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Lipocortin 1 Mediates the Suppressive Effects of Dexamethasone on ConA-induced Proliferative Response and Nitric Oxide Production in Rat Splenic Leukocytes

Jang, Yeon-Jin   (Departments of Physiology, University of Ulsan College of MedicineUU0001014  ); Park, Hyoung-Sup   (Departments of Pharmacology, University of Ulsan College of MedicineUU0001014  ); Kang, Soon-A   (Departments of Pharmacology, University of Ulsan College of MedicineUU0001014  ); Yang, Sus-Jung   (Departments of Physiology, University of Ulsan College of MedicineUU0001014  ); Na, Doe-Sun   (Departments of Biochemistry, University of Ulsan College of MedicineUU0001014  );
  • 초록

    Lipocortin 1 has been proposed as a putative mediator of anti-inflammatory actions of glucocorticoids. We investigated the role of lipocortin 1 in the effect of dexamethasone using rat splenic leukocytes. Concanavalin A(ConA; 1 ${\mu}g/ml$ ) increased the leukocyte proliferation and nitric oxide(NO) generation, which were measured as $[^3H]-thymidine$ uptake by the cells and nitrite accumulation in the culture media, respectively. Dexamethasone suppressed ConA-induced cell proliferation, in a concentration-dependent manner with $EC_{50}$ around 50nM. The addition of anti-lipocortin l(Anti-LCl) reversed dexamethasone effects: 0.24, 1.2, 6 ${\mu}g/ml$ of Anti-LC1 reversed dexamethasone(50 nM)-induced suppression of thymidine uptake by $9{\pm}3%$ , $16{\pm}3%$ , $36{\pm}5%$ , respectively; 0.24, 1.2, and 6 ${\mu}g/ml$ of Anti-LCI reversed dexa-methasone-induced decrease of nitrite concentration by $49{\pm}16%$ , $61{\pm}20%$ , $77{\pm}19%$ , respectively. The present data indicate that lipocortin 1 mediates, at least in part, glucocorticoids-induced suppression of leukocyte proliferation and blockade of NO generation.


  • 주제어

    Lipocortin 1 .   Dexamethasone .   Thymidine uptake .   Nitric oxide production.  

 저자의 다른 논문

  • 장연진 (5)

    1. 1993 "당뇨성 심근질환에서의 근장그물 기능이상과 그 작용기전" The Korean journal of pharmacology : official journal of the Society of Pharmacology, Republic of Korea 29 (2): 195~202    
    2. 1995 "당뇨병성 신증의 발생에 있어서 산화성 스트레스의 역할" The Korean journal of pharmacology : official journal of the Society of Pharmacology, Republic of Korea 31 (1): 95~102    
    3. 1998 "Transient Increase of Lipocortin 1 in Nuclei of the Hippocampal Pyramidal Neurons in Rats Induced by Immobilization Stress" Journal of biochemistry and molecular biology = 한국생화학회지 31 (2): 117~122    
    4. 1999 "Role of $Ca^{2+}$ in the Stimulation of Glucose Transport by Insulin in Adipocytes" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 3 (3): 357~364    
  • Park, Hyoung-Sup (12)

  • Na, Doe-Sun (20)

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