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Endothelin Increases Intracellular Free Calcium in Isolated Rat Nephron

Cha, Seok-Ho   (Department of Pharmacology and Toxicology, Kyorin University School of MedicineUU0016171  ); Cho, Young-Jin   (Department of Pharmacology, Catholic University Medical CollegeUU0013933  ); Lee, Kweon-Haeng   (Department of Pharmacology, Catholic University Medical CollegeUU0013933  ); Endou, Hitoshi   (Department of Pharmacology and Toxicology, Kyorin University School of MedicineUU0016171  );
  • 초록

    In the freshly isolated rat nephron, the effect of endothelin-1, -2 and -3 (ET-1, -2 and -3) on cytosolic free calcium concentration ( $[Ca^{2+}]_i$ ) was determined using the fluorescent indicator Fura-2/AM. $[Ca^{2+}]_i$ increase was investigated in 9 parts of the single nephron including glomerulus (Glm), $S_1,\;S_2,\;S_3$ , cortical and medullary thick ascending limb and cortical (CCT) and outer medullary collecting tubule (OMCT). Endothelins increased $[Ca^{2+}]_i$ in Glm (ET-1; $127{\pm}17%$ , ET-2; $93{\pm}5%$ , ET-3; $169{\pm}17%$ ), CCT (ET-1; $30{\pm}6%$ , ET-2; $38{\pm}19%$ , ET-3; $158{\pm}18%$ ) and OMCT (ET-1; $197{\pm}11%$ , ET-2; $195{\pm}11%$ , ET-3; $215{\pm}37%$ ) at 10-7 M. In OMCT, ET-1 and ET-2 increased $[Ca^{2+}]_i$ in a dose-dependent manner ( $10^{-10}{\sim}10^{-6}$ M). To the contrary, ET-3-induced $[Ca^{2+}]_i$ rise was begun from $10^{-12}$ M. BQ-123Na, an antagonist of ETA receptor, at $10^{-4}$ M inhibited about 30% of $[Ca^{2+}]_i$ rise induced by ET-1 and -3. Binding experiments using $[^{125}I]ET-3$ showed the existence of $ET_B$ receptor in OMCT. This binding was replaced by ET-1, ET-2 or ET-3 by the almost same degree but not by angiotensin II or vasopressin.


  • 주제어

    Endothelin .   Intracellular free calcium .   Outer medullary collecting tubule.  

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    3. 1998 "Hydroxyl Radical-Mediated Commitment of HL-60 Cells to Differentiation: Modulation of Differentiation Process by Phosphodiesterase Inhibitors" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2 (3): 369~376    
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  • Lee, Kweon-Haeng (8)

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