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The journal of microbiology v.35 no.1, 1997년, pp.25 - 29   피인용횟수: 4

Reduction of hexavalent chromium by pseudomonas aeruginosa HP014

Oh, Young-Sook    (Hallym University   ); Choi, Sung-Chan    (Hallym University  );
  • 초록

    Microbial reduction of hexavalent (VI) to trivalent (III) chromium decreases its toxicity by two orders of magnitude. In order to investigate the nature of Cr-reduction, Cr-resistant Pseudomonas aeruginosa HP014 was isolated and tested for its reduction capability. At the concentration of 0.5 mM Cr(VI), cell growth was not inhibited by the presence of Cr(VI) in a liquid medium, and Cr(VI) reduction was accompanied by ell growth. When cell-free extract was tested, the reduction of Cr(VI) showed a saturation kinetics with the maximum specific activity of 0.33 .mu.mol min $^-1$ mg $^-1$ cell protein, and an apparent K. of 1.73 mM Cr(VI). The activity required either NADH or NADPH as an electron donor. However, NADPH gave 50% as mush activity as sequently the supernatant and pelleted membrane fractions were tested for Cr(VI) reduction activity. The supernatant of the centrifugation showed almost the same Cr(VI) reduction activity as compared with that of the cell-free extract, indicating that the Cr(VI)-reducing activity of P. aeruginosa HP-14 is due to soluble enzyme. Moreover, the activity appeared to be the highest among the known activities, suggesting that the strain might be useful for remediation of Cr(VI)-contaminated sites.


  • 주제어

    chromium .   reduction .   bioremediation.  

  • 참고문헌 (26)

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  • 이 논문을 인용한 문헌 (4)

    1. Bae, Woo-Chul ; Kang, Tae-Gu ; Jung, Jae-Han ; Park, Chul-Jae ; Choi, Sung-Chan ; Jeong, Byeong-Chul 2000. "Purification and Characterization of NADH-Dependent Cr(VI) Reductase from Escherichia coli ATCD33456" Journal of microbiology and biotechnology, 10(5): 580~586     
    2. 2000. "Simultaneous Removal of Phenol and Hexavalent Chromium by Rhodococcus sp. CP01" Korean journal of microbiology = 미생물학회지, 36(4): 279~284     
    3. 2000. "" The journal of microbiology, 38(1): 36~39     
    4. Jin, Tae-Eun ; Kim, Il-Gi ; Kim, Won-Sik ; Suh, Suk-Chul ; Kim, Byung-Dong ; Rhim, Seong-Lyul 2001. "Expression of Chromium (VI) Reductase Gene of Heavy Metal Reducing Bacteria in Tobacco Plants" Journal of plant biotechnology, 3(1): 13~17     

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