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Journal of microbiology and biotechnology v.6 no.6, 1996년, pp.391 - 396   피인용횟수: 1

$Na^{+}$-dependent NADH:quinone Oxidoreductase in the Respiratory Chain of the Marine Bacterium Marinomonas vaga

Kim, Young-Jae   (Department of Microbiology, Changwon National UniversityUU0001233  ); Park, Yong-Ha   (Korean College for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and TechnologyCC0186926  );
  • 초록

    The Gram-negative marine bacterium Marinomonas vaga, which requires 0.5 M NaCl concentration for optimal growth, is slightly halophilic. The growth of M vaga was highly resistant to the proton conductor, carbonyl cyanide m-chlorophenylhydrazone (CCCP) under alkaline pH conditions (pH 8.5) but very sensitive to CCCP under acidic pH conditions (pH 6.5). These results suggest that the respiratory chain-linked NADH oxidase system of M. vaga may lead to generation of a $Na^{+}$ electrochemical gradient. In order to examine the existence of $Na^{+}$ -stimulated NADH oxidase in M. vaga, membrane fractions were prepared by the osmotic lysis method. The membrane-bound NADH oxidase oxidized both NADH and deamino-NADH as substrates and required $Na^{+}$ for maximum activity. The maximum activity of NADH oxidase was obtained at about pH 8.5 in the presence of 0.2 M NaCl. The site of $Na^{+}$ -dependent activation in the NADH oxidase system was at the NADH:quinone oxidoreductase segment. The NADH oxidase and NADH:quinone oxidoreductase were very sensitive to the respiratory chain inhibitor, 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO) in the presence of 0.2 M NaCl but highly resistant to another respiratory inhibitor, rotenone. Based on these findings, we conclude that M. vaga possesses the $Na^{+}$ -dependent NADH:quinone oxidoreductase that may function as an electrogenic $Na^{+}$ pump.


  • 주제어

    halophilic marine bacteria .   Marinomonas vaga .   respiratory chain .   $Na^{+}$-dependent NADH: quinone oxidoreductase .   $Na^{+}$ pump.  

  • 이 논문을 인용한 문헌 (1)

    1. 2003. "" Journal of microbiology and biotechnology, 13(2): 225~229     

 저자의 다른 논문

  • Kim, Young-Jae (14)

    1. 1998 "Taxonomic Studies of the Beta Hemolysis-causing Pathogen Bacillus cereus Isolated from Sea Water" Journal of microbiology and biotechnology 8 (1): 67~73    
    2. 1999 "해양 호염성 세균 Vibrio alginolyticus가 생산하는 Extracellular Amylase의 특성" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 27 (3): 203~207    
    3. 2000 "The Succinate : Quinone Oxidoreductase of Marine Bacterium Vibiro alginolyticus is a $H^+$" Journal of microbiology and biotechnology 10 (1): 48~50    
    4. 2000 "Influence of Temperature, Oxygen, m-Chlorophenylhydrazone Cerulenin, and Quinacrine on the Production of Extracellular Proteases in Bacillus cereus" Journal of microbiology and biotechnology 10 (1): 103~106    
    5. 2001 "Thin Layer Chromatogram by an Extracellular ${\beta}$-Amylase of Bacillus sp. KYJ 963 and its Amino Acid Composition" Journal of life science 11 (2): 92~93    
    6. 2003 "The Membrane-Bound NADH:Ubiquinone Oxidoreductase in the Aerobic Respiratory Chain of Marine Bacterium Pseudomonas nautica" Journal of microbiology and biotechnology 13 (2): 225~229    
    7. 2004 "자생식물 추출물의 세포 분산 활성" 생약학회지 35 (1): 62~79    
    8. 2004 "Enzymatic Properties of the Membrane-bound NADH Oxidase System in the Aerobic Respiratory Chain of Bacillus cereus" Journal of biochemistry and molecular biology = 한국생화학회지 37 (6): 753~756    
    9. 2004 "The Influence of NaCl and Carbonylcyanide-m-Chlorophenylhydrazone on the Production of Extracellular Proteases in a Marine Vibrio Strain" The journal of microbiology 42 (2): 156~159    
    10. 2005 "Enzymatic and Energetic Properties of an Aerobic Respiratory Chain­Linked NADH Oxidase System in Marine Bacterium Vibrio natriegens" Journal of microbiology and biotechnology 15 (5): 1080~1086    

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