본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

한국전통 식품의 원료인 메주와 누룩에서 분리된 황곡균에 대한 연구
Studies on the Yellow Fungal Isolates (Aspergillus species) Inhabiting at the Cereals in Korea

이상선   (한국교원대학교 대학원UU0001379  ); 박대호   (한국교원대학교 대학원UU0001379  ); 성창근   (충남대학교 식품공학과UU0001302  ); 유진영   (한국식품개발원 생물공학부CC0186878  );
  • 초록

    The yellow fungal isolates inhabiting at the cereals (Hwang-Kuk, HK-fungi) were widely collected from the mejus and nuluks in Korea; the meju is a raw material for Korean traditional foods for soysauce and soypaste, and the nuluk is a raw material for Korean traditional rice wine. These isolates, well known as an Aspergillus oryzae producing amylase for Korean rice wine or producing protease for soybeans, were compared with Aspergillus species known. All isolates were microscopically observed to be a species of A. oryzae or its related, but to be difficult to be identified. Thus, RAPD-DNA techniques were applied for these isolates and analyzed with nummerical values using NT-system, or Ecological programs or Factorial analyses. Several common bands of RAPD-DNA in the 28 isolates were synthesized with the different OPD primers and speculated to be used for identification of HK fungi. The HK-fungi isolated were revealed to belong to the group of A. flavus previously defined. Particularly, the isolates collected from mejus were analyzed to be more closed to A. flavus, The species of A. flavus, A. oryzae and A. sojae were grouped at the values lower than those indicating the diversity of species. In other words, these three fungal species were not distinguishable and all isolates known as a HK-fungus were very closed to A. flavus, All isolates were not diversified at groupings of RAPD-DNA, and considered to be not the natural flora at the mejus or nuluks. The meju or nuluk having the above fungi as the fungal flora were speculated to be not termed "Korean traditional foodstuffs".The yellow fungal isolates inhabiting at the cereals (Hwang-Kuk, HK-fungi) were widely collected from the mejus and nuluks in Korea; the meju is a raw material for Korean traditional foods for soysauce and soypaste, and the nuluk is a raw material for Korean traditional rice wine. These isolates, well known as an Aspergillus oryzae producing amylase for Korean rice wine or producing protease for soybeans, were compared with Aspergillus species known. All isolates were microscopically observed to be a species of A. oryzae or its related, but to be difficult to be identified. Thus, RAPD-DNA techniques were applied for these isolates and analyzed with nummerical values using NT-system, or Ecological programs or Factorial analyses. Several common bands of RAPD-DNA in the 28 isolates were synthesized with the different OPD primers and speculated to be used for identification of HK fungi. The HK-fungi isolated were revealed to belong to the group of A. flavus previously defined. Particularly, the isolates collected from mejus were analyzed to be more closed to A. flavus, The species of A. flavus, A. oryzae and A. sojae were grouped at the values lower than those indicating the diversity of species. In other words, these three fungal species were not distinguishable and all isolates known as a HK-fungus were very closed to A. flavus. All isolates were not diversified at groupings of RAPD-DNA, and considered to be not the natural flora at the mejus or nuluks. The meju or nuluk having the above fungi as the fungal flora were speculated to be not termed 'Korean traditional foodstuffs'.


  • 주제어

    A.   flavus .   A.   oryzae .   A.   sojae .   RAPD bands .   fermented soybean .   Korean wine.  

  • 참고문헌 (21)

