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The journal of microbiology v.35 no.2, 1997년, pp.152 - 157  

Nitro oxide in human cytomegalovirus replication and gene expression

Lee, Jee-Yeon    (Chungbuk National University   ); Lee, Chan-Hee    (Chungbuk National University  );
  • 초록

    Infection of human fibroblast (HF) cells with human cytomegalovirus (HCMV) result in changes in the intracellular level of second messengers. Since nitric oxide (NO) production has been known to be related with other second messengers, it is probable that HCMV infection of HF cells may involve NO. To test this possibility, the amount of NO was measured following ogenous addition of NO generators such as sodium nitroprusside (SNP) or S-nitroso-N-a-cetylpenicillamine (SNAP) immediately after HCMV infection, however, inhibited virus multiplication. Furthermore, immunoblot experiment using monoclonal antibody to HCMV major immediate early (MIE) proteins or CAT assay using pCMVIE/CAT (plasmid containing CAT gene driven by HCMV MIE promoter) revealed that SNP or SNAP blocked the MIE gene expression. SNP was more effective than SNAP in hibiting HCMV multiplication or MIE gene expression. SNP produced more NO than SNAP in inhibiting HCMV multiplication or MIE gene expression. SNP produced more NO than SNAP. Although the mechanism for the inhibition of HCMV multiplication and MIE gene expression by NO is still elusive some correlation with NO-mediated inhibition of HCMV-induced increase in cytosolic free Ca $^2+$ concentration ([Ca $^2+$]) was observed. The increase of [Ca $^2+$] following HCMV infection was inhibited by SNP, and less effectively by SNAP. Raising [Ca $^2+$ with bromo-A23187 partially reversed the SNP block of MIE gene expression. Thus, there appear to e some relationships among NO. [Ca $^2+$], and HCMV MIE gene expression.


  • 주제어

    Human cytomegalovirus(HCMV) .   nitric oxide .   Ca.  

  • 참고문헌 (29)

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 저자의 다른 논문

  • Lee, Chan-Hee (31)

    1. 1993 "Human cytomegalovirus 증식에 미치는 cAMP의 영향" Korean journal of microbiology = 미생물학회지 31 (1): 72~78    
    2. 1993 "전염성 췌장 괴저 바이러스 감염에 따른 CHSE 세포의 칼슘 반응" Korean journal of microbiology = 미생물학회지 31 (1): 79~84    
    3. 1996 "신경세포에서의 Human Cytomegalovirus 증식과 이에 따른 세포내 유리칼슘 농도 변화" 대한바이러스학회지 = The journal of the Korean society of virology 26 (1): 1~8    
    4. 1997 "Transforming Growth $Factor-{\beta}$ Enhances Tyrosine Phosphorylation of Two Cellular Proteins in HEL Cells" Journal of biochemistry and molecular biology = 한국생화학회지 30 (2): 119~124    
    5. 1999 "Human Cytomegalovirus 유전자 발현에 Cyclic GMP의 영향" 대한바이러스학회지 = The journal of the Korean society of virology 29 (4): 261~269    
    6. 2000 "Herpes Simplex Virus Type-1 (HSV-1) 감염에 따른 세포내 유리 $Ca^{2+}$농도의 변화" Korean journal of microbiology = 미생물학회지 36 (4): 306~311    
    7. 2000 "Measurement of Antiviral Activities Using Recombinant Human Cytomegalovirus" The journal of microbiology 38 (4): 255~259    
    8. 2000 "Expression of Human Cytomegalovirus Immediate Early US3 Gene in Human Fibroblast Cells" The journal of microbiology 38 (1): 24~30    
    9. 2001 "인체단핵세포주 THP-1세포에서 Human Cytomegalovirus의 잠복감염과 재활성화" Korean journal of microbiology = 미생물학회지 37 (2): 145~150    
    10. 2002 "세포배양법과 PCR 방법에 의한 물에서의 폴리오 바이러스 검출" Korean journal of microbiology = 미생물학회지 38 (3): 198~204    

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