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Pentachlorophenol 대사물과 세포내 거대분자물의 반응에 관한 연구
Covalent Interactions of Reactive Pentachlorophenol Metabolites with Cellular Macromolecules

정요찬    (서울대학교 수의과대학   ); 윤병수    (경기대 이과대학   ); 이영순    (서울대학교 수의과대학   ); 조명행    (서울대학교 수의과대학  );
  • 초록

    Pentachlorophenol(PCP) which ks widely used in wood preservation, pulp and paper mills, has led to a substantial envirortmental contamination. To get the reliable data for the effective health risk assessment with PCP, covalent binding potential of PCP to cellular macromolecules and glutathione(GSH) was investigated after intraperitoneal administration of $^{14}C-PCP$ to rats. PCP metabolites were able to bind covalently to serum albumin and hepatic protein in a dose- and time-dependent manner. Hepatic protein adducts of PCP metabolites were increased as a function of cytochrome P-450 activities, whereas, albumin adducts significantly decreased. Covalent binding of PCP metabolites with DNA or hemoglobin was not observed. GSH levels in liver tissue decreased over 12hrs, however, the level was recovered after 48hrs. Tetrachloro-1,4-benzoquinone (1,4-TCBQ), one of the most reactive PCP metabolites, conjugated with GSH very rapidly. Base on our results, we could conclude that PCP metabolized to reactive electrophilic metabolites by cytochrome P-450 isoenzymes and conjugated rapidly with neighboring protein or nonprotein sulfhydryl before reacting with DNA or hemoglobin. We propose that albumin adducts and mercapturic acids of PCP metabolites can be used good biomarker of recent PCP exposure.


  • 주제어

    Pentachlorophenol .   biomarker .   albumin adduct .   reactive metabolites.  

  • 참고문헌 (27)

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