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Biotechnology and bioprocess engineering v.5 no.1, 2000년, pp.57 - 60   피인용횟수: 1

Screening of Microorganisms Producing Esterase for the Production of $(R)-\beta-Acetylmercaptoisobutyric$ Acid from Methyl $(R,S)-\beta-Acetylmercaptoisobutyrate$

Gokul Boyapati    (Biotechnology Research Centre, Indian Institute of Technology   ); Lee Je-Hyuk    (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology   ); Song Ki-Bang    (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology   ); Panda T.    (Biotechnology Research Centre, Indian Institute of Technology   ); Rhee Sang-Ki    (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology   ); Kim Chul-Ho    (Microbial & Bioprocess Engineering Laboratory, Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology  );
  • 초록

    $(R)-\beta-acetylmercaptoisobutyric$ acid (RAM), a chiral compound, is an important intermediate for the chemical synthesis of various antihypertensive and congestive heart failure drugs. Microorganisms capable of converting $(R,S)-\beta-acetylmercaptoisobutyric$ acid ((R,S)-ester) to RAM were screened from soil microorganisms. A strain of Pseudomonas sp. 1001 screened from a soil sample was selected to be the best. Cells showed an activity of 540 U/mL from culture broth and the enzyme was thermostable up to $70^{\circ}C$ . This strain could produce RAM asymmetrically from (R,S)-ester.


  • 주제어

    Pseudomonas sp.   .   esterase .   enzymatic resolution.  

  • 참고문헌 (11)

    1. Ohashi, N., S. Nagata, and S. Katsube , Japan Kokai Tokyo Koho / v.81,pp.7756,
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    3. Design of specific inhibitors of angiotensin-converting enzyme: New class of orally active antihypertensive agents. , Ondetti, M. A., B. Rubin, and D. W. Cushman , Science / v.196,pp.441-444,
    4. Process conditions for production of D-β-acetylthioisobutyric acid from methyl DL-β-acetylthioisobutyrate with the cells of Pseudomonas putida MR-2068. , Akihiro, S., O. Eiji, T. Hiroko, O. Naoto, N. Ryozo, M. Itsumi, H. Eiichi, and O. Hisao , Biosci. Biotech. Biochem. / v.57,pp.782-786,
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    7. Bifunctional chiral synthons via microbiological methods. 1. Optically active 2,4-dimethylglutaric acid monomethyl esters. , Chen, C.-S., Y. Fujimoto, and C. J. Shi , J. Am. Chem. Soc. / v.103,pp.3580-3582,
    8. Iwao, J., M. Oya, E. Kato, and T. Watanabe , Japan Kokai Tokyo Koho / v.78,pp.151912,
    9. Screening of microorganisms producing D-β-acetylthioisobutyric acid from methyl DL-β-acetylthioisobutyrate. , Akihiro, S., H. Akihiko, K. Etsuko, O. Naoto, N. Ryozo, W. Ichiro, and O. Hisoa , Biosci. Biotech. Biochem. / v.56,pp.1252-1256,
    10. Samayama, T., H. Kinugasa, H. Nishimura, K. Takeyama, and K. Hosoki , Japan Patent / v.63,pp.18599,
    11. Chemical racemization of methyl L-β-acetylthioisobutyrate. , Akihiro, S., K. Yoshimasa, N. Ohsuga, R. Numazawa, and H. Ohnishi , Biosci. Biotech. Biochem. / v.57,pp.17-19,
  • 이 논문을 인용한 문헌 (1)

    1. 2001. "" Biotechnology and bioprocess engineering, 6(3): 179~182     

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  • 송기방 (1)

    1. 1999 "Transgenic Tobacco Plants Expressing the Bacterial Levansucrase Gene Show Enhanced Tolerance to Osmotic Stress" Journal of microbiology and biotechnology 9 (2): 213~218    
  • Kim, Chul-Ho (14)

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