본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The journal of microbiology v.35 no.2, 1997년, pp.134 - 140  

Purification and Characterization of an Exo-polygalacturonase from Botrytis cinerea

Lee, Tae-Ho    (Gyeongsang National University   ); Kim, Byung-Young    (Gyeongsang National University   ); Chung, Young-Ryun    (Gyeongsang National University   ); Lee, Sang-Yeol    (Gyeongsang National University   ); Lee, Chang-Won    (Gyeongsang National University   ); Kim, Jae-Won    (Gyeongsang National University  );
  • 초록

    Botrytis cinerea T91-1 has been shown to produce at least four different polygalacturonases into a liquid medium containing citrus pectin, a carbon sousrce. One of the enzymes, which had an apparent molecular weight of 66 kDa estimated by denatured polyacrylamide gel electrophoresis, was purified to electrophoretic homogeneity by a series of procedures including a cetone precipitation, ion exchange, heparin affinity, and reverse phase column chromatographies. The molecular weight of native enzyme was determined to be 64 kDa by gel permeation chromatography indicating the enzyme to be a single polypeptide chain. By viscometric analysis, the enzyme was revealed as exo-polygalacturonase. The enzyme activity was inhibited by divalent cations such as $Ca^{2+}$ , $Mg^{2+}$ , and Cu $^{2+}$ . Km and Vmax for polygalacturonic acid hydrolysis were 0.33 mg/ml and 28.6 nM/min, respectively. The optimum temperature for enzymatic activity was 5 $0^{\circ}C$ . And the enzyme showed optimal pH values between 4.0 and 5.0. The enzyme was stable upto 12 hours in the range of pH 3 to 8 and at temperature below 3 $0^{\circ}C$ .


  • 주제어

    Botrytis cinerea .   Enzyme production .   exo-polygalacturonase.  

  • 참고문헌 (27)

