본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

STRUCTURAL PERTURBATIONS INDUCED BY PHOTODYNAMIC ACTION OF PORPHYRIN AGGREGATES ON PLASMA MEMBRANE AND MICROSOMES OF GLIOBLASTOMA CELLS

Sreentvasan, Rajesh    (Department of Biophysics, National Institute of Mental Health and Neurosciences   ); Joshi, Preeti G.    (Department of Biophysics, National Institute of Mental Health and Neurosciences   ); Joshi, Nanda B.    (Department of Biophysics, National Institute of Mental Health and Neurosciences  );
  • 초록

    The plasma membrane and microsomes, isolated from the cells treated with hematoporphyrm derivative (HpD) for 1 and 24 h, accumulated the aggregated porphyrin. The quantity of aggregated porphyrin was same in the plasma membrane and microsomes after isolating them from cells treated with HpD for 1 h whereas the microsomes accumulated higher quantity of aggregated porphyrin when cells were treated with HpD for 24 h. Photodynamic action of aggregated porphyrin on plasma membrane and microsomes was investigated using lipid specific fluorescent probes: 1,6-diphenyl-1,3,5-hexatrine (DPH) and 1-(4-trimethylammonium), 6-diphenyl-1,3,5-hexatrine(TMA-DPH). The time dependent anisotropy of these probes in the membranes was measured and the decay of anisotropy was analyzed using wobbling in cone model. Upon irradiation both the plasma membrane and the microsomes showed an increase in the limiting anis~)tropy and order parameter and a decrease in the cone angle of the lipid probes. The increase in the limiting anisotropy was pronounced in membranes isolated from the cells treated with HpD for 24 h. Photoinduced change in the limiting anisotropy was dependent on the duration of incubation of cells with HpD before isolating the membranes. In both the membranes. the membrane core was affected more as compared to the outer leaflet. In addition to the structural changes, a decrease in Na$^+$-K$^+$-ATPase and NADPH cyt c reductase activity was also observed upon irradiation of HpD treated cells. Inhibition in NADPH cyt c reductase was more when cells were treated with HpD for 24 h, however, Na$^+$-K$^+$-ATPase activity did not depend on the duration of the treatment of cells with HpD before irradiation. Our results suggest that the extent of photoinduced perturbations in the membranes varies as a function of duration of the treatment of cells with HpD and the membrane core is more susceptible to the photodynamic action of aggregated porphyrin.


  • 참고문헌 (32)

