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The journal of microbiology v.35 no.1, 1997년, pp.53 - 60  

Improvement of 4-chlorobiphenyl degradation bya recombinant strain, pseudomonas sp. DJ12-C

Kim, Ji-Young    (Chungbuk National University   ); Kim, Young-Chang    (Chungbuk National University   ); You, Lim-Jai    (Chungbuk National University   ); Lee, Ki-Sung    (Pai Chai University   ); Ok, Ka-Jong    (Research Center for Molecular Microbiology, Seoul National University   ); Hee, Min-Kyung    (Research Center for Molecular Microbiology, Seoul National University   ); Kim, Chi-Kyung    (Chungbuk National University  );
  • 초록

    Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 isolated from the polluted environment are capable of degrading biphenyl and 4-chlorobiphenyl (4CB) to produce benzoic acid and 4-chlorobenzoic acid (4CBA) respectively, by pcbABCD-encoded enzymes. 4CBA can be further degraded by Pseudomonas sp. DJ-12, but not by Pseudomonas sp P20. However, the meta-cleavage activities of 2, 3-dihydroxybiphenyl (2, 3-DHBP) and 4-chloro-2, 3-DHBP dioxygenases (2, 3-DHBD) encoded by pcbC in Pseudomonas sp. P20 were stronger than Pseudomonas sp. DJ-12. In this study, the pcbC gene encoding 2, 3-DHBD was cloned from the genomic DNA of Pseudomonas sp. P20 by using pKT230. A hybrid plasmid pKK1 was constructed and E. coli KK1 transformant was selected by transforming the pKK1 hybrid plasmid carrying pcbC into E. coli XL1-Blue. By transferring the pKK1 plasmide of E. coli KK1 into Pseudomonas sp. DJ-12 by conjugation, a recombinant strain Pseudomonas sp. P20, Pseudomonas sp. DJ-12, and the recombinant cell assay methods. Pseudomonas sp. DJ12-C readily degraded 4CB and 2, 3-DHBP to produce 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA), and the resulting 4CBA and benzoic acid were continuously catabolized. Pseudomonas sp. DJ12-C degraded 1 mM 4CB completely after incubation for 20 h, but Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 showed only 90% and Pseudomonas sp. DJ-12 had, but its degradation activity to 2, 3-DHBP, 3-methylcatechol, and catechol was improved.


  • 주제어

    4-chlorobiphenyl .   degradation .   cloning of pcbC .   intragenic intragenic recombination .   Pseudomonas sp .   DJ12-E.  

  • 참고문헌 (24)

    1. Genetic structure of the genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6oxo-6-phenylhexa-2,4-dienoic acid hydrolase from biphenyl- and 4-chlorobiphenyl-degrading Pseudomonas sp. strain DJ-12 , Kim, E.;Y. Kim;C.K. KIm , Appl. Environ. Microbiol. / v.62,pp.262-267,
    2. Combination of the tod and the tol pathways in redesigning a metabolic route of Pseudomonas putida for the mineralization of a benzene, toluene, and p-xylene mixture , Lee, J.;K. Jung;S.H. Choi;H. Kim , Appl. Environ. Microbiol. / v.61,pp.2211-2217,
    3. Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation , Mondello, F.J. , J. Bacteriol. / v.171,pp.1725-1732,
    4. Sambrook, J.;E.F. Fritsch;T. Maniatis , Molecular cloning, a laboratory manual(2nd ed.) / v.,pp.,
    5. A DNA module encoding bph genes for the degradation of polychlorinated biphenyls (PCBs) , Dowling, D.N.;R. Pipke;D.F. Dwyer , FEMS Microbiol. Lett. / v.113,pp.149-154,
    6. Cloning of 2,3-dihydroxybiphenyl dioxygenase gene from Pseudomonas sp. P20 using pKT230 vector , Kim, J.Y.;C.K. Kim;J.O. Ka;K.H. Min;Y.K. Park , Kor. J. Appl. Microbiol. Biotechnol. / v.24,pp.657-663,
    7. Effectient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase , Furukawa, K.;J. Hirose;S. Hayashida;K. Nakamura , J. Bacteriol. / v.176,pp.2121-2123,
    8. Molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: some soil bacteria possess a highly conserved bph operon , Furukawa, K.;N. Hayase;K. Taira;N. Tomizuka , J. Bacteriol. / v.171,pp.5467-5472,
    9. Construction of 3-chlorbiphenyl-utilizing recombinant from an intergenic mating , Adams, R.H.;C.M. Huang;F.K. Hisgon;V. Brenner;D.D. Focht , Appl. Environ. Microbiol. / v.58,pp.647-654,
    10. Recombination of a 3-chlorbenzoate catabolic plasmid from Alcaligenes eutrophus NH9 mediated by directed repeat elements , Ogawa, N.;K. Miyashita , Appl. Environ. Microbiol. / v.61,pp.3788-3795,
    11. Cloning and expression of the polychlorinated biphenyl-degradation gene cluster from Arthrobacter M5 and comparison to analogous genes from gram-negative bacteria , Peloquin, L.;C.W. Greer , Gene / v.125,pp.35-40,
    12. Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons , Kim, C.K.;J.W. Kim;Y.C. Kim , Kor. J. Microbiol. / v.25,pp.122-128,
         
