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Inhibitory Effect of Caffeine on Carbachol-Induced Nonselective Cationic Current in Guinea-Pig Gastric Myocytes

Kim, Sung-Joon   (Heart Research Institute, Seoul National University Medical Research CenterUU0000691  ); Min, Kyung-Wan   (Department of Physiology & Biophysics, Seoul National University College of MedicineUU0000691  ); Kim, Young-Chul   (Department of Physiology & Biophysics, Seoul National University College of MedicineUU0000691  ); Lee, Sang-Jin   (Department of Physiology, Chungbuk National University College of MedicineUU0016427  ); So, In-Suk   (Department of Physiology & Biophysics, Seoul National University College of MedicineUU0000691  ); Kim, Ki-Whan   (Department of Physiology & Biophysics, Seoul National University College of MedicineUU0000691  );
  • 초록

    In gastrointestinal smooth muscle, muscarinic stimulation by carbachol (CCh) activates nonselective cation channel current ( $I_{CCh}$ ) which is facilitated by intracellular [ $Ca^{2+}$ ] increase. Caffeine is widely used in experiments to mobilize $Ca^{2+}$ from intracellular stores. This study shows a strong inhibitory effect of caffeine on $I_{CCh}$ in guinea-pig gastric myocyte. In this study, the underlying mechanism of the inhibitory effect of caffeine was investigated. $I_{CCh}$ was completely suppressed by the addition of caffeine (10 mM) to the superfusing solution. Inhibition of $I_{CCh}$ by caffeine was not related to the intracellular cAMP accumulation which was expected from the phosphodiesterase-inhibiting effect of caffeine. The blockade of $InsP_3-induced$ $Ca^{2+}$ release by heparin had no significant effects on the activation of $I_{CCh}$ . When the same cationic current had been induced by intracellular dialysis of $GTP[{\gamma}S]$ in order to bypass the muscarinic receptor, the inhibitory effect of caffeine was significantly attenuated. The results of this study indicate that both intracellular signalling pathways for $I_{CCh}$ , proximal and distal to G-protein activation, are suppressed by caffeine. A major inhibition was observed at the proximal level.


  • 주제어

    Smooth muscle .   Caffeine .   Nonselective cationic current .   Carbachol.  

 저자의 다른 논문

  • Kim, Sung Joon (18)

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    2. 1993 "Effect of Dopamine on the $Ca^{2+}\;-dependent\;K^+\;currents$ in Isolated Single Gastric Myocytes of the Guinea-pig" 대한생리학회지 = Korean journal of physiology 27 (2): 139~150    
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    5. 1995 "Contractile and Electrical Responses of Guinea-pig Gastric Smooth Muscle to Bradykinin" 대한생리학회지 = Korean journal of physiology 29 (2): 233~241    
    6. 1997 "Effects of Arachidonic Acid on the Calcium Channel Current $(I_{Ba})$ and on the Osmotic Stretch-induced Increase of $I_{Ba}$ in Guinea-Pig Gastric Myocytes" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 1 (4): 435~443    
    7. 1998 "ATP-Sensitive $K^+$ Currents in Gastric Myocytes Isolated from Guinea-pig" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2 (1): 85~93    
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    9. 1998 "Effects of Prostaglandin $E_2$ on the Spontaneous Contractions and Electrical Activities of the Antral Circular Muscle in Guinea-pig Stomach" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2 (3): 361~368    
    10. 1998 "Sensitivity of Rabbit Cerebral Artery to Serotonin is Increased with the Moderate Increase of Extracellular $K^+$" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2 (6): 695~703    
  • So, In-Suk (30)

  • 김기환 (51)

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