    1. 식품미생물학 , 김찬조;장지현 , / v.,pp.76-81,
    2. 재래 메주 발효과정 중 나타나는 미생물에 대한 연구 , 박광호 , / v.,pp.,
    3. 전통발효식품의 과학과 연구, 전통혼성 주의 품질 향상 및 산업화 기술연구 , 오만진 , / v.,pp.,
    4. 전통메주에 관련된 미생물의 생물학적인 분류 및 안정성 연구(위탁-5);전통 장류용 메주의 산업화를 위한 기반기술 연구(전통발효식품의 과학화 연구) , 이상선 , / v.,pp.393-464,
    5. RAPD분석법에 의한 소 품종 판별용 표지 인자의 검출 최적화 연구 , 이창수;상병찬 , 한국식품학회지 / v.15,pp.35-39,
    6. 한국 재래식 간장의 발효 미생물에 관한 연구 , 조덕현;이우진 , 한구농화학회지 / v.13,pp.35-42,
    7. Introductory Mycology , Alexopoulos, C.J.;Mims, C.W.;Blackwell, M. , / v.,pp.309,
    8. Methods in microbiology, Vol. 4 , Boothe, C. , / v.,pp.,
    9. DNA relatedness among wild and domesticated species in the Aspergillus flavus group , Kurtzman, C.P.;Smiley, M.J.;Robnett, C.J.;Wicklow, D.T. , Mycologia / v.78,pp.955-959,
    10. Identification and Isolation of Zygomyceteous fungi found on Maeju, a Raw Material of korean Traditional soysauces , Lee, S.S.;Park, K.H.;Choi, K.J.;Won, S.A. , Kor. J. Mycol. / v.21,pp.172-187,
    11. Meju fermentation for a Raw material of Korean traditional Soy-sauce , Lee, S.S. , Kor. J. Mycol. / v.23,pp.161-175,
    12. Statistical Ecology , Ludwig, J.A.;Reynolds, J.F. , / v.,pp.165-202,
    13. The Genus Aspergillus , Powell, K.A.;Renwick, A.;Peberdy, J.F. , / v.,pp.1188,
    14. The genus Aspergillus , Raper, K.B.;Fennell, D.I. , / v.,pp.,
    15. Numerical analysis , Rohlf, F.;Kishpaugh, J.;Kirk, D. , / v.,pp.,
    16. Moleular cloning-A Laboratory manual , Sambrook, J.;Fritsch, E.F.;Maniatic, T. , / v.,pp.6.1-6.19,
    17. Biotechnology Handbook 7 , Smith, J.E. , / v.,pp.1-18,
    18. DNA Fingerprinting in Plants and Fungi , Weising, K,;Nybom, H.;Wolff, K.;Meyer, W. , / v.,pp.24-135,
    19. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers , Williams, J.G.K.;Kubelik, A.R.;Livak, K.J.;Rafalski, J.A.;Tingey, S.V. , Nucl. Acids Res. / v.18,pp.6531-6535,
    20. Electrophoretic comparison of enzymes as a chemotaxonomic acid among Aspergillus taxa (2). Aspergillus sect. Flavi.;Mordern Concepts of Penicillium and Aspergillus Classification , Yamatoya, T.;Sugiyama, J.;Kuraishi, H.;Samson, R.A.(ed.);Pitt, J.I.(ed.) , / v.,pp.395-406,
    21. Studies on the fungal isolates of Mucorales collected from Korean home made mejus and nuluks , Yu, K.W.;Seong, C.K.;Lee, S.S.;Yoo, J.Y. , Kor. J. Mycol. / v.24,pp.280-292,

 저자의 다른 논문

  • Lee, Sang-Sun (77)

    1. 1987 "Helminthosporium sativum가 생성하는 독소물질에 대한 phytotoxicity 및 Dilution end-Points 측정 방법 개발" 한국식물병리학회지 = Korean journal of plant pathology 3 (3): 198~202    
    2. 1988 "H. sativum이 생성(生成)하는 식물(植物) 독소물질 분리(分離)" 한국균학회지 = The Korean journal of mycology 16 (1): 9~15    
    3. 1989 "산림 및 해안지역에서 발견된 내생균근" 한국균학회지 = The Korean journal of mycology 17 (1): 14~20    
    4. 1989 "한국에서 시판되는 Agaricus bisporus 의 담자기 형태" 한국균학회지 = The Korean journal of mycology 17 (1): 48~50    
    5. 1990 "Fructosyl transferase를 생성하는 효모의 분리 및 동정" 한국균학회지 = The Korean journal of mycology 18 (1): 20~25    
    6. 1990 "고마리 군락의 토양에서 발견된 내생균근" 한국균학회지 = The Korean journal of mycology 18 (1): 26~41    
    7. 1990 "한국에서 발견된 Complexipes moniliformis 종(種)의 다양성(多樣性)" 한국균학회지 = The Korean journal of mycology 18 (3): 127~131    
    8. 1990 "한국에서 발견된 Acaulospora scrobiculata의 재기재(再記載)" 한국균학회지 = The Korean journal of mycology 18 (3): 178~180    
    9. 1990 "식물(植物) 군락(群落)에서 VA 내생균근(內生菌根) 발견(發見)" 한국균학회지 = The Korean journal of mycology 18 (4): 191~197    
    10. 1991 "프탈산 화합물을 탄소원으로 이용하는 효모의 분리 및 동정" 한국균학회지 = The Korean journal of mycology 19 (1): 41~46    
  • Yoo, Jin-Young (47)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기