    13. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas , Bagdasarian, M.;R. Lurz;B. Ruckert;F.C.H. Franklin;M.M. Bagdasarian;J. Frey;K.N. Timmis , Gene / v.16,pp.237-247,
    14. Detection of specific segments among DNA fragments separated by gel electrophoresis , Southern, E.M. , J. Mol. Biol. / v.95,pp.503-517,
    15. intergenic natural plasmid transformation between Escherichia coli and a marine Vibraio species , Paual, J.H.;M.J. Gauther(ed) , Gene transfers and environment / v.,pp.63-67,
    16. Increased expression of the plasmid-determined 2,3-dihydroxy-biphenyl dioxygenase gene in strains of Esherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa , Andreyeva, A.L.;A.V. Slepenkyn;I.I. Starovoytov , FEMS Microbiol. Lett. / v.106,pp.211-216,
    17. Exogenous isolation of mobilizing plasmids from polluted soils and sludges , Top, E.;I.D. Smet;W. Verstraete;R. Dijkmans;m. Mergeay , Appl. Environ. Microbiol. / v.60,pp.831-839,
    18. Divergence of the cbp genes in 4-chlorobiphenyl catabolizing bacteria , Youn, D.J.;J.J. Han;C.K. Kim;Y. Kim , Kor. J. Microbiol. / v.30,pp.53-59,
         
    19. Construction of hybrid biphenyl (bph) and toluene (tod) genes for functional analysis of aromatic ring dioxygenases , Hirose, J.;A. Suyama;S. Hayashida;K. Furukawa , Gene / v.138,pp.27-33,
    20. Structure of the pcbC gene encoding 2,3-dihydroxybiphenyl dioxygenase of Pseudomonas sp. P20 , Kim, C.K.;E. Kim;J.C. Chae;Y. Kim , Biochem. Biophys. Res. Comm. / v.226,pp.15-20,
    21. Functional and structural relationship of various extradiol aromatic ringcleavage dioxygenases of Pseudomonas origin , Hirose, J.;N. Kimura;A. Suyama;A. Kobayashi;S. Hayashida;K. Furukawa , FEMS Microbiol. Lett. / v.118,pp.273-278,
    22. Conversion of chlorobiphenyls into phenylhexadienoates and benzoates by the enzymes of the upper pathway for polychlorobiphenyl degradation encoded by the bph locus of Peudomonas sp. strain LB400 , Seeger, M.;K.N. Timmis;B. Hofer , Appl. Environ. Microbiol. / v.61,pp.2654-2658,
    23. Cloning and sequencing of two tandem genes involved in degradation of 2,3-dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyl-degrading soil bacterium Pseudomonas sp. strain KKS102 , Kimbara, K.;T. Hashimoto;M. Fukuda;T. Koana;M. Takagi;M. Oishi;K. Yano , J. Bacteriol. / v.171,pp.2740-2747,
    24. Cloning and expression of pcbC and pcbC genes responsible for 2,3-dihydroxybiphenyl degradation from Pseudomonas sp. P20 , Nam, J.H.;H.M. Oh;C.K. Kim , J. Microbiol. Biotechnol. / v.5,pp.68-73,
         

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  • 이기성 (16)

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