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흰쥐 해마절편에서 포도당/산소 고갈에 의한 5-hydroxytryptamine 유리변동에 미치는 Adenosine의 영향
Effect of Adenosine on the Release of $[^3H]-5-hydroxytryptamine$ during Glucose/Oxygen Deprivation from Rat Hippocampal Slices

차광은   (이화여자대학교 의과대학 약리학교실UU0001056  ); 배영숙   (이화여자대학교 의과대학 약리학교실UU0001056  ); 이경은   (이화여자대학교 의과대학 약리학교실UU0001056  );
  • 초록

    The effects of adenosine, adenosine A1 receptor antagonist (DPCPX), or NMDA receptor antagonist (APV) on the spontaneous release of $[^3H]-5-hydroxytryptamine$ ( $[^3H]-5-HT$ ) during normoxic/normoglycemic or hypoxic/hypoglycemic period were studied in the rat hippocampal slices. The hippocampus was obtained from the rat brain and sliced $400\;{\mu}m$ thickness with the tissue slicer. After 30 min's preincubation in the normal buffer, the slices were incubated for 30 min in a buffer containing $[^3H]-5-HT$ ( $0.1\;{\mu}M,\;74{\mu}Ci/8\;ml$ ) for uptake, and washed. To measure the release of $[^3H]-5-HT$ into the buffer, the incubation medium was drained off and refilled every ten minutes through sequence of 14 tubes. Induction of glucose/oxygen deprivation (GOD; medium depleting glucose and gassed with 95% $N_2/5%\;CO_2$ ) was done in 6th and 7th tube. The radioactivities in each buffer and the tissue were counted using liquid scintillation counter and the results were expressed as a percentage of the total radioactivities. When slices were exposed to GOD for 20 mins, the spontaneous release of $[^3H]-5-HT$ was markedly increased and this increase of $[^3H]-5-HT$ release was blocked by adenosine ( $10\;{\mu}M$ ) or DL-2-amino-5-phosphonovaleric acid (APV; $30\;{\mu}M$ ). Adenosine $A_1$ receptor specific antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) exacerbate GOD-induced increase of spontaneous release of $[^3H]-5-HT$ . These results suggest that Adenosine may play a role in the GOD-induced spontaneous release of $[^3H]-5-HT$ through adenosine $A_1$ receptor activity.


  • 주제어

    Adenosine .   glucose/oxygen deprevation .   hippocampal slice .   $[^3H]-5-hydroxytryptamine$ release.  

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    2. 1996 "니코틴의 마우스 소뇌과립세포내 칼슘의 항상성 조절기전" The Korean journal of pharmacology : official journal of the Society of Pharmacology, Republic of Korea 32 (1): 13~21    
    3. 1996 "Methanol이 배양된 흰쥐 해마의 신경세포 및 신경교 세포의 성장에 미치는 영향" 한국독성학회지 = The Korean journal of toxicology 12 (2): 203~211    
    4. 1997 "흰쥐 해마 절편에서 저산소증에 의한 [$^3H$-5-Hydroxytrytamine의 유리 변동에 미치는 superoxide dismutase/catalase의 영향" 한국독성학회지 = The Korean journal of toxicology 13 (4): 359~365    
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  • Lee, Kyung Eun (13)

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