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Streptomyces lividans에서 secE 유전자의 클로닝과 염기서열 결정

김순옥    (명지대학교 자연과학연구소 생명과학과   ); 서주원    (명지대학교 자연과학연구소 생명과학과 Tel. 82-335-30-6190 E-mail : jwsuh@bioserver.myongji.ac.kr  );
  • 초록

    The secE gene of Streptomyces lividans TK24 was cloned by the polymerase chain reaction method with synthetic oligonucleo- tide primers designed on the basis of the nucleotide sequences of Streptomyces coelicolor secE-nusG-rplK operon. The deduced amino acid sequences of the SecE were highly homologous to those of other known SecE protein, that is 36.8%, 30.4%, 80.0%, and 80.9%, similarity to E. coli, Bacillus subtilis, Streptomyces griseus, Streptomyces virginiae SecE, respectively and exactly same with Streptomyces coelicolor SecE. It means that in spite of evolutionary differences, the genes for protein translocation machinery are highly conserved in eubacteria. The gene organization of secE-nusG-rplK is also similar to that of E. coli, B. subtilis, and streptomycetes.


  • 주제어

    Streptomyces lividans .   secE .   protein translocation machinery .   PCR.  

  • 참고문헌 (21)

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    2. Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature , Nishiyama, K.;M. Honda;H. Tokuda , EMBO J. / v.13,pp.3272-3277,
    3. Streptomyces coelicolor에서 secY 유전자의 클로닝과 염기서열 결정 , 김상숙;현창구;김영민;이주헌;정인권;김대명;서주원 , 한산미지 / v.23,pp.678-686,
         
    4. Identification of a new gene(secA) and gene product involved in the secretion of envelope proteins in Escherichia coli , Oliver, D. B.;J. Beckwith , J. Bacteriol. / v.150,pp.686-691,
    5. Hopwood, D. A.;M. J. Bibb;K. F. Chater;T. Kieser;C. J. Bruton;H. M. Kieser;D. J. Lydiate;C. P. Smith;J. M. Ward;H. Schrempf , Genetic manipulation of Streptomyces, A laboratory munual / v.,pp.,
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    7. Organization and nucleotide sequence of the secE-nusG region of Streptomyces griseus , Miyaka, K.;H. Onaka;S. Horinouchi;T. Beppu , Biochim. BIophys. Acta. / v.1217,pp.97-100,
    8. Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon , Downing, W. L.;S. L. Sullivan;M. E. Gottesman;P. P. Dennis , J. Bacteriol. / v.172,pp.1621-1627,
    9. secD a new gene involved in protein export in Escherichia coli , Gardel, S. C.;S. Benson;J. Hunt;S. Michaelis;J. Beckwith , J. Bacteriol. / v.169,pp.1286-1290,
    10. The secD locus of E. coli codes for two membrane proteins required for protein export , Gardel , C.;K. Johnson;A. Jacq;J. Beckwith , EMBO J. / v.13,pp.3209-3216,
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    12. 김순옥;서주원 , Streptomyces lividans에서 secY homolog의 클로닝과 분석 / v.,pp.,
    13. Cloning, nucleotide sequence, and transcriptional analysis of the nusG gene of Streptomyces coelicolor A3(2), which encodes a putative transcriptional antiterminator , Puttikhunt, C.;T. Nihira;Y. Yamada , Mol. Gen. Genet. / v.247,pp.118-122,
    14. Isolation of a sec Y homologue from Bacillus subtilis: evidence for a common protein export pathway in eubacteria , Suh, J. W.;S. A. Boylan;S. M. Thomas;K. M. Dolan;D. M. Oliver;C. W. Price , Molecular Microbiol. / v.4,pp.305-314,
    15. Isolation and characterization of the secE homologue gene of Bacillus ubtilis , Jeong, S. M.;H. Yoshikawa;H. Takahashi , Mol. Microbiol. / v.10,pp.133-142,
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    19. The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE , Flower, A. M.;R. S. Osborne;T. J. Silhavy , EMBO J. / v.14,pp.884-893,
    20. A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins , Ito, K.;M. Wittekind;M. Nomura;K. Shiba;T. Yura;A. Mjura;H. Nishimoto , Cell. / v.32,pp.789-797,
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  • 김순옥 (2)

    1. 1998 "Streptomyces lividans Tk24에서 secY homolog의 클로닝과 분석" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 26 (2): 110~116    
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