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Renal mRNA Expression of Renin, $AT_1$ Receptor, TGF-${\beta}1$ and Fibronectin in Obstructive Nephropathy

Yang, Eun-Kyoung   (Department of Physiology, School of Medicine, Kyungpook National UniversityUU0000096  ); Kim, In-Kyeom   (Department of Pharmacology, School of Medicine, Kyungpook National UniversityUU0000096  );
  • 초록

    The present study was designed to quantify the alterations of renal renin, angiotensin type I receptor ( $AT_1$ ), $TGF-{\beta}1$ , and fibronectin gene expression in rats with unilateral ureteral obstruction (UUO). We also investigated the change of $AT_1$ density during UUO. Reverse transcription-polymerase chain reaction (RT-PCR) technique and receptor binding assay were used to detect mRNA expression and receptor density, respectively. At one day after UUO, renin mRNA level of the obstructed kidneys was decreased transiently and then subsequently increased to the level of sham kidneys. In the contralateral kidneys of the same rats, on the contrary, renin mRNA level was gradually decreased. Then, at 9 days after UUO, it was significantly lower than that of sham kidneys. The expressions of both $AT_1$ subtypes, called $AT_{1A}$ and $AT_{1B}$ , mRNAs did not change at any time. UUO led to a significant decrease in $AT_1$ density in the obstructed kidneys compared with the sham kidneys at 1 and 3 days $(66\;{\pm}\;11.6%\;(p (p $AT_1$ density was gradually increased and at 9 days it showed a marked elevation in the obstructed kidneys compared to the sham kidneys. In contrast, in the contralateral kidneys $AT_1$ density was significantly reduced from 3 to 9 days after UUO. The $TGF-{\beta}$ 1 mRNA level of the obstructed kidneys was unexpectedly decreased at 6 days after UUO. Then, at 9 days it was followed by a significant increase in the obstructed kidneys, whereas it showed an obvious decrease in the contralateral kidneys. In addition, fibronectin mRNA level was also significantly increased in the obstructed kidneys after UUO compared to the sham or the contralateral kidneys of the same rats. These results suggest a differential regulation of renal renin, $AT_1$ receptor, $TGF-{\beta}$ 1 and fibronectin mRNA levels at different stages of UUO.


  • 주제어

    Renin .   $AT_1$ receptor .   TGF- ${\beta}1$ .   Fibronectin .   RT-PCR .   Unilateral ureteral obstruction.  

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  • 양은경 (10)

    1. 1993 "Acute and Chronic Effects of Ethanol on the Cardiovascular and Hormonal Responses to Hemorrhage in Conscious Normotensive and Spontaneously Hypertensive Rats" 대한생리학회지 = Korean journal of physiology 27 (1): 67~77    
    2. 1993 "Effects of Centrally Administered Angiotensin ll Receptor Antagonists on the Cardiovascular and Hormonal Responses to Hemorrhage in Conscious SHR" 대한생리학회지 = Korean journal of physiology 27 (2): 217~225    
    3. 1996 "흡입마취제 투여시 내독소혈증흰쥐 대동맥 수축반응에 미치는 Hydroxocobalamin의 효과" The Korean journal of pharmacology : official journal of the Society of Pharmacology, Republic of Korea 32 (3): 381~388    
    4. 1997 "Gene Expression of Intrarenal Renin-angiotensin System in Streptozotocin-induced Diabetic Rats" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 1 (1): 45~53    
    5. 1997 "Effect of Hemorrhage on mRNA Expressions of Renin, Angiotensinogen and $AT_1$ Receptors in Rat Central and Peripheral Tissues" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 1 (2): 151~159    
    6. 1999 "Spectral Analysis of Heart Rate Variability during Passive Standing after Ethanol Ingestion" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 3 (6): 605~613    
    7. 2000 "Effect of Brain Angiotensin II Receptor Antagonists and Antisense Oligonucleotide on Drinking and Renal Renin in Rats" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 4 (2): 137~142    
    8. 2001 "Role of Angiotensin II and Nitric Oxide in the Rat Paraventricular Nucleus" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 5 (1): 41~46    
    9. 2001 "Pulse Exposure to Ethanol Augments Vascular Contractility Through Stress Response" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 5 (1): 47~53    
    10. 2006 "Involvement of ERK1/2 and JNK Pathways in 17${\beta}-estradiol$ Induced Kir6.2 and SK2 Upregulation in Rat Osteoblast-like Cells" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 10 (4): 199~205    
  • Kim, In-Kyeom (10)

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