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Journal of microbiology and biotechnology v.7 no.2, 1997년, pp.114 - 120   피인용횟수: 1

Characterization of an Endoxylanase Produced by an Isolated Strain of Bacillus sp.

Lee, Jay-J.    (Department of Biological Sciences, Korea Advanced Institute of Science and Technology   ); Hahm, Kyoung-Soo   (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Science and TechnologyCC0186926  ); Lee, Ki-Young   (Department of Food and Nutrition, Hoseo UniversityUU0001560  ); Lee, Sung-Taik   (Department of Biological Sciences, Korea Advanced Institute of Science and TechnologyUU0001375  );
  • 초록

    Microorganisms producing xylanase were screened for the enzymatic production of xylo-oligo saccharides from xylan. One of the bacteria isolated from compost produced an endoxylanase extracellularly. The bacterium was identified as Bacillus sp. according to its taxonomic characteristics examined. Xylanase production reached upto 5 U/ml after 22 h of culture in LB medium at $30^{\circ}C$ . The xylanase was purified by ammonium sulfate precipitation and gel filtration. The molecular weight of the xylanase was estimated to be 20,400 by SDS-PAGE. Optimal temperature and pH for the xylanase activity was $60^{\circ}C$ and 6.5, respectively. The enzyme was stable at temperatures upto $40^{\circ}C$ and pH values from 4 to 10. The xylanase was completely inhibited by the addition of 2 mM mercury ion. Apparent $K_m$ and $V_max$ values for oat spelt xylan were 9.2 mg/ml and 1954 U/mg protein, respectively. For birchwood xylan, the values were 6.3 mg/ml and 1009 U/mg protein. The predominant products of the xylan hydrolysis were xylobiose, xylotriose and xylotetraose, indicating that the enzyme is an endoxylanase. Upto $85{\%}$ of the initially added enzyme (2 U/ml) was bound to 50 mg/ml of the insoluble fraction of oat spelt xylan after incubation at $30^{\circ}C$ for 30 min.


  • 주제어

    xylanase .   Bacillus .   xylooligosaccharides.  

  • 이 논문을 인용한 문헌 (1)

    1. 2002. "" Journal of microbiology and biotechnology, 12(5): 766~772     

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  • Lee, Sung-Taik (32)

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