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Characteristics of $Ca^{2+}$ Stores in Rabbit Cerebral Artery Myocytes

Kim, Sung-Joon   (Department of Physiology & Biophysics, Seoul National University College of MedicineUU0000691  ); Kim, Jin-Kyung   (Department of Anesthesiology, Seoul National University College of MedicineUU0000691  ); So, In-Suk   (Department of Physiology & Biophysics, Seoul National University College of MedicineUU0000691  ); Suh, Suk-Hyo   (Department of Physiology, Ewha Womans University College of MedicineUU0001056  ); Lee, Sang-Jin   (Department of Physiology, Chungbuk National University College of MedicineUU0016427  ); Kim, Ki-Whan   (Department of Physiology & Biophysics, Seoul National University College of MedicineUU0000691  );
  • 초록

    In a myocyte freshly isolated from rabbit cerebral artery, the characteristics of $Ca^{2+}$ release by histamine or caffeine were studied by microspectrofluorimetry using a $Ca^{2+}-binding$ fluorescent dye, fura-2. Histamine (5 ${\mu}M$ ) or caffeine (10 mM) induced a phasic rise of cytoplasmic free $Ca^{2+}$ concentration $([Ca^{2+}]_C)$ which could occur repetitively with extracellular $Ca^{2+}$ but only once or twice in $Ca^{2+}-free$ bathing solution. Also, the treatment with inhibitor of sarcoplasmic reticulum $Ca^{2+}-ATPase$ suppressed the rise of $[Ca^{2+}]_C$ by histamine or caffeine. In $Ca^{2+}-free$ bathing solution, short application of caffeine in advance markedly attenuated the effect of histamine, and vice versa. In normal $Ca^{2+}-containing$ solution with ryanodine (2 ${\mu}M$ ), the caffeine-induced rise of $[Ca^{2+}]_C$ occurred only once and in this condition, the response to histamine was also suppressed. On the other hand, in the presence of ryanodine, histamine could induce repetitive rise of $[Ca^{2+}]_C$ while the amplitude of peak rise became stepwisely decreased and eventually disappeared. These results suggest that two different $Ca^{2+}-release$ mechanisms (caffeine-sensitive and histamine-sensitive) are present in rabbit cerebral artery myocyte and the corresponding pools overlap each other functionally. Increase of $[Ca^{2+}]_C$ by histamine seems to partially activate ryanodine receptors present in caffeine-sensitive pool.


  • 주제어

    Smooth muscle .   $Ca^{2+}$ concentration .   $Ca^{2+}$ pool .   Histamine .   Caffeine.  

 저자의 다른 논문

  • Kim, Sung Joon (18)

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  • Kim, Jin-Kyung (1)

  • So, In-Suk (30)

  • Suh, Suk Hyo (4)

  • 김기환 (51)

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