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쥐 척수후각세포의 유해자극 반응에 대한 칼슘이온통로 차단제의 억제작용
Calcium Channel Blockers Suppress the Responses of Rat Dorsal Horn Cell to Nociceptive Input

강석한   (한양대학교 의과대학 생리학교실 및 의과학연구소UU0001519  ); 김기순   (한양대학교 의과대학 생리학교실 및 의과학연구소UU0001519  ); 신홍기   (Department of Physiology, School of Medicine, Institute of Biomedical Sciences, Hanyang University  );
  • 초록

    Calcium ions are implicated in a variety of physiological functions, including enzyme activity, membrane excitability, neurotransmitter release, and synaptic transmission, etc. Calcium antagonists have been known to be effective for the treatment of exertional angina and essential hypertension. Selective and nonselective voltage-dependent calcium channel blockers also have inhibitory action on the acute and tonic pain behaviors resulting from thermal stimulation, subcutaneous formalin injection and nerve injury. This study was undertaken to investigate the effects of iontophoretically applied $Ca^{++}$ and its antagonists on the responses of WDR (wide dynamic range) cells to sensory inputs. The responses of WDR cells to graded electrical stimulation of the afferent nerve and also to thermal stimulation of the receptive field were recorded before and after iontophoretical application of $Ca^{++}$ , EGTA, $Mn^{++}$ , verapamil, ${\omega}-conotoxin$ GVIA, ${\omega}-conotoxin$ MVIIC and ${\omega}-agatoxin$ IVA. Also studied were the effects of a few calcium antagonists on the C-fiber responses of WDR cells sensitized by subcutaneous injection of mustard oil (10%). Calcium ions and calcium channel antagonists ( $Mn^{++}$ , verapamil, ${\omega}-conotoxin$ GVIA & ${\omega}-agatoxin$ IVA) current-dependently suppressed the C-fiber responses of WDR cells without any significant effects on the A-fiber responses. But ${\omega}-conotoxin$ MVIIC did not have any inhibitory actions on the responses of WDR cell to A-fiber, C-fiber and thermal stimulation. Iontophoretically applied EGTA augmented the WDR cell responses to C-fiber and thermal stimulations while spinal application of EGTA for about $20{\sim}30\;min$ strongly inhibited the C-fiber responses. The augmenting and the inhibitory actions of EGTA were blocked by calcium ions. The WDR cell responses to thermal stimulation of the receptive field were reduced by iontophoretical application of $Ca^{++}$ , verapamil, ${\omega}-agatoxin$ IVA, and ${\omega}-conotoxin$ GVIA but not by ${\omega}-conotoxin$ MVIIC. The responses of WDR cells to C-fiber stimulation were augmented after subcutaneous injection of mustard oil (10%, 0.15 ml) into the receptive field and these sensitized C-fiber responses were strongly suppressed by iontophoretically applied $Ca^{++}$ , verapamil, ${\omega}-conotoxin$ GVIA and ${\omega}-agatoxin$ IVA. These experimental findings suggest that in the rat spinal cord, L-, N-, and P-type, but not Q-type, voltage-sensitive calcium channels are implicated in the calcium antagonist-induced inhibition of the normal and the sensitized responses of WDR cells to C-fiber and thermal stimulation, and that the suppressive effect of calcium and augmenting action of EGTA on WDR cell responses are due to changes in excitability of the cell.


  • 주제어

    Normal and sensitized WDR cell .   C fiber responses .   Thermal response .   Calcium channel antagonist .   Iontophoresis.  

 저자의 다른 논문

  • 강석한 (6)

    1. 1989 "피부염에 의해 유발된 척수후각세포의 Activity 변동에 관한 연구" 대한생리학회지 = Korean journal of physiology 23 (1): 151~167    
    2. 1989 "암모니아가 가토심방근 및 동방결절세포의 활동전압에 미치는 효과" 대한생리학회지 = Korean journal of physiology 23 (2): 339~350    
    3. 1990 "Taurine 및 ${\beta}-alanine$이 고양이 척수후각세포의 Activity에 미치는 효과" 대한생리학회지 = Korean journal of physiology 24 (1): 171~180    
    4. 1999 "Calcium Modulates Excitatory Amino Acid (EAA)- and Substance P-induced Rat Dorsal Horn Cell Responses" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 3 (1): 35~45    
    5. 2000 "Sprague-Dawley계 정상 흰쥐에서 위점막 알코올 탈수소효소 활성에 대한 성별의 영향" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 8 (1): 38~43    
    6. 2002 "Involvement of NMDA Receptor and L-type Calcium Channel in the Excitatory Action of Morphine" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 6 (5): 241~246    
  • 김기순 (35)

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