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The journal of microbiology v.35 no.2, 1997년, pp.123 - 126  

Production of Lipocortin-1$_{1-185}$ Using A Recombinant of Escherichia coli.

Lee, Kyung-Il    (Research Center for Molecular Microbiology, Seoul National University   ); Oh, Kyung-Hee    (Research Center for Molecular Microbiology, Seoul National University   ); Lee, Jung-Hyun    (Research Center for Molecular Microbiology, Seoul National University   ); Na, Do-Sun    (Ulsan University   ); Lee, Kye-Joon    (Research Center for Molecular Microbiology, Seoul National University  );
  • 초록

    The aim of the present study was to optimize culture condition for the expression of lipocortin-1 $_{1-185}$ in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1 $_{1-185}$ gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28 $^{\circ}C$ for seed culture and 4 $0^{\circ}C$ for main culture and the optimum pH was 7.0. The production of Lipocortin-1 $_{1-185}$ was closely associated with cell growth and related to plasmid amplification.


  • 주제어

    Lipocortin-1 $_{1-185}$ E.   coli .   pHT22 .   optimization .   yield.  

  • 참고문헌 (11)

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    5. Cloning and expression of human lipocortin-1 cDNA in E. coli , Huh, K.R.;S.H. Park;S.M. Kang;I.S. Song;H.Y. Lee;D.S. Na , Kor. Biochem. Jour. / v.23,pp.459-464,
    6. The heat-shock DnaK protein is required for plasmid R1 replication and it is dispensable for plasmid ColE1 replication , Giraldo-Suarez, R.;E. Fernandez-Tresguerres;R. Diaz-Orejas;A. Malki;M. Kohiyama , Nucleic Acids Res. / v.21,pp.5495-5499,
    7. A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding , Bradford, M.M. , Anal. Biochem. / v.72,pp.248-254,
    8. Identification and characterization of mutants responsible for a runaway replication phenotype of plasmid R1 , Givskov, M.;P. Stougaard;J. Light;S. Molin , Gene / v.57,pp.203-211,
    9. Crystal structure of human annexin 1 at 2.5 Å resolution , Weng, X.;H. Luecke;I.S. Song;D.S. Kang;S.H. Kim;R. Huber , Protein Science / v.2,pp.448-458,
    10. A runaway-replication mutant of plasmid R1drd-19: temperature-dependent loss of copy number control , Uhlin, B.E.;K. Nordstrom , Mol. Gen. Genet. / v.165,pp.167-172,
    11. Effect of the bacterial growth on replication control of plasmid pBR322 in Escherichia coli , Lin-Chao, S.;H. Bremer , Mol. Gen. Genet. / v.203,pp.143-149,

 저자의 다른 논문

  • Na, Doe-Sun (20)

    1. 1991 "인간 인터루킨-2를 코드하는 유전자의 합성과 대장균에서의 발현" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 19 (3): 228~234    
    2. 1991 "Saccharomyces cerevisiae에서 인체 Lipocortin-1의 유전자 발현 및 분비" 한국생화학회지 24 (5): 453~460    
    3. 1992 "Production and Purification of Soluble Recombinant Human Lymphotoxin in Escherichia coli" Journal of microbiology and biotechnology 2 (1): 21~25    
    4. 1993 "Cell Cycle Dependent Expression of cdc2 During Rat Liver Regeneration" 한국생화학회지 26 (5): 460~464    
    5. 1993 "Asymmetric PCR: Optimization of PCR Conditions for Amplification of A cDNA Insert from ${\lambda}gt11$ Library" 한국생화학회지 26 (7): 670~672    
    6. 1995 "A Phospholipase $A_2$ Inhibitor from Arisaema amurense Max. var. serratum Nakai" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 18 (4): 293~294    
    7. 1996 "A Simple Method for Generation of Homologous Internal Standards for Competitive PCR" Journal of biochemistry and molecular biology = 한국생화학회지 29 (5): 481~483    
    8. 1997 "Lipocortin 1 Mediates the Suppressive Effects of Dexamethasone on ConA-induced Proliferative Response and Nitric Oxide Production in Rat Splenic Leukocytes" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 1 (1): 91~96    
    9. 1998 "Conformational Change of Human Annexin I by the Binding of $Ca^{2+}$, ATP and cAMP" Journal of the Korean magnetic resonance society 2 (2): 141~151    
    10. 1998 "Transient Increase of Lipocortin 1 in Nuclei of the Hippocampal Pyramidal Neurons in Rats Induced by Immobilization Stress" Journal of biochemistry and molecular biology = 한국생화학회지 31 (2): 117~122    
  • Lee, Kye-Joon (41)

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