    1. Photodynamic therapy of tumors and other diseases using porphyrins , Spikes, J. D.;G. Jori , Lasers Med. Sci. / v.2;3,pp.3-15,
    2. Sites of photosensitization by derivative of hematoporphyrin , Kessel, D. , Photochem. Photobiol. / v.46,pp.489-493,
    3. $Ca^{2+}$ influx induced by photodynamic action in human cerebral glioma cells: possible involvement of a calcium channel , Joshi, P. G.;K. Joshi;S. Mishra;N. B. Joshi , Photochem. Photobiol. / v.60,pp.244-248,
    4. Influence of immune complexes on macrophase membrane fluidity: a nanosecond fluorescence anisotropy study , Petty, H. R.;C. D. Neibylski;J. W. Francis , Biochemistry / v.26,pp.6340-6348,
    5. Lakowicz, J. R. , Principles of Fluorescence Spectroscopy / v.,pp.,
    6. Nanosecond fluorescence anisotropy decays of 1,6-diphenyl-1,3,5-hexatrine in membranes , Hildenbrand, K.;C. Nicolan , Biochem. Biophys. Acta / v.553,pp.365-377,
    7. Binding of hematoporphyrin derivative to brain tumor cells- a fluorescence spectroscopic study , Joshi, K.;P. G. Joshi;N. B. Joshi , Photochem. Photobiol. / v.56,pp.113-118,
    8. Photochemotherapy of cancerexperimental research , Moan, J.;K. Berg , Photochem. Photobiol. / v.55,pp.931-948,
    9. Microviscosity parameters and protein mobility in biological membranes , Shintzsky, M.;M. Inbar , Biochem. Biophys. Acta / v.433,pp.133-149,
    10. Dynamic structure of biological membranes as probed by 1,6-diphenyl-1,3,5-hexatrine: a nanosecond fluorescence depolarization study , Kinosita, K.;K. Kataoka;Y. Kimura;O. Gotoh;R. Ekegami , Biochemistry / v.20,pp.4270-4277,
    11. Structural alterations induced by photodynamic action of hematoporphyrin derivative in plasma membrane of glioblastoma cells: time dependent fluorescence spectroscopic study , Joshi, K.;P. G. Joshi;N. B. Joshi , Ind. J. Biochem. Biophys. / v.32,pp.200-206,
    12. Antiviral activity of merocyanine 540 , Sieber, F.;J. M. O'Brien;G. J. Krueger;S. L. Schober;W. H. Burns;S. J. Sharkis;L. L. Sensenbrenner , Photochem. Photobiol. / v.46,pp.707-711,
    13. Protoporphyrin induced photodynamic effect on band-3 protein of human erythrocyte membranes , Dubbelman, T. M. A. R.;A. F. P. M. DeGoeij;K. Christiansen;J. Van Stevninck , Biochem. Biophys. Acta / v.649,pp.310-316,
    14. Hematoporphyrin derivative binding and photosensitization in human glioblastoma cells: comparison of exponential and plateau phase cells , Sreenivasan, R.;P. G. Joshi;N. B. Joshi , Ind. J. Expt. Biol. / v.32,pp.763-766,
    15. Reactions of radicals with lecithin bilayers , Barber, D. J. W.;J. K. Thomas , Rad. Res. / v.75,pp.51-65,
    16. Photosensitizing effect of hematoporphyrin derivative and photofrin Ⅱ on plasma membrane enzymes 5¹-nucleotide, $Na^+$-$K^+$-ATPase and $Mg^{2+}$-ATPase in R3230 AC mammary adenocarcinoma , Gibson, S. L.;R. S. Murant;R. Hilf , Cancer Res. / v.48,pp.3360-3366,
    17. Hematoporphyrin derivative (photofrin) photodynamic action on $Ca^{2+}$ transport in monkey kidney cells (CV-1) , Dellinger, M.;F. Ricchelli;G. Moreno;C. Salet , Photochem. Photobiol. / v.60,pp.368-372,
    18. Hematoporphyrin derivative induced photodamage to brain tumor cells: alteration in subcellular membranes , Sreenivasan, R.;P. G. Joshi;N. B. Joshi , Rad. Phys. Chem. / v.49,pp.145-149,
    19. 1-(4-Trimethylammonium), 6-diphenyl-1,3,5-hexatrine synthesis, fluorescence properties and use as a a fluorescent probes of lipid bilayers , Prendergast, G.;R. Haugland;P. J. Callahan , Biochemistry / v.20,pp.7333-7338,
    20. The increase of phospholipid bilayer rigidity after lipid peroxidation , Dobretsov, G. E.;T. A. Borscheievskaya;V. A. Petrov;Y. A. Vladimirov , FEBS Letts. / v.84,pp.125-128,
    21. Differential role of reactive oxygen intermediates in photofrin Ⅰ and photofrin Ⅱ mediated photoenhancement of lipid peroxidation in epidermal microsomal membrane , Athar, M.;H. Muktar;D. R. Bickers , J. Invest. Dermatol. / v.90,pp.652-657,
    22. Photoradiation therapy , Dougherty, T. J. , Photochem. Photobiol. / v.58,pp.895-900,
    23. Subcellular fractionation of cultured glial cells , Cotman, C.;H. Herschman;D. Taylor , J. Neurobiol. / v.2,pp.169-180,
    24. Evaluation of membrane fluidity effects and enzyme activity alterations in adriamycin neurotoxicity , Deliconstantinos, G.;K. T. Luidmila;V. Vassilik , Biochem. Pharma. / v.36,pp.1153-1161,
    25. Plasma membrane depolarization and calcium influx during cell injury by photodynamic action , Specht, K. G.;M. A. J. Rodgers , Biochem. Biophys. Acta / v.1070,pp.60-68,
    26. Retention and photodynamic effects of hematoporphyrin derivative in cells after prolonged cultivation in the presence of porphyrins , Christensen, T.;T. Sandquist;K. Feven;H. Wakshvik;J. Moan , Br. J. Cancer / v.48,pp.35-43,
    27. A theory of fluorescence polarization decay in membranes , Kinosita, K.;S. Kawato;A. Ikegami , Biophys. J. / v.20,pp.289-305,
    28. Photobiochemistry of porphyrins , Jori, G.;J. D. Spikes;K. C. Smith(ed.) , Topics in Photomedicine / v.,pp.183-318,
    29. Photomodification of biological membranes with emphasis on singlet oxygen mechanism , Valenzeno, D. P. , Photochem. Photobiol. / v.46,pp.147-160,
    30. Microsomal enzymes involved in toxicity-analysis and separation , Guengerich, P. P.;A. W. Hayes(ed.) , Principles and Methods of Toxicology / v.,pp.609-634,
    31. Photoradiation therapy and its potential in the management of neurological tumors , Kaye, A. H.;G. Morstyn;M. L. J. Apuzzo , J. Neurosurg. / v.69,pp.1-14,
    32. Determination of $^3H$ and $^{14}C$ HpD distribution in malignant and normal tissue , Gomer, C.;T. J. Dougherty , Cancer Res. / v.39,pp.146-151,

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기