    1. Common amino acid domain among endopolygalacturonase of ascomycete fungi , Keon, J.P.R.;G. Wakesman , Appl. Environ. Microbiol. / v.56,pp.2522-2528,
    2. Purification and characterization of a constitutive polygalacturonase associated with the infection process of french bean leaves by Botrytis cinerea , Leone, G.;E.S.M. Schoffelmeer;J.V.D. Heuvel , Can. J. Bot. / v.68,pp.1921-1930,
    3. Purification and characterization of an endopolygalacturonase produced by Colletotrichum gloeosporioides , Prusky, D.;S. Gold;N.T. Keen , Physiol. Mol. Plant Pathol. / v.35,pp.121-133,
    4. Purification of polygalacturonase produced by Botrytis cinerea E-200 , Zalewska-Sobczak, J.;H. Urbanek , Bulletin de l'Academie polonaise des Science: Series des sciences biologiques / v.23,pp.663-658,
    5. New aspects on the infection mechanism of Botrytis cinerea Pers , Edlich, W.;G. Lorenz;H. Lyr;E. Nega;E.H. Pommer , Neth. J. Plant Pathol. / v.95,pp.53-62,
    6. Significance of polygalacturonase production by Botrytis cinerea in Pathogenesis , Leone, G.;Verhoeff, K.(ed.);N.E. Malathrakis(ed.);B. Williamson(ed.) , In Recent Advances in Botrytis Research / v.,pp.63-68,
    7. Polygalacturonase produced in Apple tissue decayed by Botrytis cinerea , Tobias, R.B.;W.S. Conway;C.E. Sams , Biochem. Mol. Biol. Int'l. / v.35,pp.813-825,
    8. An immunological study of the induction of polygalacturonases in Botrytis cinerea , Johnston, D.J.;B. Williamson , FEMS Microbiol. Lett. / v.97,pp.19-24,
    9. Host-pathogen interactions. Ⅳ. Studies on the polysaccharide-degrading enzymes secreted by Fusarium oxysporum f. sp. lycopersia , Jones, T.M.;A.J. Anderson;P. Albersheim , Physiol. Plant Pathol. / v.2,pp.153-166,
    10. A rapid and sensitive method for the determination of microgram quantities of protein utilizing the principle of protein-dye binding , Bradford, M.M. , Anal. Biochem. / v.72,pp.248-254,
    11. Host-pathogen interactions. Ⅱ. Parameters affecting polysaccharide-degrading enzyme secretion by Colletotrichum lindemathianum grown in culture , English, P.D.;J.B. Jurale;P. Albersheim , Plant Physiol. / v.47,pp.1-6,
    12. Curbitacins, 'attack' enzymes and laccase in Botrytis cinerea , Viterbo, A.;B. Yagen;A.M. Mayer , Phytochem. / v.32,pp.61-65,
    13. Presence of endo-polygalacturonase in conidia of Botrytis cinerea before and during germination , Verhoeff, K.;J.I. Liem , Phytopathol. Z. / v.91,pp.110-115,
    14. Isozymes of pectinesterase and polygalacturonase from Botrytis cinerea Pers , Colomick, S.P.;N.O. Kaplan;Wood, W.A.(ed.);S.T. Kellog(ed.) , In Methods in Enzymology / v.161,pp.329-397,
    15. Systemic infection of blaccurrant flowers by Botrytis cinerea and its possible involvement in premature abscission of fruits , McNicol, R.J.;B. Williamson , Annal. Appl. Biol. / v.114,pp.243-254,
    16. Putative virulence factors of Botrytis cinerea acting as a wound pathogen , Staples, R.C.;A.M. Mayer , FEMS Microbiol. Lett. / v.134,pp.1-7,
    17. Purification and characterization of four polygalacturonases from Botrytis cinerea , Johnston, D.J.;B. Williamson , Mycol. Res. / v.96,pp.343-349,
    18. Use of dinitrosalicylic acid reagent for determination of reducing sugar , Miller, G.L. , Anal. Chem. / v.31,pp.426-428,
    19. Partial purification and characterization of polygalacturonase produced by Botrytis cinerea , Rha, Y.J.;J.W. Kim;Y.R. Chung;N.E. Huh;K.Y. Cho , Kor. J. Plant Pathol. / v.10,pp.215-221,
    20. The role of pectic isozymes in plant pathogenesis , Collmer, A.;N.T. Keen , Annu. Rev. Phytopathol. / v.24,pp.383-409,
    21. Regulation of synthesis of cell wall-degrading enzymes by Verticillian albo-atrion and Fusarium oxysporum f. sp. lycopersia , Cooper, R.M.;R.K.S. Wood , Physiol. Plant Pathol. / v.5,pp.135-156,
    22. Cleavage of structural protein during the assembly of the head of bacteriophage T4 , Laemmli, U.K. , Nature / v.227,pp.680-685,
    23. Variability of polygalacturonase and protein isoelectric focussing patterns in Botrytis cinerea isolates , Magro, P.;L. Di;P. Marciano;C. Pallavicini , J. Gen. Microbiol. / v.120,pp.105-109,
    24. Characterization of an endopolygalacturonase produced by solid-state cultures of the aerobic fungus Penicillum capsulatum , Gillespie, A.M.;K. Cook;M.P. Coughlan , J. Biotechnol. / v.13,pp.279-292,
    25. Coley-Smith, J.R.;K. Verhoeff;W.R. Jarvis , The biology of Botrytis / v.,pp.,
    26. Pectic activities from Fusarium oxysporum f. sp. melonis: Purification and characterization of an exo-polygalacturonase , Martinez, M.J.;M.T. Alonana;F. Guillen;C. Vazquez;F. Reyes , FEMS Microbiol. Lett. / v.81,pp.145-150,
    27. Purification and characterization of two polygalacturonases secreted by Colletorichum lindemuthianum , Barthe, J.P.;D. Canteys;A. Touze , Phytopathol. Z. / v.100,pp.162-171,

 저자의 다른 논문

  • 이태호 (8)

    1. 1997 "식물 병원진균 Botrytis cinerea가 생산하는 Endo-polygalacturonase의 순수정제와 특성" 한국균학회지 = The Korean journal of mycology 25 (4): 330~339    
    2. 2013 "바이오 센서로의 응용을 위한 2차 양극산화 시간에 따른 나노튜브의 구조적, 전기적 특성" 전기전자재료학회논문지 = Journal of the Korean institute of electronic material engineers 26 (10): 741~744    
    3. 2014 "소결 온도에 따른 0.94(Na0.5K0.5)NbO3-0.06(Sr0.5Ca0.5)TiO3-0.1 MnO2의 압전 특성" 전기전자재료학회논문지 = Journal of the Korean institute of electronic material engineers 27 (1): 14~17    
  • 김병영 (1)

  • Chung, Young-Ryun (25)

  • Lee, Sang-Yeol (29)

  • 이창원 (8)

  • 김재원 (15)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기