100의 분리 및 특성" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 26 (4): 303~308    
    6. 2000 "In Vitro Proliferation Model of Helicobacter pylori Required for Large-Scale Cultivation" Journal of microbiology and biotechnology 10 (3): 367~374    
    7. 2000 "Effects of tktA, $aroF^{FBR}$and aroL Expression in the Tryptophan-Producing Escherichia coli" Journal of microbiology and biotechnology 10 (6): 789~796    
    8. 2001 "Improvement of Decarboxylating Agar Medium for Screening Biogenic Amine-Producting Bacteria in Kimchi" Journal of microbiology and biotechnology 11 (3): 491~496    
    9. 2001 "Analysis of metabolic pathways and amino acid production in Coynebacterium glutamicum" 微生物과 産業 = The microorganisms and industry 27 (2): 30~39    
    10. 2003 "Isolation and Analysis of the argG Gene Encoding Argininosuccinate Synthetase from Corynebacterium glutamicum" Journal of microbiology and biotechnology 13 (6): 949~954    

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