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The journal of microbiology v.35 no.1, 1997년, pp.15 - 20  

Catalytic mechanism and inhibition studies of purine nucleoside phosphorylase (PNP) in micrococcus luteus

Choi, Hye-Seon    (Department of Microbiology, Ulsan University  );
  • 초록

    Kinetic studies were done to elucidate the reaction mechanism of purine nucleoside phosphorylase (PNP) in Micrococcus Luteus. PNP catalyzes the reversible phosphorolysis of ribonucleosides to their respective base. The effect of alternative competing substrates suggested that a single enzyme was involved in binding to the active site for all purine nucleosides, inosine, deoxyiosine, guanosine, deoxyguanosine, adenosine and deoxyadenosine. Affinity studies showed that pentose moiety reduced the binding capacity and methylation of ring N-1 of inosine and guanosine had little effect on binding to bacterial enzyme, whereas these compounds did not bind to the mammalian enzymes. The initial velocity and product inhibition studies demonstrated that the predominant mechanism of reaction was an ordered bi, bi reaction. The nucleoside bound to the enzyme first, followed by phosphate. Ribose 1-phosphate was the first product to leave, followed by base.


  • 주제어

    Purine nucleside phosphoryalse.   Reaction mechanism .   Micrococcus luteus.  

  • 참고문헌 (29)

    1. Three-dimensional structure of human erythroeytic purine nucleoside phosphorylase at 3.2 A resolution , Ealick, S. E.;Rule, S.A.;Carter, D.C.;Greenhough, T.J.;Babu, Y.S.;Cook, W.J.;Habash, J.;Helliwell, J.R.;Stoeckler, J.D.;Parks, R.E., Jr.;Chen, S.F.;Bugg, C.E. , J. Biol. Chem. / v.265,pp.1812-1820,
    2. Purine nucleoside phosphorylase from bovine liver , Ikezawa, Z.;Nishino, T.;Murakami, K.;Tsushima, K. , Comp. Biochem. Physiol. / v.60B,pp.111-116,
    3. Rabbit brain purine nucleoside phosphorylase , Lewis, A.S. , Arch. Biochem. Biophys. / v.190,pp.662-670,
    4. Selective modulation of antibody response and natural killer cell activity by purine nucleoside analogeus , Pribe, T.;Kandil, O.;Nakic, M.;Pan, B.F.;Nelson, J.A. , Cancer Res. / v.48,pp.4799-4803,
    5. Allosteric regulation of purine nucleoside phosphorylase , Ropp, P.A.;Traut, T.W. , Arch. Biochem. Biophys. / v.288,pp.614-620,
    6. Inhibition of human purine nucleoside phosphorylase by acyclic nucleosides and nucleotides , Stein, J.M.;Stoeckler, J.D.;Li, S-Y.;Tolman, R.L.;MacCoss, M.;Chen, A.;Karkas, J.D.;Ashton, W.T.;Parks, R.E., Jr. , Biochem. Pharmacol. / v.36,pp.1237-1244,
    7. Partial purification and characterization of purine nuclesoside phosphorlase in Sacchromyces cerevisiae , Choi, H.S. , Kor. J. Microbiol. / v.29,pp.172-178,
    8. Purine nucleoside phosphorylase from Salmonella typhymurium and rat liver , Hoffee, P.A.;May, R.;Robertson, B.C.;P.A. Hoffee(ed);M.E. Jones(ed) , Methods in Enzymology / v.51,pp.517-524,
    9. Depressed activities of purine enzymes in lymphocytes of patients infected with human immunodeficiency virus , Renout, J.A.;Wood, A.;Frazer, I.H.;Thong, Y.H.;Chalmer, A.H. , Clin. Chem. / v.35,pp.1478-1481,
    10. Selective in vitro inhibition of human molt-4 lymphoblasts by the novel purine nucleoside phosphorylase inhibitor, CI-972 , Gilbertsen, R.B.;Dong, K.K.;Kossarek, L.M.;Sircar, J.C.;Kostlan, C.R.;Conroy, M.C. , Biochem. Biophys. Res. Commun. / v.178,pp.1351-1358,
    11. Purification and characterization of a novel nucleoside phosphorylase from a Klebsiella sp. and its use in the enzymatic production of adenine arabinoside , Ling, F.;Inou, Y.;kimura, A. , Appl. Environment. Microbiol. / v.56,pp.3830-3834,
    12. Purification and characterization of purine nucleoside phosphorylase in Micrococcus luteus , Choi, H.S. , Kor. J. Microbiol. / v.34,pp.82-89,
         
