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Biotechnology and bioprocess engineering v.5 no.1, 2000년, pp.32 - 39   피인용횟수: 1

Application of a Compatible Xylose Isomerase in Simultaneous Bioconversion of Glucose and Xylose to Ethanol

Chandrakant Priya    (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology   ); Bisaria Virendra S.    (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology  );
  • 초록

    Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of glucose-xylose mixture was carried out by the yeast Saccharomyces cerevisiae in the presence of a compatible xylose isomerase. The enzyme converted xylose to xylulose and S. cerevisiae fermented xylulose, along with glucose, to ethanol at pH 5.0 and $30^{\circ}C$ . This compatible xylose isomerase from Candida boidinii, having an optimum pH and temperature range of 4.5-5.0 and $30-35^{\circ}C$ respectively, was partially purified and immobilized on an inexpensive, inert and easily available support, hen egg shell. An immobilized xylose isomerase loading of 4.5 IU/(g initial xylose) was optimum for SIF of xylose as well as SICF of glucose-xylose mixture to ethanol by S. cerevisiae. The SICF of 30 g/L glucose and 70 g xylose/L gave an ethanol concentration of 22.3 g/L with yield of 0.36 g/(g sugar consumed) and xylose conversion efficiency of $42.8\%$ .


  • 주제어

    xylose isomerase .   Saccharomyces cerevisiae .   Candida boidinii .   xylose .   ethanol fermentation .   bioconversion.  

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  • 이 논문을 인용한 문헌 (1)

    1. 2003. "" Journal of microbiology and biotechnology, 13(5): 725~730     

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