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Aspergillus niger가 생성하는 생전분 분해효소의 정제와 특성

정만재    (충북대학교 식품공학과 Tel. 82-431-61-2566  );
  • 초록

    Aspergillus niger was selected as a strain producing the potent raw starch hydorlyzing enzyme. These experiments were conducted to investigate the conditions of the glucoa- mylase production, the purification of the enzyme, some characteristics of the purified enzyme and hydrolysis rate on various raw starches such as com, rice, potato, glutinous rice, sweet potato, wheat and barley. The optimum cultural temperature and time for the enzyme production on wheat bran medium were $30^{\circ}C$ and 96hrs, respectively. The respective addition of yeast extract and nutrient broth on wheat bran medium increased slightly the enzyme production. The enzyme was purified by ammonium sulfate fractionation and DEAE-cellulose column chromatography. The specific activity of the purified enzyme was 30.7u/mg-protein and the yield of enzyme activity was 25.8%. The purified enzyme showed a single band on polyacrylamide disc gel electrophoresis and its molecular weight was estimated to be 56,000 by SDS-polyacrylamide disc gel electrophoresis. The isoelectric point for the purified enzyme was pH3.7. The optimum temperature and pH were $65^{\circ}C$ and pH 4.0, respectively. The purified enzyme was stable in the pH range of pH 3.0-9.5 and below $45^{\circ}C$ , and its thermal stability was slightly increased by the addition of $Ca^{2+}$ . The purified enzyme was activated by $Co^{2+},\;Sr^{2+},\;Mn^{2+},\;Fe^{2+},\;Cu^{2+}$ . Raw rice starch, raw corn starch, raw glutinous rice starch, raw sweet potato starch, raw wheat starch and raw barley starch showed more than 90% hydrolysis rate in 48hrs incubation. Even raw potato starch, most difficult to be hydrolyzed, showed 80% hydrolysis rate. The purified enzyme was identified as glucoamylase.


  • 주제어

    Glucoamylase .   Hydrolysis rate .   Fractionation .   Specific activity .   Isoelectric point .   Raw starch.  

  • 참고문헌 (23)

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  • 이 논문을 인용한 문헌 (1)

    1. Park, Do-Yeun ; Park, Joong-Kook ; Cho, Sung-Back ; Kim, Chang-Hyun 2010. "Effects of Dried Whole Crop Barley Treated with Cellulolytic Microorganisms on In Vitro Fermentation Characteristics in Swine" 한국초지조사료학회지 = Journal of the Korean Society of Grassland and Forage Science, 30(2): 179~190     

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    2. 1976 "사상균(絲狀菌)의 지방분해효소(脂肪分解酵素)에 관(關)한 연구(硏究) 제2보(第2報) 분리사상균(分離絲狀菌) Rhizopus japonicus의 배양조건검토(培養條件檢討)" 한국식품과학회지 = Korean journal of food science and technology 8 (1): 33~41    
    3. 1976 "사상균(絲狀菌)의 지방분해효소(脂肪分解酵素)에 관(關)한 연구(硏究) -제3보(第3報) 분리사상균(分離絲狀菌) Rhizopus japonicus lipase의 정제(精製)에 관(關)하여" 한국식품과학회지 = Korean journal of food science and technology 8 (3): 141~146    
    4. 1976 "사상균(絲狀菌)의 지방분해효소(脂肪分解酵素)에 관(關)한 연구(硏究) -제(第)4보(報) 분리사상균(分離絲狀菌) Rhizopus japonicus의 정제(精製) Lipase의 특성(特性)에 관(關)하여-" 한국식품과학회지 = Korean journal of food science and technology 8 (4): 242~246    
    5. 1977 "Rhizopus japonicus S-62가 생성(生成)하는 단백질분해효소(蛋白質分解酵素)에 관(關)한 연구(硏究)" 한국식품과학회지 = Korean journal of food science and technology 9 (1): 31~35    
    6. 1977 "사상균의 단백질분해효소에 관한 연구 (제1보) Rhizopus japonicus S-62에 의한 산성 생산 및 내열성시험" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 5 (3): 153~158    
    7. 1978 "식품공업(食品工業)에 이용(利用)되는 효소(酵素)의 생산(生産)과 제품화(製品化)에 관(關)한 연구(硏究) -과즙(果汁)의 청징(淸澄)에 사용(使用)하는 강력(强力)한 미생물(微生物)펙틴분해효소(分解酵素)의 개발(開發)-" 한국식품과학회지 = Korean journal of food science and technology 10 (2): 237~244    
    8. 1979 "사상균의 단백질분해효소에 관한 연구 (제1보) Aspergillus awamori U-3에 의한 Acid Protease의 생산 및 효소의 특성" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 7 (3): 157~164    
    9. 1980 "Aspergillus oryzae KC-15에 의한 protease의 생산 및 그 효소의 특징에 관한 연구" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 8 (2): 77~85    
    10. 1981 "Bacillus circulans F-2가 생산하는 $\alpha$-Amylase에 관한 연구 (제 1보) $\alpha$-Amylase의 정제" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 9 (4): 185~190    

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