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Nitric Oxide Prevents the Bovine Cerebral Endothelial Cell Death Induced by Serum-Deprivation

Kim, Chul-Hoon   (Yonsei Brain Research Institute and Department of Pharmacology, Yonsei University College of MedicineUU0000936  ); Ahn, Young-Soo   (Yonsei Brain Research Institute and Department of Pharmacology, Yonsei University College of MedicineUU0000936  );
  • 초록

    Endothelial cells play a central role in the inflammatory processes, and activation of nuclear factor kappa B ( $NF-_{\kappa}B$ ) is a key component in that inflammatory processes. Previously, we reported that tumor necrosis factor alpha( $TNF{\alpha}$ ) had protective effect of cell death induced by serum deprivation and this protection was related to $NF-_{\kappa}B$ activation. Inducible nitric oxide synthase (iNOS) is a member of the molecules which transcription is regulated mainly by $NF-_{\kappa}B$ . And the role of nitric oxide (NO) generated by iNOS on cell viability is still controversial. To elucidate the mechanism of $TNF{\alpha}$ and $NF-_{\kappa}B$ activation on cell death protection, we investigate the effect of NO on the cell death induced by serum- deprivation in bovine cerebral endothelial cells in this study. Addition of $TNF{\alpha}$ , which are inducer of iNOS, prevented serum-deprivation induced cell death. Increased expression of iNOS was confirmed indirectly by nitrite measurement. When selective iNOS inhibitors were treated, the protective effect of $TNF{\alpha}$ on cell death was partially blocked, suggesting that iNOS expression was involved in controlling cell death. Exogenously added NO substrate (L-arginine) and NO donors (sodium nitroprusside and S-nitroso-N-acetylpenicillamine) also inhibited the cell death induced by serum deprivation. These results suggest that NO has protective effect on bovine cerebral endothelial cell death induced by serum-deprivation and that iNOS is one of the possible target molecules by which $NF-_{\kappa}B$ exerts its cytoprotective effect.


  • 주제어

    Endothelial cell death .   iNOS .   NO .   $NF-_{\kappa}B$ .   $TNF_{\alpha}$.  

 저자의 다른 논문

  • Kim, Chul-Hoon (3)

    1. 1996 "모래쥐에서 뇌의 허혈/재관류에 의한 산화성 스트레스 형성과 HSP70의 발현" The Korean journal of pharmacology : official journal of the Society of Pharmacology, Republic of Korea 32 (3): 335~345    
    2. 1999 "Role of Protein Kinases on NE-$_{\kappa}B$ Activation and Cell Death in Bovine Cerebral Endothelial Cells" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 3 (1): 11~18    
    3. 2003 "Inhibition of $NF-{\kappa}B$ Activation Increases Oxygen-Glucose Deprivation-Induced Cerebral Endothelial Cell Death" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 7 (2): 65~71    
  • Ahn, Young-Soo (16)

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