    13. Biochemistry of diseases of immunodevelopment , Martin, D.W., Jr.;Gelfand, E.W. , Ann. Rev. Biochem. / v.50,pp.845-877,
    14. Nucleoside kinases in T and B lymphoblasts distinguished by autoradiography , Osborne, W.R, , Proc. Natl. Acad. Sci. USA / v.83,pp.4030-4034,
    15. The biochemistry and pharmacology of PD 116124 (8-amino-2'-nordeoxyguanosine), an inhibitor of purine nucleoside phosphorylase (PNP) , Dong, M.K.;Scott, M.E.;Schrier, D.J.;Suto, M.J.;Sircar, J.C.;Black, A.;Chang, T.;Gilbertsen, R.B. , J. Pharmacol. Exp. Ther. / v.260,pp.319-326,
    16. Two purine nucleoside phosphorylases in Bacillus subtilis , Jensen, K.F. , Biochim. Biophys. Acta / v.525,pp.346-356,
    17. Crystallization and preliminary X-ray invetigation of human erythroeytic purine nucleoside phosphorylase , Cook, W.J.;Ealick, S.E.;Bugg, C.E.;Stoeckler, J.D.;Parks, R.E., Jr , J. Biol. Chem. / v.256,pp.4079-4080,
    18. Application of crystallographic and modeling methods in the design of purine nucleoside phosphorylase inhibitors , Ealick, S.E.;Babu, Y.S.;Bugg, C.E.;Erion, M.D.;Guida, W.C.;Montgomery, J.A.;Secrist Ⅲ, J.A. , Proc. Natl. Acad. Sci. USA / v.88,pp.11540-11544,
    19. Kinetic analysis of purine nucleoside phosphrylase in Saccharomyces cerevisiae , Choi, H.S. , Kor. J. Microbiol. / v.31,pp.148-156,
         
    20. Purification and characterization of purine nucleoside phosphorylase from proteus vulgaris , Surette, M.;Gill, T.;MacLean, S. , Appl. Environment. Microbiol. / v.56,pp.1435-1439,
    21. Effects of acyalovir and its metabolites on purine nucleoside phosphorylase , Tuttle, J.V.;Krenitsky, T.A. , J. Biol. Chem. / v.259,pp.4065-4069,
    22. Crystallization and some properties of purine nucleoside phosphorylase from chicken liver , Murakami, K.;Tsushima, K. , Biochem. Biophys. Acta / v.384,pp.390-398,
    23. Inhibitors of purine nucleoside phosphorylase: effects of 9-deazapurine ribonucleosides and synthesis of 5'-deoxy-5'-iodo-9-deazainosine , Stoeckler, J.D.;Ryden, J.B.;Parks, R. E., Jr.;Chu, M.Y.;Lim, M.L.;Ren, W.Y.;Klein, R.S. , Cancer Res. / v.469,pp.1774-1778,
    24. Purine nucleoside phosphorylase , Bzowska, A.;Kulikowska, E.;Darzynkiewicz, E.;Shugar, D. , J. Biol. Chem. / v.263,pp.9212-9217,
    25. Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhymurium , Jensen, K.F. , Eur. J. Biochem. / v.61,pp.377-386,
    26. Differential spectrophotometry of purine compounds by means of specific enzymes , Kalckar, H.M. , J. Biol. Chem. / v.167,pp.429-443,
    27. Nucleoside analogue inhibitors of purine nucleoside phosphorylase , Krenitsky, T.A.;Tuttle, J.V.;Miller, W.H.;Moorman, A.R.;Orr, G.F.;Beauchamp, L. , J. Biol. Chem. / v.265,pp.3066-3069,
    28. Purine nucleoside phosphorylase , Stoeckler, J.D.;R.I. Glazer(ed) , Developments in Cancer Chemotherapy / v.,pp.35-60,
    29. IOsolation of adenosine-assimilating bacterium from soil: inducible production of purine nucleoside phosphorylase , Ling, F.;Inou, Y.;Kimura, A. , Agric. Biol. Chem. / v.55,pp.573-575,

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