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한국균학회지 = The Korean journal of mycology v.26 no.4 = no.87, 1998년, pp.496 - 501   피인용횟수: 3

송이균사(Tricholoma matsutake) 배양액의 세포외 효소 활성
The Extracellular Enzyme Activities in Culture Broth of Tricholoma matsutake

이창윤   (동국대학교 응용생물학과UU0000452  ); 홍운표   (쎌바이오텍  ); 정명준   (쎌바이오텍  ); 한영환   (동국대학교 생물학과UU0000452  );
  • 초록

    송이균사(Tricholoma matsutake DGUM 26001, DGUM 26101, DGUM 26210, FRI 91024)를 사용하여 균사의 효소활성을 측정하였다. $24^{\circ}C$ 에서 30일간 배양후 그 여액을 조효소 용액으로 사용하였을 때, ${\alpha}-amylase$ 효소의 평균 비활성은 6142.3 unit/mg protein이었다. 배양여액 중의 xylanase의 세포의 효소활성은 상대적으로 높았으나, ${\beta}-glucosidase$ , ligninase, CMCase, chitinase, pretense및 lipase의 효소활성은 낮거나 거의 없었다.


    The mycelia of Tricholoma matsutake DGUM 26001, 26101, 26210 and FRI 91024 were used to determine the extracellular enzyme activity in mycelia. When the filtrate of culture broth after 30-day cultivation at $24^{\circ}C$ was used as a crude solution of extracellular enzyme, the average specific activity of ${\alpha}-amylase$ was 6142.3 unit/mg protein. The specific activity of xylanase was comparatively high. However, little or no enzyme activities were found for ${\beta}-glucosidase$ , ligninase, CMCase, chitinase, protease, and lipase.


  • 주제어

    Tricholoma matsutake .   Exomycelial enzymes .   Broth .   Cultivationl.  

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  • 이 논문을 인용한 문헌 (3)

    1. 2000. "Characteristics of Extracellular $\beta$-Glucosidase in Tricholoma matsutake" 한국생물공학회지 = Korean journal of biotechnology and bioengineering, 15(1): 9~13     
    2. 2002. "Optimal Condition for Mycelial Growth of Beauveria bassiana and Its Extracellular Enzyme Activity" Korean journal of microbiology = 미생물학회지, 38(1): 50~53     
    3. 2004. "Physiological Characteristics and Optimized Culture Condition of the Mycelia of Inonotus mikadoi." Korean journal of microbiology = 미생물학회지, 40(2): 100~103     

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