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감귤류 변패의 원인균인 Penicillium sp.-L4가 생성하는 식물세포벽 분해효소의 작용양상

김무성    ((주)태평양 기술연구원 기반기술연구소 Tel.82-331-281-8340 E-mail:prdcspch@interpia.net   ); 최영길    (한양대학교 생물학과  );
  • 초록

    Penicillium sp.-L4, a causative fungus of rot in citrus fruits, was isolated and its mode of hydrolytic enzyme production was investigated. Carboxymethylcellulase (CMCase), polygalacturonase(PGase), extra- & intra-cellular $\beta$ -glucosidase and cellobiase were produced drastically by addition of substrates in minimal media. Production of the hydrolytic enzymes were induced efficiently by cellobiose and cellooligosaccharides which were the products of cellulose hydrolysis, but repressed by addition of mono-saccharide such as glucose, raffinose, galacturonic acid. The relative activity of p-nitrophenyl- $\beta$ -D-glucopyranoside(PNPG) hydrolysis was higher than that of cellobiose hydrolysis in extracellular enzymes, and reverse is true in intracellular enzymes. Intact enzyme production of P. sp.-L4 on lemon peel lesion was sequential. $\beta$ -Glucosidase and CMCase were produced first and followed by PGase. The enzyme productivities and pH in lesions were coincident with optimal pH of each enzyme activities.


  • 주제어

    Penicillium sp.   Fungal infection .   Hydrolytic enzyme .   ${\beta}$-glucosidase.  

  • 참고문헌 (30)

    1. Fungi as parasites of plants , Landecker, E. M. , Fundamentals of the fungi(2nd ed.) / v.,pp.466-478,
    2. Characteristics of tomato cell wall degradation in vitro , Wallner, S. J.;H. L. Bloom , Plant Physiol. / v.60,pp.207-210,
    3. Purification and some properties of β-glucosidase from ectomycorrhizal fungus Pisolithus tinctorius strain , Cao, W.;D. L. Grawford , Can. J. Microbiol. / v.39,pp.125-129,
    4. β-Glucosidase from Botrytis cinerea: Its involvement in the pathgenicity of this fungus , Sasaki, I.;H. Nagayama , Biosci. Biotech. Biochem. / v.60,pp.54-56,
    5. Comparison of xylanase production by fungal pathogens of barly with reference to Bipolaris sorokiniana , Peltonen, S. , Mycol. Res. / v.99,pp.717-723,
    6. Fungal and other β-D-glucosidase; their properties and applications , Woodward, J.;A. Wiseman , Enzyme Microb. Technol. / v.4,pp.73-79,
    7. Kinetic and mathematical model of hydrolysis and transglycosylation catalyzed by cellobiase , Gusakov, A. V.;A. P. Sintisyn;G. H. Goldstein;A. A. Kylosov , Enzyme Microb. Technol. / v.6,pp.275-282,
    8. Oxalate production by fungi: its role in pathogenicity and ecology in soil environment , Dutton, M. V.;C. S. Evans , Can. J. Microbiol. / v.42,pp.881-895,
    9. Induction of electrolyte loss, tissue maceration, and cellular death of potato tissue by an endopoly-galacturonate trans-eliminase , Mount, M. S.;D. F. Bateman;H. G. Basham , Phytopathology / v.60,pp.924-931,
    10. Use of dinitrosalicylic acid reagent for determination of reducing sugars , Miller, G. L. , Anal. Biochem. / v.28,pp.353-360,
    11. Cellulase and the Rhizoctonia disease of bean , Bateman, D. F. , Phytopathology / v.54,pp.1372-1377,
    12. Chemical signals responsilble for appressorium formation in the rice blast fungus Magnaporthe grisea , Gilbert, R. D.;A. M. Johnson;R. A. Dean , Physiol. Mol. Plant. Pathol. / v.48,pp.335-346,
    13. Interaction of the β-transglycosylase of Trichoderma longibrachiatum with cellulose , Tanaka, T;S. Oi , Agric. Biol. Chem. / v.49,pp.939-944,
    14. Fungal enzymes degrading plant cell walls: their possible significance in the ectomycorrhizal symbiosis , Cairney, J. W. G;R. M. Burke , Mycol. Res. / v.98,pp.1345-1356,
    15. Purification and structural determination of a phytotoxic substance from Exerohilum turcicum , Bashan, B;R. S. Levy;M. Cojocaru;Y. Levy , Physiol. Mol. Plant Pathol. / v.47,pp.225-235,
    16. Purification and properties of two β-glucosidase from Penicillum herquei Banier and Sartory , Funaguma, T.;A. Hara , Agric. Biol. Chem. / v.52,pp.749-755,
    17. 植物病原絲狀菌による宿主植物の認識と感染 , 山口勇;植草秀敏 , 防菌防微 / v.24,pp.119-129,
    18. Hydrolysis of lignocellulose by Penicillium funiculosum cellulase , Mishra, C.;M. Rao;R. Seeta;M. C. Srinivasan;V. Deshpande , Biotechnol. Bioeng. / v.26,pp.370-373,
    19. Host-pathogen interactions , Fisher, M. L.;A. J. Anderson;P. Albersheim , Plant Physiol. / v.51,pp.489-491,
    20. 잿빛곰팡이 병원균 Botrytis cinerea 균주의 분리기 주별 표현형적 특성, 병원성 및 약제저항성 변이 , 최인실;정영륜;조광연 , 한국균학회지 / v.23,pp.246-256,
         
