본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Mouse Leukemia 세포에서 Adenosine 5'-triphosphate에 의한 Apoptosis
Apoptosis Induced by Adenosine 5'-triphosphate in Mouse Leukemic Cells

주난영   (연세대학교 원주의과대학 생리학교실UU0000936  ); 박규상   (연세대학교 원주의과대학 생리학교실UU0000936  ); 정해숙   (연세대학교 원주의과대학 생리학교실UU0000936  ); 공인덕   (연세대학교 원주의과대학 생리학교실UU0000936  ); 이중우   (연세대학교 원주의과대학 생리학교실UU0000936  );
  • 초록

    Extracellular ATP elicits various biological responses and plays a significant role in physiological regulation. Recently, ATP-induced growth inhibitions were reported in some tumor cell lines, but these effects and mechanisms are not well hewn. This study was conducted to investigate ATP-induced growth inhibition in mouse $leukemic(P388D_1)$ cells. ATP inhibited cell growth in a dose-dependent manner as analyzed by MTS assay $(IC_{50}: 33.1\;{\mu}M)$ . Nucleotides other than ATP, such as ADP $(37.5;{\mu}M)$ and AMP $(33.2;{\mu}M)$ had the same effects as ATP but adenosine $(57.8;{\mu}M)$ showed less effect than ATP. ATP attenuated the cells in $G_0/G_l\;and\;G_2/M$ phases but increased those in S phase in flow cytometric analysis. Hypodiploid cells $(A_0)$ , the presumptive findings of apoptosis, were found among the ATP-treated cells. ATP induced DNA fragmentation into $180{\mu}200\;bps $ as measured by electrophoresis. some apoptotic cells were stained by TUNEL method. ATP increased the intracellular free $Ca^{++}$ concentration $([Ca^{++}]_i)$ and the increment of $([Ca^{++}]_i)$ was caused by influx from the extracellular space. These results suggest that extracellular ATP induces growth inhibition through apoptosis.


  • 주제어

    Adenosine 5'-triphosphate(ATP) .   Leukemic cells .   Apoptosis .   Intracellular free calcium concentration $([Ca^{++}]i)$.  

 저자의 다른 논문

  • Park, Kyu-Sang (7)

    1. 1999 "Effects of Protein Kinase C Modulation on Hepatic Hemodynamics and Glucoregulation" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 3 (6): 571~578    
    2. 1999 "Depression of L-type $Ca^{2+}$ and Transient Outward $K^+$ Currents in Endotoxin-treated Rat Cardiac" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 3 (6): 623~630    
    3. 2002 "Identification of ATP-sensitive $K^+$ Conductances in Male Rat Major Pelvic Ganglion Neurons" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 6 (5): 247~253    
    4. 2002 "Modulation of Inwardly Rectifying $K^+$ Channel by Intracellular and Extracellular pH in Bovine Aortic Endothelial Cells" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 6 (5): 255~260    
    5. 2003 "제대정맥 내피세포에서 Amiloride 유도체에 의한 Apoptosis 반복" 소아과 = Journal of the Korean pediatric society 46 (1): 56~66    
    6. 2004 "Characterization of Acetylcholine-induced Currents in Male Rat Pelvic Ganglion Neurons" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 8 (4): 219~225    
    7. 2007 "Regulation of Nicotinic Acetylcholine Receptor by Tyrosine Kinase in Autonomic Major Pelvic Ganglion Neurons" Journal of experimental & biomedical sciences = 대한의생명과학회지 13 (2): 119~125    
  • 정해숙 (1)

  • 공인덕 (13)

  • 이중우 (15)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 대한약리학회 : 저널
  • 대한생리학회 : 저널
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기