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The Inhibitory Mechanism of Aloe Glycoprotein (NY945) on the Mediator Release in the Guinea Pig Lung Mast Cell Activated with Antigen-Antibody Complexes

Ro, Jai-Youl   (Departments of Pharmacology, Yonsei University College of MedicineUU0000936  ); Lee, Byung-Chul   (Departments of Molecular Biology, Korea university College of Life ScienceUU0000159  ); Chung, Myung-Hee   (Department of Pharmacology, Seoul National University College of MedicineUU0000691  ); Lee, Seung-Ki   (Department of Biochemistry, Seoul National University College of PharmacyUU0000691  ); Sung, Chung-Ki   (Department of Pharmaceutics, Chunnam University College of Pharmacy  ); Kim, Kyung-Hwan   (Departments of Pharmacology, Yonsei University College of MedicineUU0000936  ); Park, Young-In   (Departments of Molecular Biology, Korea university College of Life ScienceUU0000159  );
  • 초록

    It has been reported that the glycoprotein extracted from Aloe has strong anti-inflammatory response. However, there has been no research report yet about the effect of Aloe on allergic hypersensitivity reactivity. By using guinea pig lung mast cells, this study aimed to examine the effects of Aloe glycoprotein (NY945) on the mediator releases caused by mast cell activation, and also aimed to assess the effects of NY945 on the mechanism of mediator releases in the mast cell activation. We partially purified mast cell from guinea pig lung tissues by using the enzyme digestion, the rough and the discontinuous density percoll gradient method. Mast cells were sensitized with IgG1 (anti-OA) and challenged with ovalbumin. Histamine was assayed by fluorometric analyzer, leukotrienes by radioimmunoassay. The phospholipase D activity was assessed by the production of labeled phosphatidylalcohol. The amount of mass 1, 2-diacylglycerol (DAG) was measured by the $[^3H]DAG$ produced when prelabeled with $[^3H]myristic$ acid. The phospholipid methylation was assessed by measuring the incorporation of the $[^3H]methyl$ moiety into phospholipids of cellular membranes. Pretreatment of NY945 (10 ${\mu}g$ ) significantly decreased histamine and leukotrienes releases during mast cell activation. The decrease of histamine release was stronger than that of leukotriene during mast cell activation. The phospholipase D activity increased by the mast cell activation was decreased by the dose-dependent manner in the pretreatment of NY945. The amount of DAG produced by PLC activity was decreased by NY945 pretreatment. The amount of mass 1, 2-diacylglycerol produced by activation of mast cells was decreased in the pretreatment of NY945. NY945 pretreatment strongly inhibited the incorporation of the $[^3H]methyl$ moiety into phospholipids. The data suggest that NY945 purified from Aloe inhibits in part an increase of 1, 2-diacylglycerol which is produced by activating mast cells with antigen-antibody reactions, which is mediated via phosphatidylcholine-phospholipase D and phosphatidylinositol-phospholipase C systems, and then followed by the inhibition of histamine release. Furthermore, NY945 reduces the production of phosphatidylcholine by inhibiting the methyltransferase I and II, which decreases the conversion of phosphatidylcholine into arachidonic acid and inhibits the production of leukotrienes.


  • 주제어

    Aloe glycoprotein .   Mast cells .   Histamine .   Leukotrienes .   Phospholipase D .   1, 2-diacylglycerol .   Methyltransferase.  

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  • Lee, Byung-Chul (5)

  • Chung, Myung-Hee (31)

  • 이승기 (26)

  • 김경환 (40)

  • PARK, YOUNG-IN (32)

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