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Cardioprotective Effects of Low Dose Bacterial Lipopolysaccharide May Not Be Directly Associated with Prostacyclin Production

Moon, Chang-Hyun   (Department of Physiology, Scool of Medicine, Ajou UniversityUU0000892  ); Kim, Ji-Young   (Department of Physiology, Scool of Medicine, Ajou UniversityUU0000892  ); Lee, Soo-Hwan   (Department of Physiology, Scool of Medicine, Ajou UniversityUU0000892  ); Baik, Eun-Joo   (Department of Physiology, Scool of Medicine, Ajou UniversityUU0000892  );
  • 초록

    Sublethal dose of bacterial lipopolysaccharide (LPS) would induce protection against cardiac ischemic/reperfusion (I/R) injury. This study examines the following areas: 1) the temporal induction of the cardio-protection produced by LPS; and 2) the relations between a degree of protection and the myocardial prostacyclin ( $PGI_2$ ) production. Rats were administered LPS (2 mg/kg, i.v.), and hearts were removed 1, 4, 8, 14, 24, 48, 72,and 96 h later. Using Langendorff apparatus, haemodynamic differences during 25 min of global ischemia/30 min reperfusion were investigated. The concentration of $PGI_2$ in aliquots of the coronary effluent was determined by radioimmunoassay as its stable hydrolysis product $6-keto-PGF1_{\alpha}$ and lactate dehydrogenase release were measured as an indicative of cellular injury. LPS-induced cardiac protection against I/R injury appeared 4 h after LPS treatment and remained until 96 h after treatment. $PGI_2$ release increased 2-3 fold at the beginning of reperfusion compared to basal level except in hearts treated with LPS for 48 and 72 h. In hearts removed 48 and 72 h after LPS treatment, basal $PGI_2$ was increased. To determine the enzymatic step in relation to LPS-induced basal $PGI_2$ production, we examined prostaglandin H synthase (PGHS) protein expression, a rate limiting enzyme of prostaglandin production, by using Western blot analysis. LPS increased PGHS protein expression in hearts at 24, 48, 72, 96 h after LPS treatment. Induction of PGHS expression appeared in both isotypes of PGHS, a constitutive PGHS-1 and an inducible PGHS-2. To identify the correlationship between $PGI_2$ production and the cardioprotective effect against I/R injury, indomethacin was administered in vivo or in vitro. Indomethacin did not inhibit LPS-induced cardioprotection, which was not affected by the duration of LPS treatment. Taken together, our results suggest that $PGI_2$ might not be the major endogenous mediator of LPS-induced cardioprotection.


  • 주제어

    Bacterial lipopolysaccharide .   Cardioprotection .   Ischemia .   Reperfusion .   Heart .   Prostacyclin.  

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  • Lee, Soo Hwan (19)

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