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Bacillus subtilis 유래 Glycerol-3-phosphate Cytidylyltransferase의 화학적 수식

박영서    (경원대학교 공과대학 식품생물공학과 Tel. 82-342-750-5378 E-mail : chanhop@chollian.dacom.co.kr  );
  • 초록

    Glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis was modified with various chemical modifiers to determine the active sites of the enzyme. Treatment of the enzyme with group-specific reagents diethylpyrocarbonate, N-bromosuccinimide, or carbodiimide resulted in complete loss of enzyme activity, which shows histidine, tryptophan, and glutamic acid or aspartic acid residues are at or near the active site. In each case, inactivation followed pseudo first-order kinetics. Inclusion of glycerol-3-phosphate and/or CTP prevented the inactivation, indicating the presence of tryptophan and glutamic acid or aspartic acid residues at the substrate binding site. Analysis of kinetics of inactivation showed that the loss of enzyme activity was due to modification of a two histidine residues, single tryptophan residue, and two glutamic acid or aspartic acid residues.


  • 주제어

    Glycerol-3-phosphate Cytidylyltransferase .   Chemical medification .   Bacillus subtilis .   Active site.  

  • 참고문헌 (15)

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