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안정성 및 Transfection 효율이 우수한 양이온성 리포좀 유전자 전달시스템의 개발
New Cationic Liposome with Enhanced Stability and Transfection Efficiency for Gene Delivery

김경미   (원광대학교 약품연구소UU0001021  ); 남방현   (약학대학UU0001021  ); 손동환   (약학대학UU0001021  );
  • 초록

    We have developed liposomes which can be easily prepared with inexpensive lipid, have enhanced stability, and can efficiently deliver DNA into the COS-l cells, Liposome formulations were prepared using cationic materials such as dimethyldioctadecyl ammonium bromide (DDAB), cetyltrimethyl ammonium bromide(CTAB), We investigated the effect of cationic liposome formulations on in vitro DNA transfection, DDAB-containing liposomes showed increased transfection efficiency which was 3.2-fold as much as that by $Lipofectin^{\circledR}$ , but CTAB-containing liposomes were inactive in gene transfection. The effect of colipid of DDAB-containing liposome was also investegated. As a colipid, dioleylphosphatidylethanolamine(DOPE) and cholesterol did altered the transfection efficiency of DDAB-containing liposomes. And increased DDAB concentration lowered the transfection efficiency. The optimum amount of liposomal formulation was $10\;{\mu}M$ for $1\;{\mu}g$ of DNA. In the experiment of stability, DOPE-containing liposomes formulation showed a broad size distribution and separation of two major peaks on a 5th day of preparation, but liposomes containing cholesterol was stable for 10 days. DDAB-containing liposomal DNA delivery system was prepared easily and was stable.


  • 주제어

    Cationic liposome .   Gene delivery system .   Dimethyldioctadecyl ammonium bromide .   Cetyltrimethyl ammonium bromide .   COS-1 cell.  

 저자의 다른 논문

  • Kim, Kyoung-Mi (1)

    1. 1997 "Effect of ion Pairing on the Cellular Transport of Antisense Oligonucleotide" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 20 (5): 438~442    
  • Nam, Bang-Hyun (1)

  • Sohn, Dong-Hwan (38)

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