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표고버섯의 미토콘드리아성 NADH 탈수소효소의 특성
Characterization of Mitochondrial NADH Dehydrogenase in Lentinus edodes

김은미   (동국대학교 이과대학 화학과UU0000452  ); 민지영   (동국대학교 생명자원과학대학 응용생물학과UU0000452  ); 민태진   (동국대학교 이과대학 화학과UU0000452  );
  • 초록

    Mitochondria were isolated from Lentinus edodes and properties of the mitochondrial NADH dehydrogenase were studied. Optimal pH, temperature, and thermal stability of the enzyme were estimated to be 7.6, $33^{\circ}C$ , and stable for one hour at $50^{\circ}C$ . The apparent $K_m$ for the NADH was 0.33 mM. This enzyme catalyzed to transfer electrons from NADH to ferricyanide, decylubiquinone, and 2,6-dichloro-phenol-indophenol. 0.5 mM antimycin A and 0.01 mM dibromothymoquinone strongly inhibited 87.8% and 76.5% of the enzyme activities. 0.01 mM oligomycin known as an inhibitor of ATPase also strongly inhibited 79.2% of activities. 0.5 mM 5,5'-dithiobis-(2-nitrobenzoic acid) and 1.0 mM N-ethylmaleimide known as a modifier of SH group inhibited 50.4% and 36.7% of activities. 1 mM ethyl 2,4-dihydroxy-6-methyl benzoate and 10 mM orcinol, which had been known as an antibiotics isolated from Umbilicaria vellea according to our previous work, stimulated 68.4% and 48.1% of the enzyme activities.


  • 주제어

    Properties .   Mitochondrial NADH dehydrogenase .   Lentinus edodes.  

  • 참고문헌 (41)

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    1. 1995 "버섯중 항균물질의 검색 및 개발에 관한 연구 -버섯중 항진균활성 물질의 검색 (I)-" 한국균학회지 = The Korean journal of mycology 23 (1): 14~27    
    2. 1996 "한국산 버섯추출물의 항진균 및 항세균활성 검색(II)" 한국균학회지 = The Korean journal of mycology 24 (1): 25~37    
    3. 1997 "버섯중 항균활성물질의 개발 -버섯중의 식물병원성 곰팡이에 대한 항균활성 물질 검색-" 한국균학회지 = The Korean journal of mycology 25 (4): 354~361    
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