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Journal of food science and nutrition v.2 no.4, 1997년, pp.285 - 290  

Inhibitors of Tyrosinase and Melanogenesis from Galla rhois

Kim, Hyo-Jin    (Department of Food Science and Nutrition, Catholic University of Taegu-Hyosung   ); Jang, Dong-Il    (Pacific R&D Center   ); Park, Sang-Won    (Department of Food Science and Nutrition, Catholic University of Taegu-Hyosung  );
  • 초록

    Previously, a 50% aqueous methanol extract of Galla rhois was shown to be the most potent tyrosinase inhibition activity with an {TEX}$IC_{50}${/TEX}(the concentration causing 50% inhibition of tyrosinase activity) of 0.2mg/ml of 205 crude drug extracts. To isolate tyrosinase inhibitors, the methanol extract was evaporated to a small volume in vacuo, and then partitioned stepwise with benzene and ethyl acetate(EtOAc). the EtOAc fraction was solubilized in 10% MeOH solution, and then fractionated successively by Diaion HP-20 and Sephadex LH-20 column chromatography, and preparative HPLC. Three phenolic compounds were isolated, and characterized as gallic acid(GA), methyl gallate(MG) and 1,2,3,4,6-penta-O-galloyl- $\beta$ -D-glucose(PGG) by UV, IR, {TEX}${1}^H${/TEX}-&{TEX}${13}^C${/TEX}-NMR, and FAB-MS spectroscopy, PGG({TEX}$IC_{50}${/TEX}=50 $\mu\textrm{g}$ /ml) showed a considerable inhibitory effect against mushroom tyrosinase, while GA({TEX}$IC_{50}${/TEX}=1.6mg/ml) and MG({TEX}$IC_{50}${/TEX}=234 $\mu\textrm{g}$ /ml) did not show an appreciable effect. Meanwhile, MG inhibited greatly melanogenesis in a murine melanocyte cell line, Mel-Ab. MG and PGG showed typical noncompetitive inhibition patterns against mushroom tyrosinase. These results suggest that PGG and MG may be potentially useful as either anti-browning or anti-melanogenic agents in foods and cosmetics.


  • 주제어

    Galla rhois .   tyrosinase inhibitor .   melanogenesis inhibitor .   gallic acid .   methyl gallacte .   1,2,3,4,6-penta-O-galloyl- $\beta$-D-glucose.  

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    4. 2002 "Inhibition of Tyrosinase and Lipoxygenase Activities by Resveratrol and Its Derivatives from Seeds of Paeonia lactiflora" Nutraceuticals and food 7 (4): 447~450    
    5. 2002 "Cytotoxic and Antimutagenic Stilbenes from Seeds of Paeonia lactiflora" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 25 (3): 293~299    
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