    21. Sequential production of polygalacturonase, cellulase, and pectin lyase by Rhizoctonia solani , Lishker, N.;J. Katan;Y. Henis , Can. J. Microbiol. / v.21,pp.1298-1304,
    22. Involvement of protein phosphorylation in the induction of approessorium formation in Collectrichum gloeosporioides by its host surface wax and ethylene , Flashiman, M. A.;C.-S. Hwang;P. E. Kolattukudy , Physiol. Mol. Plant Pathol. / v.47,pp.103-117,
    23. Notes from the underground: highlights from plant-microbe interactions , Baron, C.;P. C. Zambryski , Trends Biotechnol. / v.13,pp.356-362,
    24. The plant cell wall , Goodwin, T. W.;E. I. Mercer , Introduction to plant biochemistry(2nd ed.) / v.,pp.55-91,
    25. 眞菌の分離と分類, 同定-Penicillium屬 , 宇田川俊一 , 防菌防微 / v.19,pp.657-665,
    26. Citrus limonoid reduction of chemically induced tumorigenesis , Lam, L. K. T.;J. Zhang;S. Hasegawa , Food Technol. / v.48,pp.104-109,
    27. Invertase activity in Epichloe/Acremonium fungal endophytes and its possible role in choke disease , Lam, C. K.;F. C. Belanger;J. F. White Jr.;J. Daie , Mycol. Res. / v.99,pp.867-873,
    28. Samson, R. A.;E. S. Hoekstra;C. A. N. Van Oorschot , Introduction of food-born fungi(ed.) / v.,pp.,
    29. Plant resources of southeast asia , Verheij, E. W.;R. E. Coronel , Edible fruits and nuts(ed.) / v.,pp.119-140,
    30. β-Glucosidase production by the mycelial culture of the mushroom Termitomyces clypeatus , Sengupta, S.;S. Sengupta , Enzymer Microb. Technol. / v.12,pp.309-314,

 저자의 다른 논문

  • 최영길 (68)

    1. 1977 "미생물의 색소에 관한 연구. 제1보" Korean journal of microbiology = 미생물학회지 15 (4): 159~169    
    2. 1981 "진해만의 미생물분포 I" Korean journal of microbiology = 미생물학회지 19 (2): 45~51    
    3. 1981 "효모의 배양시기에 따른 인산화합물의 합성 및 효흡능에 미치는 탄수원의 영향" Korean journal of microbiology = 미생물학회지 19 (2): 63~77    
    4. 1984 "微生物의 色素에 關한 硏究(第 5 報) -色素形成에 미치는 界面活性劑의 영향-" Korean journal of microbiology = 미생물학회지 22 (3): 191~195    
    5. 1985 "효모 세포의 Tripolyphosphatase와 Polyphosphatase 활성도 및 Volutin 과립의 세포학적 관찰" 한국균학회지 = The Korean journal of mycology 13 (3): 141~148    
    6. 1985 "Trichoderma속의 염색체(染色體)에 관한 연구(硏究)" 한국균학회지 = The Korean journal of mycology 13 (4): 221~224    
    7. 1985 "Catabolic Repression 및 Derepression에 의한 효모 세포의 다당류 함량 변화와 무기 폴리 인산(제 5 보)" 한국균학회지 = The Korean journal of mycology 13 (4): 235~241    
    8. 1985 "Saccharomyces uvarum의 Alkaline 및 Acid Phosphatase의 Isoenzyme 양상에 대하여" Korean journal of microbiology = 미생물학회지 23 (3): 172~176    
    9. 1985 "계면활성제 첨가배양에 따른 Rhizopus oryzae의 $\alpha$-Amylase와 Phosphatase분비촉진" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 13 (4): 403~408    
    10. 1986 "Aspergillus niger의 원형질체 융합에 관한 연구" 한국균학회지 = The Korean journal of mycology 14 (2): 165~174    

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