본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Natural product sciences v.3 no.1, 1997년, pp.19 - 28  

Detection of Antiinflammatory Agents from Natural Products as Inhibitors of Cyclooxygenase I and II

Lee, Dong-Hee   (Natural Products Research Institute, Seoul National UniversityUU0000691  ); Kang, Sam-Sik   (Natural Products Research Institute, Seoul National UniversityUU0000691  ); Chang, Il-Moo   (Natural Products Research Institute, Seoul National UniversityUU0000691  ); Mar, Woong-Chon   (Natural Products Research Institute, Seoul National UniversityUU0000691  );
  • 초록

    Constitutive cyclooxygenase (COX-I) is present in cells under physiological conditions, whereas inducible cyclooxygenase (COX-II) is induced by some cytokines, mitogens, and endotoxin presumably in pathological conditions such as inflammation. We have evaluated the inhibitory effects of solvent fractionated extracts of natural products on the activities of COX-I and COX-II. Oxygen uptake COX assay was performed, as a primary screening from the tissue extracts of bovine seminal vesicles (BSV), by monitoring the initial rate of oxygen uptake using an oxygen electrode. Additionally, we evaluated plant extracts for the inhibitory effects of COX-I (in HEL cells) and COX-II (in lipopolysaccharide activated J774A.1 macrophages) using thin layer chromatography of prostanoids produced from $^{14}C-labelled$ arachidonic acid (AA). The use of such models of COX-I and COX-II assay will lead to the identification of specific inhibitors of cyclooxygenases with presumably less side effects than present therapies. Inhibitory effects of 50 kinds of plant extracts on the COX-I and COX-II activities were determined and the active fractions were found in the ethyl acetate fractions of Dryopteris crassirhizoma (roots), Amomum cardamomum (roots), Triticum aestivum (seeds), Perilla sikokiana (leaves), Anemarrhena asphodeloides (roots). Especially, the ethyl acetate fraction of Dryopteris crassirhizoma (roots), which exhibited the strong inhibition against BSV COX $(IC_{50},\;65.4\;{\mu}g/ml)$ , COX-I $(IC_{50},\;8.5\;{\mu}g/ml)$ , and COX-II $(IC_{50},\;17.2\;{\mu}g/ml)$ , is under investigation to isolate active principles using activity-guided fractionation method.


  • 주제어

    Cyclooxygenase I .   Cyclooxygenase II .   Inflammation .   Antiinflammation .   HEL cells .   J774A.   1 cells .   Arachidonic acid .   Lipopolysaccharide.  

 저자의 다른 논문

  • 강삼식 (212)

    1. 1973 "Sterols and terpenoids from phytolacca esculenta" 약학회지 = Yakhak hoeji 17 (3): 152~160    
    2. 1973 "Phytosterolins from phytolacca esculenta" 약학회지 = Yakhak hoeji 17 (3): 161~166    
    3. 1974 "상륙(商陸)의 Pentacyclic Triterpenoid" 생약학회지 5 (1): 69~79    
    4. 1974 "상륙(商陸)의 신(新) 성분(成分) Phytolaccagenic Acid에 관한 연구" 생약학회지 5 (2): 125~127    
    5. 1974 "Glucolipid from Phytolacca esculenta" 약학회지 = Yakhak hoeji 18 (3): 203~208    
    6. 1974 "Nature of the Hydrogen Chloride Induced Isomers of $\alpha$-Spinasterol" 약학회지 = Yakhak hoeji 18 (3): 217~227    
    7. 1974 "The Structure and Stereochemistry of Phytolaccagenic Acid" 약학회지 = Yakhak hoeji 18 (4): 231~235    
    8. 1975 "3-Oxo-30-carbomethoxy-24-norolean-12-en-28-oic Acid, a Nortriterpene Artifact from Phytolacca americana" 약학회지 = Yakhak hoeji 19 (1): 9~15    
    9. 1975 "The Occurrence and Chemistry of Phytolacca Triterpenoids" 약학회지 = Yakhak hoeji 19 (3): 189~208    
    10. 1975 "Sterols and Triterpenoids from Codonopsis lanceolata" 약학회지 = Yakhak hoeji 19 (3): 209~213    
  • Chang, Il-Moo (52)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 한국생약학회 : 저널
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기